Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human

Cited:

Human, Porcine

Applications

Validated:

Immunohistochemistry, Western Blot, Blockade of Receptor-ligand Interaction

Cited:

Western Blot, Neutralization, Immunocytochemistry, ELISA Detection

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all LOX-1/OLR1 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human LOX-1 (R&D Systems, Catalog # 1798-LX)
Ser61-Gln273
Accession # P78380

Specificity

Selected for its ability to block receptor-ligand interaction in a functional ELISA assay. In direct ELISAs, less than 5% cross‑reactivity with recombinant mouse LOX-1 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Human LOX‑1/OLR1 Antibody

LOX-1/OLR1 antibody in Human Placenta by Immunohistochemistry (IHC-P).

LOX‑1/OLR1 in Human Placenta.

LOX-1/OLR1 was detected in immersion fixed paraffin-embedded sections of human placenta using Goat Anti-Human LOX-1/OLR1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1798) at 1 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytotrophoblasts. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Detection of Human LOX-1/OLR1 by Immunocytochemistry/Immunofluorescence

Detection of Human LOX-1/OLR1 by Immunocytochemistry/Immunofluorescence

Generation of stable prostate cancer cell lines with LOX-1 over-expression and shRNA against olr1.A) Western blot for LOX-1 (40 kDa) expression in human CaP clones with overexpression of LOX-1. B) Western blot for LOX-1 (40 kDa) expression in human prostate cancer cell clones with LOX-1 knockdown C) Real-time PCR for LOX-1 expression in three clones with overexpression of LOX-1. D) Real-time PCR for LOX-1 expression was determined in three clones that express shRNA/LOX-1(A), and three clones that express shRNA/LOX-1(B). The data represent the means ± S.D. of three independent experiments performed in triplicate, and statistically analyzed using one-way analysis of variance and Dunnett’s post-test; (***p≤0.001, **p≤0.01, *p≤0.05). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/25170920), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Human LOX-1/OLR1 by Western Blot

Detection of Human LOX-1/OLR1 by Western Blot

Generation of stable prostate cancer cell lines with LOX-1 over-expression and shRNA against olr1.A) Western blot for LOX-1 (40 kDa) expression in human CaP clones with overexpression of LOX-1. B) Western blot for LOX-1 (40 kDa) expression in human prostate cancer cell clones with LOX-1 knockdown C) Real-time PCR for LOX-1 expression in three clones with overexpression of LOX-1. D) Real-time PCR for LOX-1 expression was determined in three clones that express shRNA/LOX-1(A), and three clones that express shRNA/LOX-1(B). The data represent the means ± S.D. of three independent experiments performed in triplicate, and statistically analyzed using one-way analysis of variance and Dunnett’s post-test; (***p≤0.001, **p≤0.01, *p≤0.05). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/25170920), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Human LOX-1/OLR1 by Western Blot

Detection of Human LOX-1/OLR1 by Western Blot

Generation of stable prostate cancer cell lines with LOX-1 over-expression and shRNA against olr1.A) Western blot for LOX-1 (40 kDa) expression in human CaP clones with overexpression of LOX-1. B) Western blot for LOX-1 (40 kDa) expression in human prostate cancer cell clones with LOX-1 knockdown C) Real-time PCR for LOX-1 expression in three clones with overexpression of LOX-1. D) Real-time PCR for LOX-1 expression was determined in three clones that express shRNA/LOX-1(A), and three clones that express shRNA/LOX-1(B). The data represent the means ± S.D. of three independent experiments performed in triplicate, and statistically analyzed using one-way analysis of variance and Dunnett’s post-test; (***p≤0.001, **p≤0.01, *p≤0.05). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/25170920), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Human LOX-1/OLR1 by Knockdown Validated

Detection of Human LOX-1/OLR1 by Knockdown Validated

Generation of stable prostate cancer cell lines with LOX-1 over-expression and shRNA against olr1.A) Western blot for LOX-1 (40 kDa) expression in human CaP clones with overexpression of LOX-1. B) Western blot for LOX-1 (40 kDa) expression in human prostate cancer cell clones with LOX-1 knockdown C) Real-time PCR for LOX-1 expression in three clones with overexpression of LOX-1. D) Real-time PCR for LOX-1 expression was determined in three clones that express shRNA/LOX-1(A), and three clones that express shRNA/LOX-1(B). The data represent the means ± S.D. of three independent experiments performed in triplicate, and statistically analyzed using one-way analysis of variance and Dunnett’s post-test; (***p≤0.001, **p≤0.01, *p≤0.05). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/25170920), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human LOX‑1/OLR1 Antibody

Application
Recommended Usage

Blockade of Receptor-ligand Interaction

In a functional ELISA, 0.5 - 2 μg/mL of this antibody will block 50% of the binding of 1 μg/mL of biotinylated AGE-BSA to immobilized recombinant human LOX-1 coated at 5 μg/mL (100 µL/well). At 10 μg/mL, this antibody will block >95% of the binding.

