Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Western Blot, Immunoprecipitation

Cited:

Western Blot, ELISA Capture, ELISA Detection

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all BACE-1 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human BACE‑1 Ectodomain
Thr22-Tyr460
Accession # P56817

Specificity

Detects human and mouse BACE‑1 Ectodomain in direct ELISAs and Western blots. In direct ELISAs, less than 2% cross‑reactivity with recombinant human (rh) ADAM8, recombinant mouse ADAM9, rhADAM15, and rhTACE is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Human/Mouse BACE‑1 Ectodomain Antibody

Application
Recommended Usage

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human BACE‑1 (Catalog # 931‑AS) or Recombinant Mouse BACE‑1 (Catalog # 2976-AS), see our available Western blot detection antibodies

Western Blot

0.1 µg/mL
Sample: Recombinant Human BACE‑1 (Catalog # 931‑AS)
Recombinant Mouse BACE‑1 (Catalog # 2976‑AS)

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: BACE-1

BACE-1 is an aspartic protease and an integral membrane protein (1 - 5). BACE-1 is the peptidase predominantly responsible for cleavage of the amyloid precursor protein beta site in the brain to generate the amyloid beta peptide. Because the amyloid beta peptide is a major component of amyloid plaques, BACE-1 has been implicated in the onset and/or progression of Alzheimer's disease. BACE-1 is expressed in a variety of human tissues. It is likely that this peptidase has functions in addition to its hydrolysis of the amyloid precursor protein. The peptidase activity of BACE-1 is optimal under mildly acidic conditions (pH 3.5 - 5.5), consistent with its proposed function in an acidic intracellular compartment.

References

  1. Ermolieff, J. et al. (2000) Biochemistry 39:12450.
  2. Lin, X. et al. (2000) Proc. Natl. Acad. Sci. USA 97:1456.
  3. Sinha, S. et al. (1999) Nature 402:537.
  4. Vassar, R. et al. (1999) Science 286:735.
  5. Yan, R. et al. (1999) Nature 402:533.

Long Name

beta-site Ayloid Precursor Protein Cleaving Enzyme 1

Alternate Names

BACE1

Entrez Gene IDs

23621 (Human); 23821 (Mouse)

Gene Symbol

BACE1

UniProt

P56817

Additional BACE-1 Products

Product Documents for Human/Mouse BACE‑1 Ectodomain Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human/Mouse BACE‑1 Ectodomain Antibody

For research use only

Related Research Areas

Aspartic Proteases and Regulators

Citations for Human/Mouse BACE‑1 Ectodomain Antibody

Customer Reviews for Human/Mouse BACE‑1 Ectodomain Antibody

There are currently no reviews for this product. Be the first to review Human/Mouse BACE‑1 Ectodomain Antibody and earn rewards!

Have you used Human/Mouse BACE‑1 Ectodomain Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies