|Detection of Human MGMT by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and MCF‑7 human breast cancer cell line. PVDF membrane was probed with 0.5 µg/mL of Human MGMT Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF3794) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for MGMT at approximately 23 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.|
The O-6-methylguanine-DNA-methyl transferase (MGMT) is involved in the direct repair of DNA alkylated at the O(6) position of guanine. MGMT transfers the alkyl group to its own cysteine thereby inactivating the MGMT molecule. Therefore, the number of MGMT molecules correlates directly with the cell’s ability to withstand DNA alkylation.
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Showing Results 1 - 1 of 1
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.
The document you requested is not available online. Please enter the Catalog Number and Lot Number below to have a document emailed to you at the address provided