Human MMP-9 Antibody

Catalog # Availability Size / Price Qty
AF911
AF911-SP
MMP‑9 in NS0 Mouse Cell Line.
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Product Details
Citations (11)
FAQs
Supplemental Products
Reviews (5)

Human MMP-9 Antibody Summary

Species Reactivity
Human
Specificity
Detects human and mouse MMP-9 in Western blots, dot blots, and human MMP-9 by immunocytochemistry. In Western blots, less than 1% cross-reactivity with recombinant human MMP-1, -2, -3, -7, -8, -10, -12, and -13 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human MMP-9
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human MMP‑9 Western Blot Standard (Catalog # WBC018)
Immunohistochemistry
5-15 µg/mL
See below
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Human MMP‑9 (Catalog # 911-MP), see our available Western blot detection antibodies
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Immunocytochemistry MMP‑9 in NS0 Mouse Cell Line. View Larger

MMP‑9 in NS0 Mouse Cell Line. MMP‑9 was detected in immersion fixed NS0 mouse myeloma cell line transfected with MMP-9 using 5 µg/mL Human MMP‑9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF911) for 3 hours at room temperature. Cells were stained (red) and counterstained (green). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunohistochemistry MMP‑9 in Human Ovarian Cancer Tissue. View Larger

MMP‑9 in Human Ovarian Cancer Tissue. MMP‑9 was detected in immersion fixed paraffin-embedded sections of human ovarian cancer tissue using Human MMP‑9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF911) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Immunocytochemistry MMP‑9 in MCF‑7 Human Cell Line. View Larger

MMP‑9 in MCF‑7 Human Cell Line. MMP‑9 was detected in immersion fixed MCF‑7 human breast cancer cell line using Human MMP‑9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF911) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunohistochemistry MMP‑9 in Human Ovarian Cancer Tissue. View Larger

MMP‑9 in Human Ovarian Cancer Tissue. MMP‑9 was detected in immersion fixed paraffin-embedded sections of human ovarian cancer tissue using Human MMP‑9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF911) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MMP-9

Pro-MMP-9 is an inactive proenzyme secreted by multiple cell types. The N-terminal pro region is proteolytically removed, resulting in active MMP-9 which can degrade collagens and elastin as well as several non-ECM molecules.

Long Name
Matrix Metalloproteinase 9
Entrez Gene IDs
4318 (Human); 17395 (Mouse); 81687 (Rat)
Alternate Names
92 kDa gelatinase; 92 kDa type IV collagenase; CLG4B; EC 3.4.24; EC 3.4.24.35; Gelatinase B; GELB; macrophage gelatinase; MANDP2; matrix metallopeptidase 9; matrix metalloproteinase 9; matrix metalloproteinase-9; MMP9; MMP-9; type V collagenase

Product Datasheets

Citations for Human MMP-9 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

11 Citations: Showing 1 - 10
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  1. EDTA/gelatin zymography method to identify C1s versus activated MMP-9 in plasma and immune complexes of patients with systemic lupus erythematosus
    Authors: E Ugarte-Ber, E Martens, L Boon, J Vandooren, D Blockmans, P Proost, G Opdenakker
    J. Cell. Mol. Med., 2019;23(1):576-585.
  2. RAGE mediates S100A7-induced breast cancer growth and metastasis by modulating the tumor microenvironment.
    Authors: Nasser M, Wani N, Ahirwar D, Powell C, Ravi J, Elbaz M, Zhao H, Padilla L, Zhang X, Shilo K, Ostrowski M, Shapiro C, Carson W, Ganju R
    Cancer Res, 2015;75(6):974-85.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  3. Plasma levels of the MMP-9:TIMP-1 complex as prognostic biomarker in breast cancer: a retrospective study.
    Authors: Thorsen S, Christensen S, Wurtz S, Lundberg M, Nielsen B, Vinther L, Knowles M, Gee N, Fredriksson S, Moller S, Brunner N, Schrohl A, Stenvang J
    BMC Cancer, 2013;13(0):598.
    Species: Human
    Sample Types: Plasma
    Applications: Proximity Ligation Assay (PLA)
  4. Humanin, a cytoprotective peptide, is expressed in carotid atherosclerotic [corrected] plaques in humans.
    Authors: Zacharias DG, Kim SG, Massat AE, Bachar AR, Oh YK, Herrmann J, Rodriguez-Porcel M, Cohen P, Lerman LO, Lerman A
    PLoS ONE, 2012;7(2):e31065.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  5. GDF-15 is an inhibitor of leukocyte integrin activation required for survival after myocardial infarction in mice.
    Authors: Kempf T, Zarbock A, Widera C, Butz S, Stadtmann A, Rossaint J, Bolomini-Vittori M, Korf-Klingebiel M, Napp LC, Hansen B, Kanwischer A, Bavendiek U, Beutel G, Hapke M, Sauer MG, Laudanna C, Hogg N, Vestweber D, Wollert KC
    Nat. Med., 2011;17(5):581-8.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  6. TRE17/USP6 oncogene translocated in aneurysmal bone cyst induces matrix metalloproteinase production via activation of NF-kappaB.
    Authors: Ye Y, Pringle LM, Lau AW
    Oncogene, 2010;29(25):3619-29.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  7. Fibroblast-conditioned media promote human sarcoma cell invasion.
    Authors: Bittner JG, Wilson M, Shah MB, Albo D, Feig BW, Wang TN
    Surgery, 2009;145(1):42-7.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  8. Development and validation of sandwich ELISA microarrays with minimal assay interference.
    Authors: Gonzalez RM, Seurynck-Servoss SL, Crowley SA
    J. Proteome Res., 2008;7(6):2406-14.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Microarray Development
  9. Molecular profiling of cervical cancer progression.
    Authors: Hagemann T, Bozanovic T, Hooper S, Ljubic A, Slettenaar VI, Wilson JL, Singh N, Gayther SA, Shepherd JH, Van Trappen PO
    Br. J. Cancer, 2007;96(2):321-8.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  10. Differential proteolytic enzyme activity in eosinophilic and neutrophilic asthma.
    Authors: Simpson JL, Scott RJ, Boyle MJ, Gibson PG
    Am. J. Respir. Crit. Care Med., 2005;172(5):559-65.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  11. Differential expression and activity of matrix metalloproteinases during flow-modulated vein graft remodeling.
    Authors: Berceli SA, Jiang Z, Klingman NV, Pfahnl CL, Abouhamze ZS, Frase CD, Schultz GS, Ozaki CK
    J. Vasc. Surg., 2004;39(5):1084-90.
    Species: Rabbit
    Sample Types: Whole Tissue
    Applications: IHC-P

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Secondary Antibodies

Reviews for Human MMP-9 Antibody

Average Rating: 4.2 (Based on 5 Reviews)

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Human MMP-9 Antibody
By Anonymous on 07/13/2018
Application: WB Sample Tested: HeLa cells Species: Human

Human MMP-9 Antibody
By Anonymous on 07/02/2018
Application: WB Sample Tested: Purified MMP9,Purified Human MMP9 Species: Human

Human MMP-9 Antibody
By Anonymous on 12/06/2017
Application: ELISA Sample Tested: EDTA Plasma Species: Human

Human MMP-9 Antibody
By Anonymous on 11/10/2017
Application: ELISA Sample Tested: Serum and Plasma Species: Human

This antibody was used as the detection in an ELISA built to measure MMP-9 in human serum samples. MAB936 was used as the capture antibody in the immunoassay.


Human MMP‑9 Antibody
By Anonymous on 02/27/2016
Application: WB Sample Tested: Human cell conditioned medium Species: Human