Human Lipase I Antibody Summary
Accession # Q6XZB0
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human Lipase I by Western Blot. Western blot shows lysates of human testis tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Lipase I Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5909) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Lipase I at approximately 54 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Lipase I
Lipase I (Lipase member I; also mPA‑PLA1 beta LPDL and PLA1C) is a 55 kDa member of the PLA1 subfamily, pancreatic lipase family, AB hydrolase superfamily of enzymes. It is expressed by sperm, where it is apparently secreted and subsequently binds to membrane-associated HSPGs. Lipase I hydrolyzes phosphatidic acid to generate 2‑acyl‑LPA, which then activates one of four G-protein coupled receptors. Human Lipase I precursor is 460 amino acids (aa) in length. It contains a 15 aa signal sequence, followed by an active catalytic site composed of Ser159, Asp183 and His268. Lipase I is known to be phosphorylated on Ser25. There are two isoform variants, both of which show a 34 aa substitution for aa 1‑13, and only one of which shows an eight aa substitution for aa 245‑460. Over aa 14‑154, human Lipase I shares 61% aa identity with mouse Lipase I.
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