MAPK/ERK kinase kinase 3 (MEKK3), also known as MEK kinase 3 and MAP3K3, is a widely expressed 71 kDa Ser/Thr kinase in the SEK, MEK, and MyD88‑IRAK‑TRAF6 signal transduction pathways. It contains a PB1 domain (aa 45‑123), a region with several phosphorylation sites, and a protein kinase domain (aa 361‑621). MEKK3 is involved in tissue morphogenesis, inflammation, and the response to cellular stress. Its activation leads to the activation of NF kappa B following stimulation of IL-1 R, TLR4, TLR8, TNF RI, and the AT1 angiotensin receptor.
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Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Human
Applications
Knockout Validated, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 576240
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Product Specifications
Immunogen
E. coli-derived recombinant human MEKK3
Ser43-Tyr275
Accession # Q99759
Ser43-Tyr275
Accession # Q99759
Specificity
Detects human MEKK3 in direct ELISAs and Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human MEKK3 Antibody (576240)
Detection of Human MEKK3 by Western Blot.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, Daudi human Burkitt's lymphoma cell line, and A431 human epithelial carcinoma cell line. PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human MEKK3 Monoclonal Antibody (Catalog # MAB6095) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for MEKK3 at approximately 71 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Western Blot Shows Human MEKK3 Specificity by Using Knockout Cell Line.
Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and MEKK3 knockout HeLa cell line (KO). PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human MEKK3 Monoclonal Antibody (Catalog # MAB6095) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for MEKK3 at approximately 75 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of MEKK3 by Western Blot
MEKK3 is essential for embryonic vascular development and neonatal brain vascular integrity.(i) Immunoblots show expression of VEGFR2, MEKK3 and T18/S19 phosphorylated MLC2 (MLC2pT18/S19) in freshly isolated BMEC from three Mekk3NCL and three Mekk3 iEC−/− littermate pups as indicated. GAPDH expression is shown as loading controls. Elevated MLC2pT18/S19 was observed in three independently isolated pairs. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/26235885), licensed under a CC-BY license. Not internally tested by R&D Systems.Applications for Human MEKK3 Antibody (576240)
Application
Recommended Usage
Knockout Validated
MEKK3
is specifically detected in HeLa human cervical epithelial carcinoma parental cell line but is not detectable in
MEKK3 knockout HeLa cell line.
Western Blot
1 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line, Daudi human Burkitt's lymphoma cell line, and A431 human epithelial carcinoma cell line
Sample: HeLa human cervical epithelial carcinoma cell line, Daudi human Burkitt's lymphoma cell line, and A431 human epithelial carcinoma cell line
Reviewed Applications
Read 4 reviews rated 4 using MAB6095 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: MEKK3
Long Name
Mitogen-activated Protein Kinase Kinase Kinase 3
Alternate Names
MAP3K3, MAPKKK3
Gene Symbol
MAP3K3
UniProt
Additional MEKK3 Products
Product Documents for Human MEKK3 Antibody (576240)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human MEKK3 Antibody (576240)
For research use only
Related Research Areas
Citations for Human MEKK3 Antibody (576240)
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Customer Reviews for Human MEKK3 Antibody (576240) (4)
4 out of 5
4 Customer Ratings
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Application: MicroarraysSample Tested: EDTA PlasmaSpecies: HumanVerified Customer | Posted 01/14/2021
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Application: MicroarraysSample Tested: EDTA PlasmaSpecies: HumanVerified Customer | Posted 11/14/2018
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Application: ELISASample Tested: Serum and PlasmaSpecies: HumanVerified Customer | Posted 11/11/2018
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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