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human placenta

Western Blot

0.1 µg/mL
Sample: Recombinant Human LOX‑1/OLR1 (Catalog # 1798-LX)

Reviewed Applications

Read 3 reviews rated 4 using AF1798 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: LOX-1/OLR1

Lectin-like oxidized low-density-lipoprotein receptor-1 (LOX-1), also known as oxidized low-density-lipoprotein receptor-1 (OLR-1), is a type II transmembrane receptor belonging to the C-type lectin family (1). It also belongs to the functionally defined scavenger receptor (SR) superfamily, whose members share the common ability to bind and internalize modified forms of Low Density Lipoproteins (LDL) (2 - 4). LOX-1 is the first member of the class E scavenger receptor subfamily (SR-E). It binds and supports the internalization of multiple structurally unrelated macromolecules including oxidized LDL, advanced glycation end products (AGE), activated platelets, bacteria, apoptotic or aged cells, and heat shock proteins (5 - 7). LOX-1 has also been implicated as an intestinal receptor involved in the transcytosis of pancreatic bile salt-dependent lipase (8). The human LOX-1 gene encodes a 273 amino acid (aa) residue protein with a short N-terminal intracellular domain, a transmembrane domain, an extracellular stalk/neck region followed by a C-type lectin-like domain (CTLD) (1, 6). The CTLD, which is required for ligand recognition, contains the six conserved cysteine residues present in all C-type lectins, but lacks the Ca2+-binding residues found in classical C-type lectins. LOX-1 can be detected on activated endothelial cells, vascular smooth muscle cells, macrophages, intestinal cells and dendritic cells (6 - 8). The expression of LOX-1 is induced by proinflammatory or proatherogenic stimuli, as well as by oxidized LDL itself and hemodynamic or oxidative stress. Human LOX-1 exists on the cell surface as covalent homodimers, which can further associate into non-covalent-linked oligomers (9). Cell surface LOX-1 can also be cleaved by yet unidentified proteases to release the soluble LOX-1 extracellular domain (6). Binding and endocytosis of oxidized LDL by LOX-1 induces oxidative stress, activates NF kappa B, and upregulates the expression of monocyte chemoattractant protein-1 and matrix metalloproteases (5 - 9). LOX-1-dependent oxidized LDL uptake also induces apoptosis by inducing the expression of the pro-apoptotic Bax and downregulation of the anti-apoptotic Bcl-2 (10). Oxidized LDL plays a key role in the pathogenesis of atherosclerosis and endothelial dysfunction. Blockade of LOX-1 functions may turn out to be a suitable target for the therapeutic intervention of atherosclerosis.

References

  1. Sawamura, T. et al. (1997) Nature 386:73. 
  2. Daugherty, A. et al. (2000) Curr. Opin. Cardiovasc. Pulm. Ren. Invest. Drugs. 2:223. 
  3. Platt, N. and S. Gordon (2001) J. Clin. Invest. 108:649. 
  4. Platt, N. and S. Gordon (1998) Chem. Biol. 5:R193. 
  5. Jono, T. et al. (2002) FEBS Lett. 511:170. 
  6. Kume, N. et al. (2001) Curr. Opin. Lipidol. 12:419.
  7. Delneste, Y. et al. (2002) Immunity 17:353.
  8. Bruneau, N. et al. (2003) Mol. Biol. Cell 14:2861.
  9. Xie, Q. et al. (2004) DNA and Cell Biol. 23:111.
  10. Chen, J. et al. (2003) Circ. Res. 94:370.

Long Name

Lectin-like Oxidized Low-density Lipoprotein Receptor 1

Alternate Names

CLEC8A, LOX1, OLR1, SCARE1, SR-E1

Entrez Gene IDs

4973 (Human); 108078 (Mouse)

Gene Symbol

OLR1

UniProt

P78380

Additional LOX-1/OLR1 Products

Product Documents for Human LOX‑1/OLR1 Antibody

Certificate of Analysis

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Product Specific Notices for Human LOX‑1/OLR1 Antibody

For research use only

Citations for Human LOX‑1/OLR1 Antibody

Customer Reviews for Human LOX‑1/OLR1 Antibody (3)

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  • Human LOX-1/OLR1 Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Recombinant protein and Human recombinant antibody
    Species: Human
    Verified Customer | Posted 07/09/2018
    Human LOX‑1/OLR1 Antibody AF1798
  • Human LOX-1/OLR1 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: CHO Chinese hamster ovary cell line and Recombinant protein
    Species: Human
    Verified Customer | Posted 07/09/2018
  • Name: Anonymous
    Application: Western Blot
    Sample Tested: See PMID 23950953
    Species: Other
    Verified Customer | Posted 01/05/2015

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