Human Mena Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects endogenous human Mena in Western blots.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human Mena
    Asn386-Arg513
    Accession # Q8N8S7
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.2 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human Mena by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line, MDA-MB-453 human breast cancer cell line, HUVEC human umbilical vein endothelial cells, and T47D human breast cancer cell line. PVDF membrane was probed with 0.2 µg/mL of Human Mena Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF5304) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). Specific bands were detected for Mena at approximately 80 and 88 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Mena

Mena (mammalian ENA; also protein enabled homolog) is a cytosolic member of the Ena/VASP family of proteins. It runs anomalously high in SDS-PAGE due to a high proline content. Multiple cell types produce Mena, including fibroblasts, myoepithelial cells, neurons and endothelium. Mena promotes the organization of actin filaments in filopodia, and strengthens cell-cell interactions in epithelia. Human Mena is 591 amino acids (aa) in length. It contains one WH1 domain (aa 1‑111), two coiled-coil regions (aa 135‑265 and 557‑587), a Pro-rich segment (aa 310‑373) and an EVH domain (aa 391‑588) that binds G- and F-actin, and mediates tetramerization. There is a 140 kDa neuronal isoform that shows a large 228 aa insertion after Ala268.

  • Entrez Gene IDs:
    55740 (Human); 360891 (Rat)
  • Alternate Names:
    ENA; enabled homolog (Drosophila); ENAH; FLJ10773; Mena; MENANDPP1; NDPP1; protein enabled homolog
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Normal Goat IgG Control

Ctrl AB-108-C 191  
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Secondary Antibodies
Description Application Cat# Citations Images  

Goat IgG HRP-conjugated Antibody

WB, Simple Western HAF109 19  
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Goat IgG HRP-conjugated Antibody

WB HAF017 15  
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Goat IgG (H+L) PE-conjugated Antibody

Flow F0107 4  
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Donkey Anti-Goat IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL001 8
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Donkey Anti-Goat IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL003 5
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Goat IgG (H+L) APC-conjugated Antibody

Flow F0108 6  
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Goat IgG Horseradish Peroxidase-conjugated Antibody

WB HAF019 6  
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Donkey Anti-Goat IgG Antibody

WB AF109 6
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Donkey Anti-Goat IgG Biotinylated Antibody

WB BAF109 3
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Goat IgG VisUCyte HRP Polymer

IHC VC004  
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Goat IgG (H+L) Fluorescein-conjugated Antibody

Flow F0109 2  
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Donkey Anti-Goat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL002
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Rabbit Anti-Goat IgG (H+L) Affinity Purified PAb, X Absorbed

WB, ELISA R-401-C-ABS
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Rabbit Anti-Goat IgG Biotinylated Antibody

WB BAF017
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Chicken Anti-Goat IgG Biotinylated Antibody

WB BAF019
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Donkey Anti-Goat IgG (H+L) PerCP-conjugated Antibody

Flow F0124
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