Lectin-like oxidized low-density-lipoprotein receptor-1 (LOX-1), also known as oxidized low-density-lipoprotein receptor-1 (OLR-1), is a type II transmembrane receptor belonging to the C-type lectin family (1). It also belongs to the functionally defined scavenger receptor (SR) superfamily, whose members share the common ability to bind and internalize modified forms of Low Density Lipoproteins (LDL) (2-4). LOX-1 is the first member of the class E scavenger receptor subfamily (SR-E). It binds and supports the internalization of multiple structurally unrelated macromolecules including oxidized LDL, advanced glycation end products (AGE), activated platelets, bacteria, apoptotic or aged cells, and heat shock proteins (5-7). LOX-1 has also been implicated as an intestinal receptor involved in the transcytosis of pancreatic bile salt-dependent lipase (8). The human LOX-1 gene encodes a 273 amino acid (aa) residue protein with a short N-terminal intracellular domain, a transmembrane domain, an extracellular stalk/neck region followed by a C-type lectin-like domain (CTLD) (1, 6). The CTLD, which is required for ligand recognition, contains the six conserved cysteine residues present in all C-type lectins, but lacks the Ca2+-binding residues found in classical C-type lectins. LOX-1 can be detected on activated endothelial cells, vascular smooth muscle cells, macrophages, intestinal cells and dendritic cells (6-8). The expression of LOX-1 is induced by proinflammatory or proatherogenic stimuli, as well as by oxidized LDL itself and hemodynamic or oxidative stress. Human LOX-1 exists on the cell surface as covalent homodimers, which can further associate into non-covalent-linked oligomers (9). Cell surface LOX-1 can also be cleaved by yet unidentified proteases to release the soluble LOX-1 extracellular domain (6). Binding and endocytosis of oxidized LDL by LOX-1 induces oxidative stress, activates NF kappa B, and upregulates the expression of monocyte chemoattractant protein-1 and matrix metalloproteases (5 - 9). LOX-1-dependent oxidized LDL uptake also induces apoptosis by inducing the expression of the pro-apoptotic Bax and downregulation of the anti-apoptotic Bcl-2 (10). Oxidized LDL plays a key role in the pathogenesis of atherosclerosis and endothelial dysfunction. Blockade of LOX-1 functions may turn out to be a suitable target for the therapeutic intervention of atherosclerosis.
Key Product Details
Species Reactivity
Human
Applications
ELISA Capture (Matched Antibody Pair), Blockade of Receptor-ligand Interaction
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 331219
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Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human LOX-1
Ser61-Gln273
Accession # P78380
Ser61-Gln273
Accession # P78380
Specificity
Detects human LOX-1 in ELISAs. In ELISAs, this antibody does not cross-react with recombinant mouse LOX-1.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Applications for Human LOX‑1/OLR1 Antibody
Application
Recommended Usage
Blockade of Receptor-ligand Interaction
In a functional ELISA, 0.5 - 2.5 μg/mL of this antibody will block 50% of the binding of 1 μg/mL of biotinylated AGE-BSA to immobilized recombinant human LOX-1 coated at 5 μg/mL (100 µL/well). At 20 μg/mL, this antibody will block >90% of the binding.
Human LOX-1/OLR1 Sandwich Immunoassay
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 2 reviews rated 5 using MAB17981 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: LOX-1/OLR1
References
- Sawamura, T. et al. (1997) Nature 386:73.
- Daugherty, A. et al. (2000) Curr. Opin. Cardiovasc. Pulm. Ren. Invest. Drugs. 2:223.
- Platt, N. and S. Gordon (2001) J. Clin. Invest. 108:649.
- Platt, N. and S. Gordon (1998) Chem. Biol. 5:R193.
- Jono, T. et al. (2002) FEBS Lett. 511:170.
- Kume, N. et al. (2001) Curr. Opin. Lipidol. 12:419.
- Delneste, Y. et al. (2002) Immunity 17:353.
- Bruneau, N. et al. (2003) Mol. Biol. Cell 14:2861.
- Xie, Q. et al. (2004) DNA and Cell Biol. 23:111.
- Chen, J. et al. (2003) Circ. Res. 94:370.
Long Name
Lectin-like Oxidized Low-density Lipoprotein Receptor 1
Alternate Names
CLEC8A, LOX1, OLR1, SCARE1, SR-E1
Gene Symbol
OLR1
UniProt
Additional LOX-1/OLR1 Products
Product Documents for Human LOX‑1/OLR1 Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human LOX‑1/OLR1 Antibody
For research use only
Related Research Areas
Customer Reviews for Human LOX‑1/OLR1 Antibody (2)
5 out of 5
2 Customer Ratings
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Application: ELISASample Tested: Recombinant proteinSpecies: HumanVerified Customer | Posted 07/09/2018Human recombinant LOX-1 was coated on the plate and MAB17981 was used to detect the protein
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Application: ImmunohistochemistrySample Tested: Heart tissueSpecies: HumanVerified Customer | Posted 08/18/2017
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