Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 141825
View all MMP-25/MT6-MMP Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human MMP‑25
Pro314-Cys508
Accession # Q9NPA2

Specificity

Detects human MMP‑25/MT6-MMP Hemopexin Domain in Western blots. It recognizes E. coli-expressed hemopexin domain andNS0‑expressed recombinant human MMP-25.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human MMP‑25/MT6-MMP Hemopexin Domain Antibody

MMP-25/MT6-MMP antibody in Human Lung by Immunohistochemistry (IHC-P).

MMP‑25/MT6‑MMP in Human Lung.

MMP-25/MT6-MMP was detected in immersion fixed paraffin-embedded sections of human lung using Mouse Anti-Human MMP-25/MT6-MMP Hemopexin Domain Monoclonal Antibody (Catalog # MAB11421) at 8 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counter-stained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human MMP‑25/MT6-MMP Hemopexin Domain Antibody

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human lung

Western Blot

1 µg/mL
Sample: Human MMP‑25/MT6-MMP Hemopexin Domain

Reviewed Applications

Read 2 reviews rated 5 using MAB11421 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: MMP-25/MT6-MMP

Matrix Metalloproteinase 25 (MMP-25), also known as MT6-MMP or Leukolysin, is a GPI-linked protease expressed by neutrophils. The extracellular domain of MMP-25 consists of a pro region, a catalytic domain and a hemopexin domain. MMP-25 cleaves collagen type 4, gelatin, fibronectin and fibrin.

Long Name

Matrix Metalloproteinase 25/Membrane Type 6 MMP

Alternate Names

Leukolysin, MMP25, MT6-MMP

Entrez Gene IDs

64386 (Human)

Gene Symbol

MMP25

UniProt

Q9NPA2

Additional MMP-25/MT6-MMP Products

Product Documents for Human MMP‑25/MT6-MMP Hemopexin Domain Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human MMP‑25/MT6-MMP Hemopexin Domain Antibody

For research use only

Related Research Areas

Metalloproteases and Regulators

Customer Reviews for Human MMP‑25/MT6-MMP Hemopexin Domain Antibody (2)

5 out of 5
2 Customer Ratings
5 Stars
100%
4 Stars
0%
3 Stars
0%
2 Stars
0%
1 Stars
0%

Have you used Human MMP‑25/MT6-MMP Hemopexin Domain Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Customer Images

Showing  1 - 2 of 2 reviews Showing All
Filter By:
Clear Filters X
  • Human MMP‑25/MT6-MMP Hemopexin Domain Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Lung tissue
    Species: Human
    Verified Customer | Posted 04/06/2022
    Human MMP‑25/MT6-MMP Hemopexin Domain Antibody MAB11421
  • Human MMP‑25/MT6-MMP Hemopexin Domain Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Kidney tissue
    Species: Human
    Verified Customer | Posted 01/18/2022
    Human MMP‑25/MT6-MMP Hemopexin Domain Antibody MAB11421

There are no reviews that match your criteria.

Showing  1 - 2 of 2 reviews Showing All

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies