Detection of Human/Mouse ATF1 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and NIH-3T3 mouse embryonic fibroblast cell line. Gels were loaded with 30 μg of whole cell lysate (WCL), 20 μg of cytoplasmic (Cyto), and 10 μg of nuclear extracts (Nuc). PVDF membrane was probed with 1 µg/mL Goat Anti-Human/Mouse ATF1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4370) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band for ATF1 was detected at approximately 48 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
ATF1 is a member of the bzip family of transcription factors. ATF1, CREB, and CREM form a subfamily of bzip proteins, which respond to cAMP activation in cells by forming homo and heterodimers. These factors respond to extracellular stimulus by regulating gene expression through binding cAMP response elements (TGACGTCA).
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