Human/Mouse Active Caspase-3 Antibody

(113 citations)
(1 Review)
  
  • Species Reactivity
    Human, Mouse
  • Specificity
    Distinguishes apoptotic cells from non-apoptotic cells in immunohistochemistry. Detects the p17 subunit of Caspase-3, but does not detect the precursor form in Western blot. We recommend R&D Systems Affinity Purified Anti-Caspase-3 (Catalog # AF-605-NA) or Monoclonal Anti-Cleaved-Caspase-3 (Catalog # MAB835) for Western blot applications.
  • Source
    Polyclonal Rabbit IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    KLH-coupled human Caspase-3 synthetic peptide
    CRGTELDCGIETD
    Accession # P42574
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Immunocytochemistry
    5-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunocytochemistry
Caspase‑3 in Jurkat Human Cell Line. Caspase‑3 was detected in immersion fixed anti-FAS treated Jurkat human acute T cell leukemia cell line using 0.3 µg/mL Human/Mouse Active Caspase‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF835) for 3 hours at room temperature. Cells were stained (red) and counterstained (green). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Immunocytochemistry
Caspase‑3 in Jurkat Human Cell Line. Caspase‑3 was detected in immersion fixed Jurkat human acute T cell leukemia cell line stimulated with staurosporin using Human/Mouse Active Caspase‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF835) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (yellow; Catalog # NL004) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Caspase-3

Caspases are a family of cytosolic aspartate-specific cysteine proteases involved in the initiation and execution of apoptosis. They are expressed as latent zymogens and are activated by an autoproteolytic mechanism or by processing by other proteases (frequently other caspases). Human caspases can be subdivided into three functional groups: cytokine activation (caspase-1, -4, -5, and -13), apoptosis initiation (caspase-2, -8, -9, -and -10), and apoptosis execution (caspase-3, -6, and -7).

Caspases are regulated by a variety of stimili, including APAF1, CFLAR/FLIP, NOL3/ARC, and members of the inhibitor of apoptosis (IAP) family such as BIRC1/NAIP, BIRC2/cIAP-1, BIRC3/cIAP-2, BIRC4/XIAP, BIRC5/Survivin, and BIRC7/Livin. IAP activity is modulated by DIABLO/SMAC or PRSS25/HTRA2/Omi. Cell-permeable and irreversible peptide inhibitors are also available for different caspases.

  • Entrez Gene IDs:
    836 (Human); 12367 (Mouse)
  • Alternate Names:
    Apopain; apoptosis-related cysteine protease; CASP3; CASP-3; caspase 3, apoptosis-related cysteine peptidase; Caspase3; Caspase-3; CPP32; CPP-32; CPP32B; CPP32SREBP cleavage activity 1; Cysteine protease CPP32; EC 3.4.22; EC 3.4.22.56; LICE-1; PARP cleavage protease; procaspase3; Protein Yama; SCA-1; YAMA
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

113 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. The scaffold protein p140Cap limits ERBB2-mediated breast cancer progression interfering with Rac GTPase-controlled circuitries
    Authors: S Grasso, J Chapelle, V Salemme, S Aramu, I Russo, N Vitale, L Verdun di, K Dallaglio, I Castellano, A Amici, G Centonze, N Sharma, S Lunardi, S Cabodi, F Cavallo, A Lamolinara, L Stramucci, E Moiso, P Provero, A Albini, A Sapino, J Staaf, PP Di Fiore, G Bertalot, S Pece, D Tosoni, S Confalonie, M Iezzi, P Di Stefano, E Turco, P Defilippi
    Nat Commun, 2017;8(0):14797.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  2. Low Dose Radiation Causes Skin Cancer In Mice And Has A Differential Effect on Distinct Epidermal Stem Cells
    Authors: T Revenco, G Lapouge, V Moers, S Broh‚e, PA Sotiropoul
    Stem Cells, 2017;0(0):.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC - Paraffin embedded
  3. Bifidobacterium breve reduces apoptotic epithelial cell shedding in an exopolysaccharide and MyD88-dependent manner
    Authors: KR Hughes, LC Harnisch, C Alcon-Gine, S Mitra, CJ Wright, J Ketskemety, D van Sinder, AJ Watson, LJ Hall
    Open Biol, 2017;7(1):.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC - Paraffin embedded
  4. Combined effects of atorvastatin and aspirin on growth and apoptosis in human prostate cancer cells
    Authors: Y He, H Huang, C Farischon, D Li, Z Du, K Zhang, X Zheng, S Goodin
    Oncol. Rep, 2017;37(2):953-960.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  5. VEGFR2 but not VEGFR3 governs integrity and remodeling of thyroid angiofollicular unit in normal state and during goitrogenesis
    Authors: JY Jang, SY Choi, I Park, DY Park, K Choe, P Kim, YK Kim, BJ Lee, M Hirashima, Y Kubota, JW Park, SY Cheng, A Nagy, YJ Park, K Alitalo, M Shong, GY Koh
    EMBO Mol Med, 2017;0(0):.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  6. Combined toll-like receptor 3/7/9 deficiency on host cells results in T-cell-dependent control of tumour growth
    Authors: JC Klein, K Moses, G Zelinskyy, S Sody, J Buer, S Lang, I Helfrich, U Dittmer, CJ Kirschning, S Brandau
    Nat Commun, 2017;8(0):14600.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  7. New tumor suppressor microRNAs target glypican-3 in human liver cancer
    Authors: F Cartier, E Indersie, S Lesjean, J Charpentie, KB Hooks, A Ghousein, A Desplat, N Dugot-Sena, V Trézéguet, F Sagliocco, M Hagedorn, CF Grosset
    Oncotarget, 2017;0(0):.
