Human MEPE/OF45 Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human MEPE/OF45 in direct ELISAs and Western blots.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human MEPE/OF45
    Pro18-Asp525
    Accession # Q9NQ76
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Human MEPE (Catalog # 3140-ME)
  • Neutralization
    Measured by its ability to neutralize MEPE-mediated adhesion of the ATDC5 mouse chondrogenic cell line. The Neutralization Dose (ND50) is typically 2-6 µg/mL in the presence of 2 µg/mL Recombinant Human MEPE.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Cell Adhesion Mediated by MEPE and Neutralization by Human MEPE Antibody. Recombinant Human MEPE (Catalog # 3140-ME), immobil­ized onto a microplate, supports the adhesion of the ATDC5 mouse chondrogenic cell line in a dose-dependent manner (orange line). Adhesion elicited by Recombinant Human MEPE (2 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human MEPE Antigen Affinity-purified Poly­clonal Antibody (Catalog # AF3140). The ND50 is typically
2‑6 µg/mL.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: MEPE/OF45

MEPE (matrix extracellular phosphoglycoprotein), known as OF45 in mouse and rat, is a 55 kDa member of the SIBLING protein family. MEPE is primarily expressed in bone and dentin, where it regulates the mineralization of those tissues (1‑3). The human MEPE cDNA encodes a 525 amino acid (aa) precursor that includes a 17 aa signal sequence. MEPE contains multiple consensus sites for post-translational modifications, including N-linked glycosylation, N-myristoylation, glycosaminoglycan attachment, and phosphorylation by a variety of kinases. MEPE also contains several putative proteolytic cleavage sites and one integrin-binding RGD motif (3, 4). There is therefore considerable potential for post-translational regulation of MEPE function and its degradation products. MEPE is secreted by osteoblasts and dental pulp stem cells during the mineralization process (5‑7) and also by nonmineralizing tissues including epithelial cells in the renal proximal tubule and salivary duct (8, 9). MEPE has an inhibitory function in bone formation, (5) although a peptide corresponding to aa 242‑264 stimulates new bone formation and the proliferation of osteoblasts and dental pulp stem cells (10, 11). MEPE contains one C‑terminal ASARM motif common to SIBLING proteins. Similar to intact MEPE, the ASARM peptide inhibits bone mineralization and plays a central role in the phosphaturia and reduced mineralization of X-linked hypophosphatemic rickets (HYP) and tumor‑induced osteomalacia (TIO) (12, 13). The zinc metalloprotease Phex binds directly to MEPE via the ASARM motif and prevents ASARM cleavage (13, 14). Multiple inactivating mutations in Phex are found in HYP and TIO and result in the increased liberation of ASARM peptide (15). Both MEPE and ASARM peptide are elevated in these disorders of mineralization and phosphate metabolism (12).

  • References:
    1. Fisher, L.W. and N.S. Fedarko (2003) Connect. Tiss. Res. 44:33.
    2. Quarles, L.D. (2003) Am. J. Physiol. 285:E1.
    3. Qin, C. et al. (2004) Crit. Rev. Oral Biol. Med. 15:126.
    4. Rowe, P.S.N. et al. (2000) Genomics 67:54.
    5. Gowen, L.C. et al. (2003) J. Biol. Chem. 278:1998.
    6. Siggelkow, H. et al. (2004) Bone 35:570.
    7. Liu, H. et al. (2005) Arch. Oral Biol. 50:923.
    8. Ogbureke, K.U.E. and Fisher, L.W. (2005) Kidney Int. 68:155.
    9. Ogbureke, K.U.E. and L.W. Fisher (2004) J. Dent. Res. 83:664.
    10. Hayashibara, T. et al. (2004) J. Bone Miner. Res. 19:455.
    11. Liu, H. et al. (2004) J. Dent. Res. 83:496.
    12. Bresler, D. et al. (2004) J. Endocrinol. 183:R1.
    13. Rowe, P.S.N. et al. (2005) Bone 36:33.
    14. Guo, R. et al. (2002) Biochem. Biophys. Res. Commun. 297:38.
    15. Rowe, P.S. (2004) Crit. Rev. Oral Biol. Med.15:264.
  • Long Name:
    Matrix Extracellular Phosphoglycoprotein with ASARM Motif
  • Entrez Gene IDs:
    56955 (Human); 94111 (Mouse); 79110 (Rat)
  • Alternate Names:
    extracellular phosphoglycoprotein with ASARM motif (bone); matrix extracellular phosphoglycoprotein; MEPE; OF45; Osteoregulin
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