Human MCPH1 Antibody

Catalog # Availability Size / Price Qty
AF3998
AF3998-SP
Detection of Human MCPH1 by Western Blot.
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Product Details
Citations (4)
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Human MCPH1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human, mouse and rat MCPH1 in Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human MCPH1
Met1-Lys250
Accession # Q8NEM0
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Human MCPH1 by Western Blot. View Larger

Detection of Human MCPH1 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, CHP-100 human neuroblastoma cell line, PC-12 rat adrenal pheochromocytoma cell line, Rat-2 rat embryonic fibroblast cell line, and Neuro-2A mouse neuroblastoma cell line. PVDF membrane was probed with 1 µg/mL of Human MCPH1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3998) followed by HRP‑conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for MCPH1 at approximately 105 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MCPH1

MCPH1 (microcephalin 1), originally identified as an inhibitor of hTERT expression, has been implicated in DNA damage response. MCPH1 contains one N-terminal and two C-terminal BRCT domains. BRCT domains are found predominantly in cell cycle proteins responsive to DNA damage. MCPH1 forms irradiation-induced nuclear foci (IRIF) that colocalize with NBS1, 53BP1, MDC1, and ATM and are abolished with MCPH1 specific siRNA. MCPH1 also regulates the ATR pathway. It colocalizes with ATR and RPA and is required for the phosphorylation of RPA and Rad17. Defects in MCPH1 are the cause of primary microcephaly 1, an autosomal recessive neurodevelopmental disorder.

Long Name
Microcephalin 1
Entrez Gene IDs
79648 (Human); 244329 (Mouse)
Alternate Names
BRCT-repeat inhibitor of TERT expression 1; BRIT1; FLJ12847; MCPH1; MCT; microcephalin 1; Microcephalin; microcephaly, primary autosomal recessive 1

Product Datasheets

Citations for Human MCPH1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Targeted Next-Generation Sequencing Identifies a Recurrent Mutation in MCPH1 Associating with Hereditary Breast Cancer Susceptibility
    Authors: T Mantere, R Winqvist, S Kauppila, M Grip, A Jukkola-Vu, A Tervasmäki, K Rapakko, K Pylkäs
    PLoS Genet., 2016;12(1):e1005816.
    Species: Human
    Sample Types: Cell Lysates
    Applications: WB
  2. Targeted Next-Generation Sequencing Identifies a Recurrent Mutation in MCPH1 Associating with Hereditary Breast Cancer Susceptibility.
    Authors: Mantere T, Winqvist R, Kauppila S, Grip M, Jukkola-Vuorinen A, Tervasmaki A, Rapakko K, Pylkas K
    PLoS Genet, 2016;12(1):e1005816.
    Species: Human
    Sample Types: Cell Lysates
    Applications: WB
  3. A novel MCPH1 isoform complements the defective chromosome condensation of human MCPH1-deficient cells.
    Authors: Gavvovidis I, Rost I, Trimborn M, Kaiser F, Purps J, Wiek C, Hanenberg H, Neitzel H, Schindler D
    PLoS ONE, 2012;7(8):e40387.
    Species: Human
    Sample Types: Cell Lysates
    Applications: WB
  4. Microcephalin and pericentrin regulate mitotic entry via centrosome-associated Chk1.
    Authors: Tibelius A, Marhold J, Zentgraf H, Heilig CE, Neitzel H, Ducommun B, Rauch A, Ho AD, Bartek J, Kramer A
    J. Cell Biol., 2011;185(7):1149-57.
    Species: Human
    Sample Types: Cell Lysates
    Applications: WB

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Secondary Antibodies

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