    Species: Chicken
    Sample Type: Whole Tissue
    Application: IHC
  8. Loss of the Wnt receptor Frizzled7 in the gastric epithelium is deleterious and triggers rapid repopulation in vivo
    Authors: DJ Flanagan, N Barker, C Nowell, H Clevers, M Ernst, TJ Phesse, E Vincan
    Dis Model Mech, 2017;0(0):.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  9. Acute cigarette smoke exposure activates apoptotic and inflammatory programs but a second stimulus is required to induce epithelial to mesenchymal transition in COPD epithelium
    Authors: LA Murray, R Dunmore, A Camelo, CA Da Silva, MJ Gustavsson, DM Habiel, TL Hackett, CM Hogaboam, MA Sleeman, DA Knight
    Respir. Res., 2017;18(1):82.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Not Specified
  10. A role for core planar polarity proteins in cell contact-mediated orientation of planar cell division across the mammalian embryonic skin
    Authors: F Oozeer, LL Yates, C Dean, CJ Formstone
    Sci Rep, 2017;7(1):1880.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  11. Glycogen synthase kinase-3 inhibition sensitizes human induced pluripotent stem cells to thiol-containing antioxidants induced apoptosis
    Authors: C Tu, R Xu, M Koleti, J Zoldan
    Stem Cell Res, 2017;23(0):182-187.
    Species: Human
    Sample Type: Whole Cells
    Application: Functional Assay
  12. Mutations in Cockayne Syndrome-Associated Genes (Csa and Csb) Predispose to Cisplatin-Induced Hearing Loss in Mice
    J Neurosci, 2016;36(17):4758-70.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Not Specified
  13. Amplification of R-spondin1 signaling induces granulosa cell fate defects and cancers in mouse adult ovary
    Authors: M-C De Cian
    Oncogene, 2016;0(0):.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC - Paraffin embedded
  14. Analysis of Tyrosine Kinase Inhibitor-Mediated Decline in Contractile Force in Rat Engineered Heart Tissue.
    Authors: Jacob F, Yonis A, Cuello F, Luther P, Schulze T, Eder A, Streichert T, Mannhardt I, Hirt M, Schaaf S, Stenzig J, Force T, Eschenhagen T, Hansen A
    PLoS ONE, 2016;11(2):e0145937.
    Species: Rat
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  15. Fbxw7-associated drug resistance is reversed by induction of terminal differentiation in murine intestinal organoid culture
    Authors: F Lorenzi, R Babaei-Jad, J Sheard, B Spencer-De, AS Nateri
    Mol Ther Methods Clin Dev, 2016;3(0):16024.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  16. CDK7 and miR-210 Co-regulate Cell-Cycle Progression of Neural Progenitors in the Developing Neocortex
    Stem Cell Reports, 2016;7(1):69-79.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC - PFA fixed
  17. Brinp1 (-/-) mice exhibit autism-like behaviour, altered memory, hyperactivity and increased parvalbumin-positive cortical interneuron density
    Authors: SR Berkowicz, TJ Featherby, Z Qu, A Giousoh, NA Borg, JI Heng, JC Whisstock, PI Bird
    Mol Autism, 2016;7(0):22.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC - Frozen
  18. Activation of FADD-Dependent Neuronal Death Pathways as a Predictor of Pathogenicity for LRRK2 Mutations
    PLoS ONE, 2016;11(11):e0166053.
    Species: Human
    Sample Type: Whole Cells
    Application: IHC
  19. Autophagy deficient keratinocytes display increased DNA damage, senescence and aberrant lipid composition after oxidative stress in vitro and in vivo
    Redox Biol, 2016;11(0):219-230.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  20. cMyc Regulates the Size of the Premigratory Neural Crest Stem Cell Pool
    Cell Rep, 2016;17(10):2648-2659.
    Species: Mouse
    Sample Type: Whole Cells
    Application: IHC
  21. Defining the cellular lineage hierarchy in the interfollicular epidermis of adult skin
    Nat. Cell Biol., 2016;18(6):619-31.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC OCT-embedded
  22. Pulmonary alveolar macrophages contribute to the premetastatic niche by suppressing antitumor T cell responses in the lungs.
    Authors: Sharma S, Chintala N, Vadrevu S, Patel J, Karbowniczek M, Markiewski M
    J Immunol, 2015;194(11):5529-38.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  23. Apoptosis-Related Factors in the Luteal Phase of the Domestic Cat and Their Involvement in the Persistence of Corpora Lutea in Lynx.
    Authors: Amelkina O, Zschockelt L, Painer J, Serra R, Villaespesa F, Braun B, Jewgenow K
    PLoS ONE, 2015;10(11):e0143414.
