Human/Mouse BACE-1 Ectodomain Antibody

(1 citations)   
  • Species Reactivity
    Human, Mouse
  • Specificity
    Detects human and mouse BACE‑1 Ectodomain in direct ELISAs. In direct ELISAs, no cross‑reactivity with recombinant human (rh) BACE-2, recombinant mouse BACE-2, rhADAM8, rhADAM9, rhADAM10, rhADAM15, or rhTACE is observed.
  • Source
    Monoclonal Mouse IgG1 Clone # 137626
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human BACE-1
    Thr22-Tyr460
    Accession # P56817
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Immunoprecipitation
    25 µg/mL
    Conditioned cell culture medium spiked with Recombinant Human BACE‑1 (Catalog # 931‑AS) or Recombinant Mouse BACE‑1 (Catalog # 2976-AS), see our available Western blot detection antibodies
  • Neutralization
    Measured by its ability to neutralize Recombinant Human BACE‑1 (10 µg/mL, Catalog # 931-AS) or Recombinant Mouse BACE‑1 (10 µg/mL, Catalog # 2976-AS) cleavage of the fluorogenic peptide substrate Mca-SEVNLDAEFRK(Dnp)RR-NH2 (10 µM, Catalog # ES004). The Neutralization Dose (ND50) is typically 10 µg/mL.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Neutralization of BACE‑1 Activity by Human/Mouse BACE‑1 Antibody.

The cleavage of Mca-SEVNLDAEFRK(Dnp)RR-NH2 (10 µM, Catalog # ES004) by Recombinant Human BACE‑1 (10 µg/mL, Catalog # 931-AS) or Recombinant Mouse BACE‑1 (10 µg/mL, Catalog # 2976-AS) is measured after preincubation with increasing concentrations of Mouse Anti-Human/Mouse BACE‑1 Ectodomain Monoclonal Antibody (Catalog # MAB9311). The ND50 is typically 10 µg/mL.a

Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: BACE-1
BACE-1 (beta‑site APP cleaving enzyme-1) is an aspartic protease and an integral membrane protein (1, 2). It is the major beta secretase, and together with the gamma secretase, is responsible for generating the amyloid beta peptide (A beta ) from the amyloid precursor protein (APP) (3, 4). Because A beta is a major component of amyloid plaques, BACE-1 has been implicated in the onset and/or progression of Alzheimer's disease. High levels of BACE-1 activity are sufficient to elicit neurodegeneration and neurological decline in vivo, indicating that inhibiting BACE-1 may block not only A beta -dependent but also A beta -independent pathogenic mechanisms (5). In addition to APP, BACE-1 also cleaves APP-like proteins 1 and 2, the cell adhesion protein P-selectin glycoprotein ligand-1 and beta -galactoside alpha 2,6-sialyltransferase, implying that BACE-1 may have additional functions involving the ectodomain shedding of membrane proteins (6 - 8).
  • References:
    1. Vassar, R. et al. (1999) Science 286:735.
    2. Yan, R. et al. (1999) Nature 402:533.
    3. Cai, H. et al. (2001) Nature Neurosci. 4:233.
    4. Roberds, S.L. et al. (2001) Human Mol. Genet. 97:1317.
    5. Rockenstein, E. et al. (2005) J. Biol. Chem. 280:32957.
    6. Li, Q and T.C. Sudhof (2004) J. Biol. Chem. 279:10542.
    7. Lichtenthaler, S.F. et al. (2003) J. Biol. Chem. 278:48713.
    8. Kitazynem, S. et al. (2005) J. Biol. Chem. 280:8589.
  • Long Name:
    beta-site Ayloid Precursor Protein Cleaving Enzyme 1
  • Entrez Gene IDs:
    23621 (Human); 23821 (Mouse)
  • Alternate Names:
    APP beta-secretase; ASP2; Aspartyl protease 2; BACE1; BACE-1; BACEAsp 2; beta-secretase 1 precursor variant 1; beta-secretase 1; beta-site amyloid beta A4 precursor protein-cleaving enzyme; Beta-site amyloid precursor protein cleaving enzyme 1; Beta-site APP cleaving enzyme 1; beta-site APP-cleaving enzyme 1; beta-site APP-cleaving enzyme; EC 3.4.23; EC 3.4.23.46; FLJ90568; HSPC104; KIAA1149; memapsin-2; Membrane-associated aspartic protease 2; transmembrane aspartic proteinase Asp2
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. A reversible form of lysine acetylation in the ER and Golgi lumen controls the molecular stabilization of BACE1.
    Authors: Costantini C, Ko MH, Jonas MC, Puglielli L
    Biochem. J., 2007;407(3):383-95.
    Species: Human
    Sample Type: Cell Lysates
    Application: IP
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Mouse IgG1 Isotype Control

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Mouse IgG HRP-conjugated Antibody

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Donkey Anti-Mouse IgG NorthernLights™ NL637-conjugated Antibody

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Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody

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Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

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Mouse IgG Antibody

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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

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Mouse IgG VisUCyte HRP Polymer Antibody

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Goat Anti-Mouse IgG Fc Affinity Purified Polyclonal Ab

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Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0114  
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