Human MMP‑25/MT6-MMP Hemopexin Domain Antibody

Name: Human MMP‑25/MT6-MMP Hemopexin Domain Antibody
Brand: R&D Systems
SKU: MAB11421-SP
Rating: 5 (2 reviews)

Catalog #: MAB11421 2 Reviews Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB11421
MAB11421-SP

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human MMP‑25/MT6-MMP Hemopexin Domain Antibody Summary

Species Reactivity

Human

Specificity

Detects human MMP‑25/MT6-MMP Hemopexin Domain in Western blots. It recognizes E. coli-expressed hemopexin domain and
NS0‑expressed recombinant human MMP-25.

Source

Monoclonal Mouse IgG₁ Clone # 141825

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human MMP‑25
Pro314-Cys508
Accession # Q9NPA2

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Human MMP‑25/MT6-MMP Hemopexin Domain

Immunohistochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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MMP‑25/MT6‑MMP in Human Lung. MMP-25/MT6-MMP was detected in immersion fixed paraffin-embedded sections of human lung using Mouse Anti-Human MMP-25/MT6-MMP Hemopexin Domain Monoclonal Antibody (Catalog # MAB11421) at 8 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counter-stained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MMP-25/MT6-MMP

Matrix Metalloproteinase 25 (MMP-25), also known as MT6-MMP or Leukolysin, is a GPI-linked protease expressed by neutrophils. The extracellular domain of MMP-25 consists of a pro region, a catalytic domain and a hemopexin domain. MMP-25 cleaves collagen type 4, gelatin, fibronectin and fibrin.

Long Name

Matrix Metalloproteinase 25/Membrane Type 6 MMP

Entrez Gene IDs

64386 (Human)

Alternate Names

Leukolysin; matrix metallopeptidase 25; matrix metallopeptidase-like 1; matrix metalloproteinase 20; matrix metalloproteinase 25; matrix metalloproteinase-25; matrix metalloproteinase-like 1; membrane-type 6 matrix metalloproteinase; membrane-type matrix metalloproteinase 6; membrane-type-6 matrix metalloproteinase; MMP20; MMP20A; MMP25; MMP-25; MMPL1; MT6MMP; MT6-MMP; MT-MMP 6; MTMMP6; MT-MMP6