Detection of Human Mena by Western Blot.
Western blot shows lysates of MCF-7 human breast cancer cell line, MDA-MB-453 human breast cancer cell line, HUVEC human umbilical vein endothelial cells, and T47D human breast cancer cell line. PVDF membrane was probed with 0.2 µg/mL of Human Mena Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF5304) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). Specific bands were detected for Mena at approximately 80 and 88 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Mena (mammalian ENA; also protein enabled homolog) is a cytosolic member of the Ena/VASP family of proteins. It runs anomalously high in SDS-PAGE due to a high proline content. Multiple cell types produce Mena, including fibroblasts, myoepithelial cells, neurons and endothelium. Mena promotes the organization of actin filaments in filopodia, and strengthens cell-cell interactions in epithelia. Human Mena is 591 amino acids (aa) in length. It contains one WH1 domain (aa 1‑111), two coiled-coil regions (aa 135‑265 and 557‑587), a Pro-rich segment (aa 310‑373) and an EVH domain (aa 391‑588) that binds G- and F-actin, and mediates tetramerization. There is a 140 kDa neuronal isoform that shows a large 228 aa insertion after Ala268.
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