    Species: Feline
    Sample Type: Whole Tissue
    Application: IHC - paraffin
  24. Exacerbated experimental colitis in TNFAIP8-deficient mice.
    Authors: Sun H, Lou Y, Porturas T, Morrissey S, Luo G, Qi J, Ruan Q, Shi S, Chen Y
    J Immunol, 2015;194(12):5736-42.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  25. Deficiency for the cysteine protease cathepsin L impairs Myc-induced tumorigenesis in a mouse model of pancreatic neuroendocrine cancer.
    Authors: Brindle N, Joyce J, Rostker F, Lawlor E, Swigart-Brown L, Evan G, Hanahan D, Shchors K
    PLoS ONE, 2015;10(4):e0120348.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC-OCT embedded
  26. Epidermal growth factor attenuates tubular necrosis following mercuric chloride damage by regeneration of indigenous, not bone marrow-derived cells.
    Authors: Yen T, Alison M, Goodlad R, Otto W, Jeffery R, Cook H, Wright N, Poulsom R
    J Cell Mol Med, 2015;19(2):463-73.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC - paraffin
  27. Preclinical studies identify non-apoptotic low-level caspase-3 as therapeutic target in pemphigus vulgaris.
    Authors: Luyet C, Schulze K, Sayar B, Howald D, Muller E, Galichet A
    PLoS ONE, 2015;10(3):e0119809.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC Fluorescence
  28. Attenuation of Cerebral Ischemic Injury in Smad1 Deficient Mice.
    Authors: Wong J, Chen L, Huang Y, Sehba F, Friedel R, Zou H
    PLoS ONE, 2015;10(8):e0136967.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  29. Metformin inhibits growth of human glioblastoma cells and enhances therapeutic response.
    Authors: Sesen J, Dahan P, Scotland S, Saland E, Dang V, Lemarie A, Tyler B, Brem H, Toulas C, Cohen-Jonathan Moyal E, Sarry J, Skuli N
    PLoS ONE, 2015;10(4):e0123721.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC - Not specified
  30. An essential role for Ink4 and Cip/Kip cell-cycle inhibitors in preventing replicative stress.
    Authors: Quereda V, Porlan E, Canamero M, Dubus P, Malumbres M
    Cell Death Differ, 2015;23(3):430-41.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  31. The Inflammatory Caspases-1 and -11 Mediate the Pathogenesis of Dermatitis in Sharpin-Deficient Mice.
    Authors: Douglas T, Champagne C, Morizot A, Lapointe J, Saleh M
    J Immunol, 2015;195(5):2365-73.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC - Paraffin embedded
  32. Alpha-enolase is upregulated on the cell surface and responds to plasminogen activation in mice expressing a 133p53alpha mimic.
    Authors: Sawhney S, Hood K, Shaw A, Braithwaite A, Stubbs R, Hung N, Royds J, Slatter T
    PLoS ONE, 2015;10(2):e0116270.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  33. Interfering with stem cell-specific gatekeeper functions controls tumour initiation and malignant progression of skin tumours.
    Authors: Petersson, Monika, Reuter, Karen, Brylka, Heike, Kraus, Andreas, Schettina, Peter, Niemann, Catherin
    Nat Commun, 2015;6(0):5874.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Not Specified
  34. Demyelination causes adult CNS progenitors to revert to an immature state and express immune cues that support their migration.
    Authors: Moyon S, Dubessy A, Aigrot M, Trotter M, Huang J, Dauphinot L, Potier M, Kerninon C, Melik Parsadaniantz S, Franklin R, Lubetzki C
    J Neurosci, 2015;35(1):4-20.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  35. Hepatocyte-specific deletion of ARNT (aryl hydrocarbon Receptor Nuclear Translocator) results in altered fibrotic gene expression in the thioacetamide model of liver injury.
    Authors: Scott C, Cha K, Rao R, Liddle C, George J, Gunton J
    PLoS ONE, 2015;10(3):e0121650.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  36. BMP9 and BMP10 are necessary for proper closure of the ductus arteriosus.
    Authors: Levet S, Ouarne M, Ciais D, Coutton C, Subileau M, Mallet C, Ricard N, Bidart M, Debillon T, Faravelli F, Rooryck C, Feige J, Tillet E, Bailly S
    Proc Natl Acad Sci U S A, 2015;112(25):E3207-15.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Not Specified
  37. Interactions of highly and low virulent Flavobacterium columnare isolates with gill tissue in carp and rainbow trout.
    Authors: Declercq, Annelies, Chiers, Koen, Van den Broeck, Wim, Dewulf, Jeroen, Eeckhaut, Venessa, Cornelissen, Maria, Bossier, Peter, Haesebrouck, Freddy, Decostere, Annemie
    Vet Res, 2015;46(0):25.
    Species: Fish - Cyprinus carpio (Carp)
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  38. TLR9 signalling in microglia attenuates seizure-induced aberrant neurogenesis in the adult hippocampus.
    Authors: Matsuda T, Murao N, Katano Y, Juliandi B, Kohyama J, Akira S, Kawai T, Nakashima K
    Nat Commun, 2015;6(0):6514.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC Fluorescence
  39. Biphasic influence of Miz1 on neural crest development by regulating cell survival and apical adhesion complex formation in the developing neural tube.
