Menin (Multiple endocrine neoplasia type I) is a 75‑78 kDa tumor‑suppressor protein that has no apparent structural relationship to any known protein family. It is ubiquitously expressed and interacts with other molecules, including NF kappa B, FANCD2 and ASK. Menin appears to block gene transcription, promote DNA repair, and inhibit cell proliferation. Human Menin is 615 amino acids (aa) in length. Consistent with its unique molecular taxonomy, it contains no definitive structural module(s). However, it is known to be phosphorylated on Ser399 and Thr599. There are a number of potential splice variants. Individually or in combination, there are deletions of aa 89‑95, 149‑153, 171‑173, 189‑223, and 423‑426. There is also a 50 aa substitution for aa 68‑615, a 66 aa substitution for aa 383‑615, and a two aa substitution for aa 507‑536. Over aa 555‑615, human and mouse Menin are completely identical in aa sequence.
Key Product Details
Species Reactivity
Applications
Label
Antibody Source
Product Specifications
Immunogen
aa 555-615
Accession # O00255
Specificity
Clonality
Host
Isotype
Scientific Data Images for Human Menin Antibody
Detection of Human Menin by Western Blot.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and MOLT-4 human acute lymphoblastic leukemia cell line. Gels were loaded with 30 µg of whole cell lysate (WCL), 20 µg of cytoplasmic (Cyto), and 10 µg of nuclear extracts (Nuc). PVDF Membrane was probed with 2 µg/mL of Goat Anti-Human Menin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6005) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Menin at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Applications for Human Menin Antibody
Western Blot
Sample: HeLa human cervical epithelial carcinoma cell line and MOLT‑4 human acute lymphoblastic leukemia cell line
Formulation, Preparation, and Storage
Purification
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Shipping
Stability & Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: Menin
Long Name
Alternate Names
Gene Symbol
UniProt
Additional Menin Products
Product Documents for Human Menin Antibody
Certificate of Analysis
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Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human Menin Antibody
For research use only
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars