Human Lipase I Antibody

Catalog # Availability Size / Price Qty
Detection of Human Lipase I by Western Blot.
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Product Details
Supplemental Products

Human Lipase I Antibody Summary

Species Reactivity
Detects human Lipase I in Western blots.
Polyclonal Goat IgG
Antigen Affinity-purified
E. coli-derived recombinant human Lipase I
Accession # Q6XZB0
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human Lipase I antibody by Western Blot. View Larger

Detection of Human Lipase I by Western Blot. Western blot shows lysates of human testis tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Lipase I Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5909) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Lipase I at approximately 54 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Lipase I

Lipase I (Lipase member I; also mPA‑PLA1 beta  LPDL and PLA1C) is a 55 kDa member of the PLA1 subfamily, pancreatic lipase family, AB hydrolase superfamily of enzymes. It is expressed by sperm, where it is apparently secreted and subsequently binds to membrane-associated HSPGs. Lipase I hydrolyzes phosphatidic acid to generate 2‑acyl‑LPA, which then activates one of four G-protein coupled receptors. Human Lipase I precursor is 460 amino acids (aa) in length. It contains a 15 aa signal sequence, followed by an active catalytic site composed of Ser159, Asp183 and His268. Lipase I is known to be phosphorylated on Ser25. There are two isoform variants, both of which show a 34 aa substitution for aa 1‑13, and only one of which shows an eight aa substitution for aa 245‑460. Over aa 14‑154, human Lipase I shares 61% aa identity with mouse Lipase I.

Entrez Gene IDs
149998 (Human); 288322 (Rat)
Alternate Names
Cancer/testis antigen 17; CT17; CT17membrane-associated phospholipase A1 beta; EC 3.1.1; EC 3.1.1.-; EC; EC; Lipase I; lipase, member I; LIPI; LPD lipase; LPDL; LPDLlipase member I; Membrane-associated phosphatidic acid-selective phospholipase A1-beta; mPA-PLA1 beta; PRED5

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Goat IgG (H+L) Antibody


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