    Authors: Kerosuo L, Bronner M
    Mol Biol Cell, 2014;25(3):347-55.
    Species: Chicken
    Sample Type: Whole Tissue
    Application: IHC
  40. Oncogenic mutations in intestinal adenomas regulate Bim-mediated apoptosis induced by TGF-beta.
    Authors: Wiener Z, Band A, Kallio P, Hogstrom J, Hyvonen V, Kaijalainen S, Ritvos O, Haglund C, Kruuna O, Robine S, Louvard D, Ben-Neriah Y, Alitalo K
    Proc Natl Acad Sci U S A, 2014;111(21):E2229-36.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: Flow
  41. Crim1 maintains retinal vascular stability during development by regulating endothelial cell Vegfa autocrine signaling.
    Authors: Fan J, Ponferrada V, Sato T, Vemaraju S, Fruttiger M, Gerhardt H, Ferrara N, Lang R
    Development, 2014;141(2):448-59.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Not Specified
  42. The extracellular matrix glycoprotein tenascin-R regulates neurogenesis during development and in the adult dentate gyrus of mice.
    Authors: Xu J, Xiao M, Jakovcevski I, Sivukhina E, Hargus G, Cui Y, Irintchev A, Schachner M, Bernreuther C
    J Cell Sci, 2014;127(0):641-52.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  43. Endoplasmic reticulum stress in amelogenesis imperfecta and phenotypic rescue using 4-phenylbutyrate.
    Authors: Brookes, Steven J, Barron, Martin J, Boot-Handford, Ray, Kirkham, Jennifer, Dixon, Michael
    Hum Mol Genet, 2014;23(9):2468-80.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  44. Foxi transcription factors promote pharyngeal arch development by regulating formation of FGF signaling centers.
    Authors: Edlund R, Ohyama T, Kantarci H, Riley B, Groves A
    Dev Biol, 2014;390(1):1-13.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  45. Dysregulated STAT1-SOCS1 control of JAK2 promotes mammary luminal progenitor cell survival and drives ERalpha(+) tumorigenesis.
    Authors: Chan S, Rickert C, Vermi W, Sheehan K, Arthur C, Allen J, White J, Archambault J, Lonardi S, McDevitt T, Bhattacharya D, Lorenzi M, Allred D, Schreiber R
    Cell Death Differ, 2014;21(2):234-46.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  46. Essential role for the TRF2 telomere protein in adult skin homeostasis.
    Authors: Martinez P, Ferrara-Romeo I, Flores J, Blasco M
    Aging Cell, 2014;13(4):656-68.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  47. Germline quality control: eEF2K stands guard to eliminate defective oocytes.
    Authors: Chu H, Liao Y, Novak J, Hu Z, Merkin J, Shymkiv Y, Braeckman B, Dorovkov M, Nguyen A, Clifford P, Nagele R, Harrison D, Ellis R, Ryazanov A
    Dev Cell, 2014;28(5):561-72.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  48. mTORC1 maintains renal tubular homeostasis and is essential in response to ischemic stress.
    Authors: Grahammer F, Haenisch N, Steinhardt F, Sander L, Roerden M, Arnold F, Cordts T, Wanner N, Reichardt W, Kerjaschki D, Ruegg M, Hall M, Moulin P, Busch H, Boerries M, Walz G, Artunc F, Huber T
    Proc Natl Acad Sci U S A, 2014;111(27):E2817-26.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  49. Lymphatic regulator PROX1 determines Schlemm's canal integrity and identity.
    Authors: Park D, Lee J, Park I, Choi D, Lee S, Song S, Hwang Y, Hong K, Nakaoka Y, Makinen T, Kim P, Alitalo K, Hong Y, Koh G
    J Clin Invest, 2014;124(9):3960-74.
  50. Erythropoietin suppresses the activation of pro-apoptotic genes in head and neck squamous cell carcinoma xenografts exposed to surgical trauma.
    Authors: Lindgren G, Ekblad L, Vallon-Christersson J, Kjellen E, Gebre-Medhin M, Wennerberg J
    BMC Cancer, 2014;14(0):648.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Not Specified
  51. Pseudomonas aeruginosa-induced apoptosis in airway epithelial cells is mediated by gap junctional communication in a JNK-dependent manner.
    Authors: Losa D, Kohler T, Bellec J, Dudez T, Crespin S, Bacchetta M, Boulanger P, Hong S, Morel S, Nguyen T, van Delden C, Chanson M
    J Immunol, 2014;192(10):4804-12.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  52. Differential sensitivity of prefrontal cortex and hippocampus to alcohol-induced toxicity.
    Authors: Fowler, Anna-Kat, Thompson, Jeremy, Chen, Lixia, Dagda, Marisela, Dertien, Janet, Dossou, Katina S, Moaddel, Ruin, Bergeson, Susan E, Kruman, Inna I
    PLoS ONE, 2014;9(9):e106945.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  53. Leucine-rich repeat containing 8A (LRRC8A) is essential for T lymphocyte development and function.
