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Human Miz-1/ZBTB17 Antibody

Catalog #: AF3760
2 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF3760
AF3760-SP

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Detection of Human Miz‑1/ZBTB17 by Western Blot.
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Citations (2)
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Human Miz-1/ZBTB17 Antibody Summary

Species Reactivity
Human
Specificity
Detects endogenous human Miz-1 in Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human Miz-1
Met1-Ala188
Accession # Q13105
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human Miz‑1/ZBTB17 antibody by Western Blot. View Larger

Detection of Human Miz‑1/ZBTB17 by Western Blot. Western blot shows lysates of Raji human Burkitt's lymphoma cell line and CEM human T-lymphoblastoid cell line. PVDF membrane was probed with 1 µg/mL of Human Miz-1/ZBTB17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3760) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Miz-1/ZBTB17 at approximately 100 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Miz-1/ZBTB17

Miz-1 (Myc-interacting zinc finger protein 1; also ZBTB17) is an 88 kDa, DNA-binding protein that belongs to the Kruppel C2H2-type zinc finger protein family. Human Miz-1 is 803 amino acids (aa) in length, and contains a POZ domain (aa 1 - 104), two Myc-interaction segments (aa 269 - 308 & 637 - 718), and 12 C2H2-type zinc finger motifs (aa 306 - 637); also, there is one alternate splice form that shows an eight aa substitution for Thr769. Miz-1 induces cell arrest at G1, an effect mediated by its activation of the gene coding for p15INK4b. This effect is blocked by Myc, which displaces transcriptional coactivators bound to Miz-1. Notably, although the downregulation of Miz-1 may contribute to Myc-induced cell transformation, the deactivation of Miz-1 is absolutely essential for Myc-induced apoptosis. Over aa 1‑188, human Miz-1 is 85% aa identical to mouse Miz-1.

Long Name
Myc-interacting Zinc Finger Protein-1
Entrez Gene IDs
7709 (Human); 22642 (Mouse); 313666 (Rat)
Alternate Names
MIZ1; Miz-1; Myc-interacting zinc finger protein 1; mZ13; pHZ-67; ZBTB17; Zfp100; zinc finger and BTB domain containing 17; zinc finger and BTB domain-containing protein 17; zinc finger protein 151 (pHZ-67); Zinc finger protein 151; Zinc finger protein 60MIZ-1; ZNF151; ZNF151Myc-interacting Zn finger protein-1; ZNF60

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Citations for Human Miz-1/ZBTB17 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Target genes of the largest human SWI/SNF complex subunit control cell growth.
    Authors: Inoue H, Giannakopoulos S, Parkhurst CN
    Biochem. J., 2011-02-15;434(1):83-92.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Identification of the transcription factor Miz1 as an essential regulator of diphthamide biosynthesis using a CRISPR-mediated genome-wide screen
    Authors: J Liu, Z Zuo, M Zou, T Finkel, S Liu
    PLoS Genet, 2020-10-15;16(10):e1009068.

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