Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 828806
View all Matrilin-1 Primary Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human Matrilin-1
Ser23-Val496
Accession # P21941

Specificity

Detects human Matrilin-1 in ELISAs. In direct ELISAs, no cross-reactivity with recombinant human Matrilin-2, -3, or -4 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human Matrilin-1 Antibody

Matrilin-1 antibody in Human Mesenchymal Stem Cells by Immunocytochemistry (ICC).

Matrilin-1 in Human Mesenchymal Stem Cells.

Matrilin-1 was detected in immersion fixed human mesenchymal stem cells differentiated into chondrocytes using Mouse Anti-Human Matrilin-1 Monoclonal Antibody (Catalog # MAB7878) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cartilage matrix. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human Matrilin-1 Antibody

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed human mesenchymal stem cells differentiated into chondrocytes

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Matrilin-1

Matrilin-1, also known as Cartilage Matrix Protein (CMP) is a 50-60 kDa extracellular matrix protein that belongs to the superfamily of von Willebrand factor A (VWA) containing proteins. It is primarily expressed in cartilage and functions as a bridging component between proteins of the collagenous matrix. Mature human Matrilin-1 contains two VWA domains that flank one EGF-like repeat, followed by a C-terminal coiled coil domain. The Matrilins differ in the number of VWA domains (one or two) and EGF-like repeats (one, three, four, or ten) they contain. Human Matrilin-1 shares 91% aa sequence identity with human and rat Matrilin-1. The coiled coil domain of Matrilin-1 mediates disulfide-linked homo-trimer formation. It can also assemble into hetero-oligomers with Matrilin-2, -3, and -4.

Alternate Names

CMP, CRTM, MATN1, Matrilin1

Entrez Gene IDs

4146 (Human); 17180 (Mouse); 297894 (Rat)

Gene Symbol

MATN1

UniProt

P21941 (Human)

Additional Matrilin-1 Products

Product Documents for Human Matrilin-1 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Matrilin-1 Antibody

For research use only

Citations for Human Matrilin-1 Antibody

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