    Authors: Kumar L, Chou J, Yee C, Borzutzky A, Vollmann E, von Andrian U, Park S, Hollander G, Manis J, Poliani P, Geha R
    J Exp Med, 2014;211(5):929-42.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  54. Short term morphine exposure in vitro alters proliferation and differentiation of neural progenitor cells and promotes apoptosis via mu receptors.
    Authors: Willner D, Cohen-Yeshurun A, Avidan A, Ozersky V, Shohami E, Leker R
    PLoS ONE, 2014;9(7):e103043.
    Species: Mouse
    Sample Type: Whole Cells
    Application: IHC
  55. LMO4 functions as a negative regulator of sensory organ formation in the mammalian cochlea.
    Authors: Deng M, Luo X, Pan L, Yang H, Xie X, Liang G, Huang L, Hu F, Kiernan A, Gan L
    J Neurosci, 2014;34(30):10072-7.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  56. Antigen dependently activated cluster of differentiation 8-positive T cells cause perforin-mediated neurotoxicity in experimental stroke.
    Authors: Mracsko E, Liesz A, Stojanovic A, Lou W, Osswald M, Zhou W, Karcher S, Winkler F, Martin-Villalba A, Cerwenka A, Veltkamp R
    J Neurosci, 2014;34(50):16784-95.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC Fluorescence
  57. Caspase 3 involves in neuroplasticity, microglial activation and neurogenesis in the mice hippocampus after intracerebral injection of kainic acid.
    Authors: Tzeng, Tsai-Ten, Tsay, Huey-Jen, Chang, Luping, Hsu, Chia-Lin, Lai, Tzu-Hsua, Huang, Fong-Lee, Shiao, Young-Ji
    J Biomed Sci, 2013;20(0):90.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Fresh
  58. Requirements for Aurora-A in tissue regeneration and tumor development in adult mammals.
    Authors: Perez de Castro I, Aguirre-Portoles C, Fernandez-Miranda G, Canamero M, Cowley D, Van Dyke T, Malumbres M
    Cancer Res, 2013;73(22):6804-15.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  59. Mechanism of cystogenesis in nephrotic kidneys: a histopathological study.
    Authors: Saraga M, Vukojevic K, Krzelj V, Puretic Z, Bocina I, Durdov M, Weber S, Dworniczak B, Ljubanovic D, Saraga-Babic M
    BMC Nephrol, 2013;15(0):3.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC - Not specified
  60. The temporal and spatial profiles of cell loss following experimental spinal cord injury: effect of antioxidant therapy on cell death and functional recovery.
    Authors: Ling, Xiang, Bao, Feng, Qian, Hao, Liu, Danxia
    BMC Neurosci, 2013;14(0):146.
    Species: Rat
    Sample Type: Whole Tissue
    Application: IHC
  61. Curcumin enhances the effect of chemotherapy against colorectal cancer cells by inhibition of NF-kappaB and Src protein kinase signaling pathways.
    Authors: Shakibaei M, Mobasheri A, Lueders C, Busch F, Shayan P, Goel A
    PLoS ONE, 2013;8(2):e57218.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  62. A component of the mir-17-92 polycistronic oncomir promotes oncogene-dependent apoptosis.
    Authors: Olive V, Sabio E, Bennett M, De Jong C, Biton A, McGann J, Greaney S, Sodir N, Zhou A, Balakrishnan A, Foth M, Luftig M, Goga A, Speed T, Xuan Z, Evan G, Wan Y, Minella A, He L
    Elife, 2013;2(0):e00822.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  63. Epigenetics and cell death: DNA hypermethylation in programmed retinal cell death.
    Authors: Wahlin, Karl J, Enke, Raymond, Fuller, John A, Kalesnykas, Giedrius, Zack, Donald J, Merbs, Shannath
    PLoS ONE, 2013;8(11):e79140.
    Species: Chicken
    Sample Type: Whole Tissue
    Application: IHC Frozen
  64. Foxa1 and foxa2 are required for the maintenance of dopaminergic properties in ventral midbrain neurons at late embryonic stages.
    Authors: Stott, Simon R, Metzakopian, Emmanoui, Lin, Wei, Kaestner, Klaus H, Hen, Rene, Ang, Siew-Lan
    J Neurosci, 2013;33(18):8022-34.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC OCT-embedded
  65. Diet-induced obesity exacerbates auditory degeneration via hypoxia, inflammation, and apoptosis signaling pathways in CD/1 mice.
    Authors: Hwang J, Hsu C, Yu W, Liu T, Yang W
    PLoS ONE, 2013;8(4):e60730.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  66. Increased chondrocyte apoptosis is associated with progression of osteoarthritis in spontaneous Guinea pig models of the disease.
    Authors: Zamli Z, Adams M, Tarlton J, Sharif M
    Int J Mol Sci, 2013;14(9):17729-43.
    Species: Guinea Pig
    Sample Type: Whole Tissue
    Application: IHC
  67. Role of Gadd45a in Wip1-dependent regulation of intestinal tumorigenesis.
    Cell Death Differ., 2012;19(11):1761-8.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  68. Carbonic anhydrase IX promotes tumor growth and necrosis in vivo and inhibition enhances anti-VEGF therapy.
