Detects human and mouse BACE‑1 Ectodomain in direct ELISAs. In direct ELISAs, no cross‑reactivity with recombinant human (rh) BACE-2, recombinant mouse BACE-2, rhADAM8, rhADAM9, rhADAM10, rhADAM15, or rhTACE is observed.
Monoclonal Mouse IgG1 Clone # 137626
Protein A or G purified from hybridoma culture supernatant
Mouse myeloma cell line NS0-derived recombinant human BACE-1 Thr22-Tyr460 Accession # P56817
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
<0.10 EU per 1 μg of the antibody by the LAL method.
Measured by its ability to neutralize Recombinant Human BACE‑1 (10 µg/mL, Catalog # 931-AS) or Recombinant Mouse BACE‑1 (10 µg/mL, Catalog # 2976-AS) cleavage of the fluorogenic peptide substrate Mca-SEVNLDAEFRK(Dnp)RR-NH2 (10 µM, Catalog # ES004). The Neutralization Dose (ND50) is typically 10 µg/mL.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Neutralization of BACE‑1 Activity by Human/Mouse BACE‑1 Antibody.
The cleavage of Mca-SEVNLDAEFRK(Dnp)RR-NH2 (10 µM, Catalog # ES004) by Recombinant Human BACE‑1 (10 µg/mL, Catalog # 931-AS) or Recombinant Mouse BACE‑1 (10 µg/mL, Catalog # 2976-AS) is measured after preincubation with increasing concentrations of Mouse Anti-Human/Mouse BACE‑1 Ectodomain Monoclonal Antibody (Catalog # MAB9311). The ND50 is typically 10 µg/mL.a
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
(beta‑site APP cleaving enzyme-1) is an aspartic protease and an integral membrane protein (1, 2). It is
the major beta secretase, and together with the gamma secretase, is responsible
for generating the amyloid beta peptide (A beta ) from the amyloid precursor
protein (APP) (3, 4). Because A beta is a major component of amyloid
plaques, BACE-1 has been implicated in the onset and/or progression of
Alzheimer's disease. High levels of BACE-1 activity are sufficient to
elicit neurodegeneration and neurological decline in vivo,
indicating that inhibiting BACE-1 may block not only A beta -dependent but
also A beta -independent pathogenic mechanisms (5). In addition to APP,
BACE-1 also cleaves APP-like proteins 1 and 2, the cell adhesion protein
P-selectin glycoprotein ligand-1 and beta -galactoside alpha 2,6-sialyltransferase,
implying that BACE-1 may have additional functions involving the
ectodomain shedding of membrane proteins (6 - 8).
Vassar, R. et al. (1999) Science 286:735.
Yan, R. et al. (1999) Nature 402:533.
Cai, H. et al. (2001) Nature Neurosci. 4:233.
Roberds, S.L. et al. (2001) Human Mol. Genet. 97:1317.
Rockenstein, E. et al. (2005) J. Biol. Chem. 280:32957.
Li, Q and T.C. Sudhof (2004) J. Biol. Chem. 279:10542.
Lichtenthaler, S.F. et al. (2003) J. Biol. Chem. 278:48713.
Kitazynem, S. et al. (2005) J. Biol. Chem. 280:8589.
beta-site Ayloid Precursor Protein Cleaving Enzyme 1
R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
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