Human MCPH1 Antibody
Human MCPH1 Antibody Summary
Met1-Lys250
Accession # Q8NEM0
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Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human MCPH1 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, CHP-100 human neuroblastoma cell line, PC-12 rat adrenal pheochromocytoma cell line, Rat-2 rat embryonic fibroblast cell line, and Neuro-2A mouse neuroblastoma cell line. PVDF membrane was probed with 1 µg/mL of Human MCPH1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3998) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for MCPH1 at approximately 105 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: MCPH1
MCPH1 (microcephalin 1), originally identified as an inhibitor of hTERT expression, has been implicated in DNA damage response. MCPH1 contains one N-terminal and two C-terminal BRCT domains. BRCT domains are found predominantly in cell cycle proteins responsive to DNA damage. MCPH1 forms irradiation-induced nuclear foci (IRIF) that colocalize with NBS1, 53BP1, MDC1, and ATM and are abolished with MCPH1 specific siRNA. MCPH1 also regulates the ATR pathway. It colocalizes with ATR and RPA and is required for the phosphorylation of RPA and Rad17. Defects in MCPH1 are the cause of primary microcephaly 1, an autosomal recessive neurodevelopmental disorder.
Product Datasheets
Citations for Human MCPH1 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 4
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Targeted Next-Generation Sequencing Identifies a Recurrent Mutation in MCPH1 Associating with Hereditary Breast Cancer Susceptibility
Authors: T Mantere, R Winqvist, S Kauppila, M Grip, A Jukkola-Vu, A Tervasmäki, K Rapakko, K Pylkäs
PLoS Genet., 2016;12(1):e1005816.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Targeted Next-Generation Sequencing Identifies a Recurrent Mutation in MCPH1 Associating with Hereditary Breast Cancer Susceptibility.
Authors: Mantere T, Winqvist R, Kauppila S, Grip M, Jukkola-Vuorinen A, Tervasmaki A, Rapakko K, Pylkas K
PLoS Genet, 2016;12(1):e1005816.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
A novel MCPH1 isoform complements the defective chromosome condensation of human MCPH1-deficient cells.
Authors: Gavvovidis I, Rost I, Trimborn M, Kaiser F, Purps J, Wiek C, Hanenberg H, Neitzel H, Schindler D
PLoS ONE, 2012;7(8):e40387.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Microcephalin and pericentrin regulate mitotic entry via centrosome-associated Chk1.
Authors: Tibelius A, Marhold J, Zentgraf H, Heilig CE, Neitzel H, Ducommun B, Rauch A, Ho AD, Bartek J, Kramer A
J. Cell Biol., 2011;185(7):1149-57.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
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