Human MESP1 Antibody

Catalog # Availability Size / Price Qty
MAB92193-100
MAB92193-SP
Detection  of Human MESP1 by Western Blot.
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Human MESP1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human MESP-1 in direct ELISAs.
Source
Monoclonal Mouse IgG1 Clone # 939826
Purification
Protein A or G purified from cell culture supernatant
Immunogen
E. coli-derived recombinant human MESP-1
Met1-Gln85
Accession # Q9BRJ9
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Intracellular Staining by Flow Cytometry
0.25 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection  of Human MESP1 by Western Blot. View Larger

Detection of Human MESP1 by Western Blot. Western blot shows lysates of Mouse ES cells transfected with human MESP1 untreated (-) or treated (+) with 100 ng/mL Doxycycline overnight. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human MESP1 Monoclonal Antibody (Catalog # MAB92193) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for MESP1 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

Cells provided courtesy of Michael Kyba's lab, University of Minnesota.

Western Blot Detection  of Human MESP1 by Western Blot. View Larger

Detection of Human MESP1 by Western Blot. Western blot shows lysates of H1 human embryonic stem cells untreated (-) or treated (+) with 100 ng/mL Doxycycline overnight. PVDF membrane was probed with Mouse Anti-Human MESP1 Monoclonal Antibody (Catalog # MAB92193) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for MESP1 at approximately 30 kDa (as indicated).

Image provided courtesy of Michael Kyba's lab, University of Minnesota.

Intracellular Staining by Flow Cytometry Detection  of MESP1 in Mouse ES cells transfected with human MESP1 by Flow Cytometry. View Larger

Detection of MESP1 in Mouse ES cells transfected with human MESP1 by Flow Cytometry. Mouse ES cells transfected with human MESP1 were stained with Mouse Anti-Human MESP1 Monoclonal Antibody (Catalog # MAB92193, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MESP1

Mesoderm posterior protein 1 (Mesp-1) is a 268 amino acids protein that in humans is encoded by the MESP1 gene. Mesp-1 was first identified in transcripts enriched in the posterior region of the mouse embryo at embryonic day E7 to E7.5. Lineage tracing in mice showed that Mesp-1 represents the earliest marker of cardiac progenitors and directs multipotential cardiovascular cell fates, patterning mesoderm into cardiac, hematopoietic, or skeletal myogenic progenitors in a context-dependent manner.

Long Name
Mesoderm Posterior BHLH Transcription Factor 1
Entrez Gene IDs
55897 (Human); 17292 (Mouse); 308766 (Rat)
Alternate Names
bHLHc5; bHLHc5MGC10676; Class C basic helix-loop-helix protein 5; mesoderm posterior 1 homolog (mouse); mesoderm posterior protein 1; MESP1

Product Datasheets

FAQs

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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