    Authors: McIntyre A, Patiar S, Wigfield S
    Clin. Cancer Res., 2012;18(11):3100-11.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC
  69. An RNA interference screen uncovers a new molecule in stem cell self-renewal and long-term regeneration.
    Authors: Chen T, Heller E, Beronja S, Oshimori N, Stokes N, Fuchs E
    Nature, 2012;485(7396):104-8.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  70. Pericyte requirement for anti-leak action of angiopoietin-1 and vascular remodeling in sustained inflammation.
    Authors: Fuxe J, Tabruyn S, Colton K, Zaid H, Adams A, Baluk P, Lashnits E, Morisada T, Le T, O'Brien S, Epstein DM, Koh GY, McDonald DM
    Am. J. Pathol., 2011;178(6):2897-909.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  71. Robo1 regulates semaphorin signaling to guide the migration of cortical interneurons through the ventral forebrain.
    Authors: Hernandez-Miranda LR, Cariboni A, Faux C, Ruhrberg C, Cho JH, Cloutier JF, Eickholt BJ, Parnavelas JG, Andrews WD
    J. Neurosci., 2011;31(16):6174-87.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  72. Bcl-2 and accelerated DNA repair mediates resistance of hair follicle bulge stem cells to DNA-damage-induced cell death.
    Authors: Sotiropoulou PA, Candi A, Mascre G, De Clercq S, Youssef KK, Lapouge G, Dahl E, Semeraro C, Denecker G, Marine JC, Blanpain C
    Nat. Cell Biol., 2010;12(6):572-82.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  73. Two hypomorphic alleles of mouse Ass1 as a new animal model of citrullinemia type I and other hyperammonemic syndromes.
    Authors: Perez CJ, Jaubert J, Guenet JL, Barnhart KF, Ross-Inta CM, Quintanilla VC, Aubin I, Brandon JL, Otto NW, DiGiovanni J, Gimenez-Conti I, Giulivi C, Kusewitt DF, Conti CJ, Benavides F
    Am. J. Pathol., 2010;177(4):1958-68.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  74. Complementary actions of inhibitors of angiopoietin-2 and VEGF on tumor angiogenesis and growth.
    Authors: Hashizume H, Falcon BL, Kuroda T, Baluk P, Coxon A, Yu D, Bready JV, Oliner JD, McDonald DM
    Cancer Res., 2010;70(6):2213-23.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC
  75. ALK1 signaling regulates early postnatal lymphatic vessel development.
    Authors: Niessen K, Zhang G, Ridgway JB, Chen H, Yan M
    Blood, 2010;115(8):1654-61.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  76. MicroRNA-183 family members regulate sensorineural fates in the inner ear.
    Authors: Li H, Kloosterman W, Fekete DM
    J. Neurosci., 2010;30(9):3254-63.
    Species: Zebrafish
    Sample Type: Whole Tissue
    Application: IHC
  77. Sustained CTL activation by murine pulmonary epithelial cells promotes the development of COPD-like disease.
    Authors: Borchers MT, Wesselkamper SC, Curull V, Ramirez-Sarmiento A, Sanchez-Font A, Garcia-Aymerich J, Coronell C, Lloreta J, Agusti AG, Gea J, Howington JA, Reed MF, Starnes SL, Harris NL, Vitucci M, Eppert BL, Motz GT, Fogel K, McGraw DW, Tichelaar JW, Orozco-Levi M
    J. Clin. Invest., 2009;119(3):636-49.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  78. Role of neurosteroids in regulating cell death and proliferation in the late gestation fetal brain.
    Authors: Yawno T, Hirst JJ, Castillo-Melendez M, Walker DW
    Neuroscience, 2009;163(3):838-47.
    Species: Ovine
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  79. Mitogen-activated protein kinase-signaling regulates the ability of Muller glia to proliferate and protect retinal neurons against excitotoxicity.
    Authors: Fischer AJ, Scott MA, Ritchey ER, Sherwood P
    Glia, 2009;57(14):1538-52.
    Species: Chicken
    Sample Type: Whole Tissue
    Application: IHC Frozen
  80. Repeated ethanol exposure during late gestation alters the maturation and innate immune status of the ovine fetal lung.
    Authors: Sozo F, O'Day L, Maritz G, Kenna K, Stacy V, Brew N, Walker D, Bocking A, Brien J, Harding R
    Am. J. Physiol. Lung Cell Mol. Physiol., 2009;296(3):L510-8.
    Species: Ovine
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  81. A functional interaction between Irx and Meis patterns the anterior hindbrain and activates krox20 expression in rhombomere 3.
    Authors: Stedman A, Lecaudey V, Havis E, Anselme I, Wassef M, Gilardi-Hebenstreit P, Schneider-Maunoury S
    Dev. Biol., 2009;327(2):566-77.
    Species: Zebrafish
    Sample Type: Whole Tissue
    Application: IHC
  82. Cisplatin-resistant neuroblastoma cells express enhanced levels of epidermal growth factor receptor (EGFR) and are sensitive to treatment with EGFR-specific toxins.
    Authors: Michaelis M, Bliss J, Arnold SC, Hinsch N, Rothweiler F, Deubzer HE, Witt O, Langer K, Doerr HW, Wels WS, Cinatl J
    Clin. Cancer Res., 2008;14(20):6531-7.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  83. The tyrosine kinase inhibitor cediranib blocks ligand-induced vascular endothelial growth factor receptor-3 activity and lymphangiogenesis.
