Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 1124642
View all MAGEA3 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human MAGE-A3   pro2-Glu314  Accesssion # P43357

Specificity

Detects recombinant human MAGE-A3 protein in Direct ELISA.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human MAGEA3 Antibody (1124642)

Detection of Human MAGEA3 by Western Blot.

Western Blot shows lysates of HT1080 human fibrosarcoma cell line. PVDF membrane was probed with 2 µg/ml of Mouse Anti-Human MAGEA3 Monoclonal Antibody (Catalog # MAB11839) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for MAGEA3 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Immersion fixed paraffin-embedded sections of human lung tumor

MAGEA3 was detected in immersion fixed paraffin-embedded sections of human lung tumor using Mouse Anti-Human MAGEA3 Monoclonal Antibody (Catalog # MAB11839) at 5 µg/ml overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (VCTS021). Tissue was stained using the HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF007) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm and nucleus. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Immersion fixed paraffin-embedded sections of human melanoma

MAGEA3 was detected in immersion fixed paraffin-embedded sections of human melanoma using Mouse Anti-Human MAGEA3 Monoclonal Antibody (Catalog # MAB11839) at 5 µg/ml overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (VCTS021). Tissue was stained using the HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF007) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm and nucleus. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human MAGEA3 Antibody (1124642)

Application
Recommended Usage

Immunohistochemistry

0.25-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human lung tumor and melanoma

Western Blot

2 µg/mL
Sample: HT1080 human fibrosarcoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute lyophilized material at 0.2 mg/ml in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: MAGEA3

Melanoma-associated antigen-A3 (MAGE-A3) is a member of the MAGE (Melanoma Antigen Gene) protein family, with a molecular weight of approximately 43 kDa. The MAGE protein family is characterized by conserved domains and plays critical roles in cellular processes such as transcription regulation, apoptosis, and adaptation to cellular stress. MAGE-A3 is typically expressed in various malignancies, including melanoma and lung cancer, while exhibiting limited expression in normal tissues, primarily restricted to the testis. Due to its tumor-specific expression and immunogenic properties, MAGE-A3 has garnered significant interest as a target for cancer immunotherapy. Aberrant regulation of MAGE-A3 is associated with cancer progression, contributing to immune evasion and promoting tumor cell growth and survival. Recent studies have highlighted the role of MAGE-A3 in interacting with the ubiquitin-proteasome system, implicating it in protein degradation pathways that influence oncogenic signaling. These attributes underscore the importance of MAGE-A3 as a biomarker for both diagnostic and therapeutic applications in oncology.

References

  1. Chomez, P., De Backer, O., Bertrand, M., De Plaen, E., Boon, T., & Lucas, S. (2001). An overview of the MAGE gene family with the identification of all human members of the family. Cancer Research, 61(14), 5544-5551.
  2. Lucas, S., Brasseur, F., Demoulin, B., & Boon, T. (1997). Identification of a new MAGE gene family. Experimental Cell Research, 232(2), 418-424. doi: 10.1006/excr.1997.3561Jiricny J. The multifaceted mismatch-repair system. Nat Rev Mol Cell Biol. 2006 May;7(5):335-46. doi: 10.1038/nrm1907. PMID: 16612326.
  3. Van den Eynde, B. J., van der Bruggen, P. (1997). T cell defined tumor antigens. Current Opinion in Immunology, 9(5), 684-693.

Long Name

Melanoma-Associated Antigen 3

Alternate Names

CT1.3, MAGE3

Entrez Gene IDs

4102 (Human)

Gene Symbol

MAGEA3

Additional MAGEA3 Products

Product Documents for Human MAGEA3 Antibody (1124642)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human MAGEA3 Antibody (1124642)

For research use only

Related Research Areas

Cancer Stem Cell Markers

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Protocols

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