Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all ATF1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human ATF1
Met1-Val271
Accession # P18846

Specificity

Detects human and mouse ATF1 in Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human/Mouse ATF1 Antibody

Detection of Human/Mouse ATF1 antibody by Western Blot.

Detection of Human/Mouse ATF1 by Western Blot.

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and NIH-3T3 mouse embryonic fibroblast cell line. Gels were loaded with 30 µg of whole cell lysate (WCL), 20 µg of cytoplasmic (Cyto), and 10 µg of nuclear extracts (Nuc). PVDF membrane was probed with 1 µg/mL Goat Anti-Human/Mouse ATF1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4370) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band for ATF1 was detected at approximately 48 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human/Mouse ATF1 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line and NIH-3T3 mouse embryonic fibroblast cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: ATF1

ATF1 is a member of the bzip family of transcription factors. ATF1, CREB, and CREM form a subfamily of bzip proteins, which respond to cAMP activation in cells by forming homo and heterodimers. These factors respond to extracellular stimulus by regulating gene expression through binding cAMP response elements (TGACGTCA).

Long Name

Activating Transcription Factor 1

Alternate Names

TREB36

Entrez Gene IDs

466 (Human); 11908 (Mouse); 315305 (Rat)

Gene Symbol

ATF1

UniProt

P18846

Additional ATF1 Products

Product Documents for Human/Mouse ATF1 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse ATF1 Antibody

For research use only

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