    Authors: Heckman CA, Holopainen T, Wirzenius M, Keskitalo S, Jeltsch M, Yla-Herttuala S, Wedge SR, Jurgensmeier JM, Alitalo K
    Cancer Res., 2008;68(12):4754-62.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  84. Cystic fibrosis transmembrane conductance regulator controls lung proteasomal degradation and nuclear factor-kappaB activity in conditions of oxidative stress.
    Authors: Boncoeur E, Roque T, Bonvin E, Saint-Criq V, Bonora M, Clement A, Tabary O, Henrion-Caude A, Jacquot J
    Am. J. Pathol., 2008;172(5):1184-94.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  85. Loss of vascular endothelial growth factor expression reduces vascularization, but not growth, of tumors lacking the Von Hippel-Lindau tumor suppressor gene.
    Authors: Blouw B, Haase VH, Song H, Bergers G, Johnson RS
    Oncogene, 2007;26(31):4531-40.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  86. Telomerase reverses epidermal hair follicle stem cell defects and loss of long-term survival associated with critically short telomeres.
    Authors: Siegl-Cachedenier I, Flores I, Klatt P, Blasco MA
    J. Cell Biol., 2007;179(2):277-90.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  87. Protein C is an autocrine growth factor for human skin keratinocytes.
    Authors: Xue M, Campbell D, Jackson CJ
    J. Biol. Chem., 2007;282(18):13610-6.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  88. Inhibition of neurosteroid synthesis increases asphyxia-induced brain injury in the late gestation fetal sheep.
    Authors: Yawno T, Yan EB, Walker DW, Hirst JJ
    Neuroscience, 2007;146(4):1726-33.
    Species: Ovine
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  89. Gam1-associated alterations of drug responsiveness through activation of apoptosis.
    Authors: Wu F, Chiocca S, Beck WT, Mo YY
    Mol. Cancer Ther., 2007;6(6):1823-30.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  90. Multiple neurotoxic stresses converge on MDMX proteolysis to cause neuronal apoptosis.
    Authors: Benosman S, Gross I, Clarke N, Jochemsen AG, Okamoto K, Loeffler JP, Gaiddon C
    Cell Death Differ., 2007;14(12):2047-57.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
  91. Tendon-muscle crosstalk controls muscle bellies morphogenesis, which is mediated by cell death and retinoic acid signaling.
    Authors: Rodriguez-Guzman M, Montero JA, Santesteban E, Ganan Y, Macias D, Hurle JM
    Dev. Biol., 2007;302(1):267-80.
    Species: Chicken
    Sample Type: Whole Tissue
    Application: IHC
  92. Cytotoxic action of phorbol esters on human pancreatic cancer cells.
    Authors: Bond JA, Gescher AJ, Verschoyle RD, Lemoine NR, Errington R, Wiltshire M, Smith PJ, Wynford-Thomas D
    Int. J. Cancer, 2007;121(7):1445-54.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  93. Cytochemical and ultrastructural study of anoikis and secondary necrosis in enterocytes detached in vivo.
    Authors: do Vale A, Costa-Ramos C, Silva DS, Macedo PM, Fernandes R, Sampaio P, Dos Santos NM, Silva MT
    Apoptosis, 2007;12(6):1069-83.
    Species: Fish
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  94. Expression and functional analysis of Bax inhibitor-1 in human breast cancer cells.
    Authors: Grzmil M, Kaulfuss S, Thelen P, Hemmerlein B, Schweyer S, Obenauer S, Kang TW, Burfeind P
    J. Pathol., 2006;208(3):340-9.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  95. Human coronavirus OC43 infection induces chronic encephalitis leading to disabilities in BALB/C mice.
    Authors: Jacomy H, Fragoso G, Almazan G, Mushynski WE, Talbot PJ
    Virology, 2006;349(2):335-46.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
  96. Canonical notch signaling functions as a commitment switch in the epidermal lineage.
    Authors: Blanpain C, Lowry WE, Pasolli HA, Fuchs E
    Genes Dev., 2006;20(21):3022-35.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  97. Involvement of the Edar signaling in the control of hair follicle involution (catagen).
    Authors: Fessing MY, Sharova TY, Sharov AA, Atoyan R, Botchkarev VA
    Am. J. Pathol., 2006;169(6):2075-84.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Fresh
  98. Invasive trophoblasts stimulate vascular smooth muscle cell apoptosis by a fas ligand-dependent mechanism.
    Authors: Harris LK, Keogh RJ, Wareing M, Baker PN, Cartwright JE, Aplin JD, Whitley GS
    Am. J. Pathol., 2006;169(5):1863-74.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Frozen
  99. Mechanisms of apoptosis after ischemia and reperfusion: role of the renin-angiotensin system.
    Authors: Kossmehl P, Kurth E, Faramarzi S, Habighorst B, Shakibaei M, Wehland M, Kreutz R, Infanger M, J Danser AH, Grosse J, Paul M, Grimm D
    Apoptosis, 2006;11(3):347-58.
