Human Ly6K Antibody Summary
Accession # Q17RY6
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Ly6K in HeLa Human Cell Line by Flow Cytometry. HeLa human cervical epithelial carcinoma cell line was stained with Mouse Anti-Human Ly6K Monoclonal Antibody (Catalog # MAB6648, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Ly6K (Lymphocyte antigen 6 locus K; also CO16) is a 26-27 kDa member of the Ly-6 antigen family of molecules. It has restricted expression, being described in testis and skin. Ly6K is found in/on carcinomas, and is known to circulate in normal and tumor-patient blood. Mature human Ly6K is a 121 amino acid (aa) GPI-linked (presumed) glycoprotein. It is synthesized as a 165 aa preproprecursor that contains a 17 aa signal sequence, a 121 aa mature region (aa 18-138), and a 27 aa C‑terminal propeptide. There are two potential splice variants, one that shows a 48 aa substitution, and another that shows a 33 aa substitution for aa 73-165. It is not clear if mouse Ly6K is a true molecular ortholog of human Ly6K. In any event, over aa 18-138, human Ly6K shares 39% aa identity with mouse Ly6K.
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