Human MMP-9 Antibody
Human MMP-9 Antibody Summary
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Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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MMP‑9 in NS0 Mouse Cell Line. MMP‑9 was detected in immersion fixed NS0 mouse myeloma cell line transfected with MMP-9 using 5 µg/mL Human MMP‑9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF911) for 3 hours at room temperature. Cells were stained (red) and counterstained (green). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
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MMP‑9 in Human Ovarian Cancer Tissue. MMP-9 was detected in immersion fixed paraffin-embedded sections of human ovarian cancer tissue using Human MMP-9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF911) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
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MMP‑9 in MCF‑7 Human Cell Line. MMP-9 was detected in immersion fixed MCF-7 human breast cancer cell line using Human MMP-9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF911) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
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MMP‑9 in Human Ovarian Cancer Tissue. MMP-9 was detected in immersion fixed paraffin-embedded sections of human ovarian cancer tissue using Human MMP-9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF911) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: MMP-9
Pro-MMP-9 is an inactive proenzyme secreted by multiple cell types. The N-terminal pro region is proteolytically removed, resulting in active MMP-9 which can degrade collagens and elastin as well as several non-ECM molecules.
Product Datasheets
Citations for Human MMP-9 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
14
Citations: Showing 1 - 10
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ADAM8-Dependent Extracellular Signaling in the Tumor Microenvironment Involves Regulated Release of Lipocalin 2 and MMP-9
Authors: L Cook, M Sengelmann, B Winkler, C Nagl, S Koch, U Schlomann, EP Slater, MA Miller, EP von Strand, B Dörsam, C Preu beta er, JW Bartsch
International Journal of Molecular Sciences, 2022;23(4):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Ubiquitin-specific protease TRE17/USP6 promotes tumor cell invasion through the regulation of glycoprotein CD147 intracellular trafficking
Authors: Y Ogura, N Ohbayashi, Y Kanaho, A Kawaguchi, Y Funakoshi
The Journal of Biological Chemistry, 2022;0(0):102335.
Species: Human
Sample Types: Cell Lysates
Applications: Zymography -
CD44 modulates metabolic pathways and altered ROS-mediated Akt signal promoting cholangiocarcinoma progression
Authors: M Thanee, H Dokduang, Y Kittirat, J Phetcharab, P Klanrit, A Titapun, N Namwat, N Khuntikeo, A Wangwiwats, H Saya, W Loilome
PLoS ONE, 2021;16(3):e0245871.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
EDTA/gelatin zymography method to identify C1s versus activated MMP-9 in plasma and immune complexes of patients with systemic lupus erythematosus
Authors: E Ugarte-Ber, E Martens, L Boon, J Vandooren, D Blockmans, P Proost, G Opdenakker
J. Cell. Mol. Med., 2019;23(1):576-585.
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RAGE mediates S100A7-induced breast cancer growth and metastasis by modulating the tumor microenvironment.
Authors: Nasser M, Wani N, Ahirwar D, Powell C, Ravi J, Elbaz M, Zhao H, Padilla L, Zhang X, Shilo K, Ostrowski M, Shapiro C, Carson W, Ganju R
Cancer Res, 2015;75(6):974-85.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Plasma levels of the MMP-9:TIMP-1 complex as prognostic biomarker in breast cancer: a retrospective study.
Authors: Thorsen S, Christensen S, Wurtz S, Lundberg M, Nielsen B, Vinther L, Knowles M, Gee N, Fredriksson S, Moller S, Brunner N, Schrohl A, Stenvang J
BMC Cancer, 2013;13(0):598.
Species: Human
Sample Types: Plasma
Applications: Proximity Ligation Assay (PLA) -
Humanin, a cytoprotective peptide, is expressed in carotid atherosclerotic [corrected] plaques in humans.
Authors: Zacharias DG, Kim SG, Massat AE, Bachar AR, Oh YK, Herrmann J, Rodriguez-Porcel M, Cohen P, Lerman LO, Lerman A
PLoS ONE, 2012;7(2):e31065.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
GDF-15 is an inhibitor of leukocyte integrin activation required for survival after myocardial infarction in mice.
Authors: Kempf T, Zarbock A, Widera C, Butz S, Stadtmann A, Rossaint J, Bolomini-Vittori M, Korf-Klingebiel M, Napp LC, Hansen B, Kanwischer A, Bavendiek U, Beutel G, Hapke M, Sauer MG, Laudanna C, Hogg N, Vestweber D, Wollert KC
Nat. Med., 2011;17(5):581-8.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC -
TRE17/USP6 oncogene translocated in aneurysmal bone cyst induces matrix metalloproteinase production via activation of NF-kappaB.
Authors: Ye Y, Pringle LM, Lau AW
Oncogene, 2010;29(25):3619-29.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Fibroblast-conditioned media promote human sarcoma cell invasion.
Authors: Bittner JG, Wilson M, Shah MB, Albo D, Feig BW, Wang TN
Surgery, 2009;145(1):42-7.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Development and validation of sandwich ELISA microarrays with minimal assay interference.
Authors: Gonzalez RM, Seurynck-Servoss SL, Crowley SA
J. Proteome Res., 2008;7(6):2406-14.
Species: Human
Sample Types: Serum
Applications: ELISA Microarray Development -
Molecular profiling of cervical cancer progression.
Authors: Hagemann T, Bozanovic T, Hooper S, Ljubic A, Slettenaar VI, Wilson JL, Singh N, Gayther SA, Shepherd JH, Van Trappen PO
Br. J. Cancer, 2007;96(2):321-8.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P -
Differential proteolytic enzyme activity in eosinophilic and neutrophilic asthma.
Authors: Simpson JL, Scott RJ, Boyle MJ, Gibson PG
Am. J. Respir. Crit. Care Med., 2005;172(5):559-65.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Differential expression and activity of matrix metalloproteinases during flow-modulated vein graft remodeling.
Authors: Berceli SA, Jiang Z, Klingman NV, Pfahnl CL, Abouhamze ZS, Frase CD, Schultz GS, Ozaki CK
J. Vasc. Surg., 2004;39(5):1084-90.
Species: Rabbit
Sample Types: Whole Tissue
Applications: IHC-P
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This antibody was used as the detection in an ELISA built to measure MMP-9 in human serum samples. MAB936 was used as the capture antibody in the immunoassay.