    Species: Porcine
    Sample Type: Tissue Homogenates
    Application: WB
  100. Evolution of the androgen receptor pathway during progression of prostate cancer.
    Authors: Hendriksen PJ, Dits NF, Kokame K, Veldhoven A, van Weerden WM, Bangma CH, Trapman J, Jenster G
    Cancer Res., 2006;66(10):5012-20.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  101. Wnt signals mediate a fate decision between otic placode and epidermis.
    Authors: Ohyama T, Mohamed OA, Taketo MM, Dufort D, Groves AK
    Development, 2006;133(5):865-75.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  102. Helicobacter felis and Helicobacter bizzozeronii induce gastric parietal cell loss in Mongolian gerbils.
    Authors: De Bock M, Decostere A, Hellemans A, Haesebrouck F, Ducatelle R
    Microbes Infect., 2006;8(2):503-10.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  103. Apoptosis and active caspase-3 expression in human granulosa cells.
    Authors: Glamoclija V, Vilovic K, Saraga-Babic M, Baranovic A, Sapunar D
    Fertil. Steril., 2005;83(2):426-31.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  104. Higher resistance of porcine trigeminal ganglion neurons towards pseudorabies virus-induced cell death compared with other porcine cell types in vitro.
    Authors: Geenen K, Favoreel HW, Nauwynck HJ
    J. Gen. Virol., 2005;86(0):1251-60.
    Species: Porcine
    Sample Type: Whole Cells
    Application: ICC
  105. The pseudorabies virus US3 protein kinase possesses anti-apoptotic activity that protects cells from apoptosis during infection and after treatment with sorbitol or staurosporine.
    Authors: Geenen K, Favoreel HW, Olsen L, Enquist LW, Nauwynck HJ
    Virology, 2005;331(1):144-50.
    Species: Porcine
    Sample Type: Whole Cells
    Application: ICC
  106. Matrix metalloproteinase-8 deficiency promotes granulocytic allergen-induced airway inflammation.
    Authors: Gueders MM, Balbin M, Rocks N, Foidart JM, Gosset P, Louis R, Shapiro S, Lopez-Otin C, Noel A, Cataldo DD
    J. Immunol., 2005;175(4):2589-97.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  107. Effects of systemically administered NT-3 on sensory neuron loss and nestin expression following axotomy.
    Authors: Kuo LT, Simpson A, Schanzer A, Tse J, An SF, Scaravilli F, Groves MJ
    J. Comp. Neurol., 2005;482(4):320-32.
    Species: Rat
    Sample Type: Whole Cells
    Application: ICC
  108. Ro/SSA autoantibodies directly bind cardiomyocytes, disturb calcium homeostasis, and mediate congenital heart block.
    Authors: Salomonsson S, Sonesson SE, Ottosson L, Muhallab S, Olsson T, Sunnerhagen M, Kuchroo VK, Thoren P, Herlenius E, Wahren-Herlenius M
    J. Exp. Med., 2005;201(1):11-7.
    Species: Rat
    Sample Type: Whole Cells
    Application: ICC
  109. Blockade of the type I IGF receptor expression in human prostate cancer cells inhibits proliferation and invasion, up-regulates IGF binding protein-3, and suppresses MMP-2 expression.
    Authors: Grzmil M, Hemmerlein B, Thelen P, Schweyer S, Burfeind P
    J. Pathol., 2004;202(1):50-9.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  110. Lactate dehydrogenase-elevating virus induces apoptosis in cultured macrophages and in spinal cords of C58 mice coincident with onset of murine amyotrophic lateral sclerosis.
    Authors: Zitterkopf NL, McNeal DW, Eyster KM, Bradley DS, Cafruny WA
    Virus Res., 2004;106(1):35-42.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
  111. Analysis of central regulatory pathways in p53-deficient primary cultures of malignant fibrous histiocytoma exposed to ifosfamide.
    Authors: Schlott T, Taubert H, Fayyazi A, Schweyer S, Bartel F, Korabiowska M, Brinck U
    Anticancer Res., 2004;24(6):3819-29.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  112. Evidence of caspase-3 activation in hyposmotic stress-induced necrosis.
    Authors: Niquet J, Allen SG, Baldwin RA, Wasterlain CG
    Neurosci. Lett., 2004;356(3):225-7.
    Species: Rat
    Sample Type: Whole Cells
    Application: ICC
  113. Early events in the anoikis program occur in the absence of caspase activation.
    Authors: Wang P, Valentijn AJ, Gilmore AP, Streuli CH
    J. Biol. Chem., 2003;278(22):19917-25.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
Expand to show all 113 Citations
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Average Rating: 4 (Based on 1 review)

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Immunohistochemistry Human/Mouse Active Caspase-3 Antibody AF835
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 IHC Anonymous 05/17/2017
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Immunohistochemistry Human/Mouse Active Caspase-3 Antibody AF835
Immunohistochemistry: Human/Mouse Active Caspase-3 Antibody [AF835]

Summary

ApplicationImmunohistochemistry
Sample TestedBrain (hippocampus)

Other Experimental Details

Other Experimental DetailsActive Caspase-3 (red) on fresh frozen, formaldehyde-fixed 10um sections of CA3 region of hippocampus. Good signal, but difficult to image because of high background from NeuN (green).

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