Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Mena Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Mena
Asn386-Arg513
Accession # Q8N8S7

Specificity

Detects endogenous human Mena in Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human Mena Antibody

Detection of Human Mena antibody by Western Blot.

Detection of Human Mena by Western Blot.

Western blot shows lysates of MCF-7 human breast cancer cell line, MDA-MB-453 human breast cancer cell line, HUVEC human umbilical vein endothelial cells, and T47D human breast cancer cell line. PVDF membrane was probed with 0.2 µg/mL of Human Mena Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5304) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). Specific bands were detected for Mena at approximately 80 and 88 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.

Applications for Human Mena Antibody

Application
Recommended Usage

Western Blot

0.2 µg/mL
Sample: MCF-7 human breast cancer cell line, MDA-MB-453 human breast cancer cell line, HUVEC human umbilical vein endothelial cells, and T47D human breast cancer cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Mena

Mena (mammalian ENA; also protein enabled homolog) is a cytosolic member of the Ena/VASP family of proteins. It runs anomalously high in SDS-PAGE due to a high proline content. Multiple cell types produce Mena, including fibroblasts, myoepithelial cells, neurons and endothelium. Mena promotes the organization of actin filaments in filopodia, and strengthens cell-cell interactions in epithelia. Human Mena is 591 amino acids (aa) in length. It contains one WH1 domain (aa 1‑111), two coiled-coil regions (aa 135‑265 and 557‑587), a Pro-rich segment (aa 310‑373) and an EVH domain (aa 391‑588) that binds G- and F-actin, and mediates tetramerization. There is a 140 kDa neuronal isoform that shows a large 228 aa insertion after Ala268.

Alternate Names

ENAH, NDPP1

Entrez Gene IDs

55740 (Human); 360891 (Rat)

Gene Symbol

ENAH

UniProt

Q8N8S7

Additional Mena Products

Product Documents for Human Mena Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human Mena Antibody

For research use only

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