Human/Mouse Bcl-2 Antibody

Catalog # Availability Size / Price Qty
AF810
AF810-SP
Detection of Human/Mouse Bcl‑2 by Western Blot.
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Product Details
Citations (4)
FAQs
Supplemental Products
Reviews (1)

Human/Mouse Bcl-2 Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human and mouse Bcl-2 in Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse Bcl-2
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Simple Western
50 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
See below
Immunoprecipitation
1 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human/Mouse Bcl‑2 by Western Blot. View Larger

Detection of Human/Mouse Bcl‑2 by Western Blot. Western blot shows lysates of KG‑1 human myeloid leukemia cell line and M‑NFS‑60 mouse myelogenous leukemia lymphoblast cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse Bcl‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF810) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Bcl‑2, at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Immunohistochemistry Bcl-2 in Human Breast Cancer Tissue. View Larger

Bcl-2 in Human Breast Cancer Tissue. Bcl‑2 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using 15 µg/mL Goat Anti-Human/
Mouse Bcl‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF810) overnight at 4 °C. Tissue was stained (red) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Immunoprecipitation Immunoprecipitation of Mouse Bcl‑2. View Larger

Immunoprecipitation of Mouse Bcl‑2. Bcl‑2 was immunoprecipitated from lysates (3 x 106 cells) of M‑NFS‑60 mouse myelogenous leukemia lymphoblast cell line following incubation with 3 µg Goat Anti-Human/Mouse Bcl‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF810) for 1 hour at 4 °C. Bcl‑2-antibody complexes were absorbed using Protein G (Sigma). Immunoprecipitated Bcl‑2 was detected by Western blot using 1 µg/mL Goat Anti-Human/Mouse Bcl‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF810). View our recommended buffer recipes for immunoprecipitation.

Simple Western Detection of Human Bcl‑2 by Simple WesternTM. View Larger

Detection of Human Bcl‑2 by Simple WesternTM. Simple Western lane view shows lysates of KG‑1 human acute myelogenous leukemia cell line, loaded at 0.2 mg/mL. A specific band was detected for Bcl‑2 at approximately 33 kDa (as indicated) using 50 µg/mL of Goat Anti-Human/Mouse Bcl‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF810) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the
12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Bcl-2

Bcl-2 is a member of a family of proteins that regulates outer mitochondrial membrane permeability (1, 2). Bcl-2 is an anti-apoptotic member that prevents release of cytochrome c from the mitochondria intermembrane space into the cytosol. Bcl-2 is present on the outer mitochondrial membrane and is also found on other membranes in some cell types. Natural Bcl-2 contains a carboxyl-terminal mitochondria targeting sequence. Recombinant Bcl-2, missing the mitochondrial targeting sequence, maintains its ability to neutralize pro-apoptotic Bcl-2 family members. Neutralization by Bcl-2 appears to be through binding the BH3 region of pro-apoptotic Bcl-2 family members. This activity does not require the mitochondrial targeting sequence.

 

References
  1. Gross, A. et al. (1999) Genes and Develop. 13:1899.
  2. Kroemer, G. (1997) Nature Med. 3:614.
Long Name
B Cell Lymphoma/Leukemia 2
Entrez Gene IDs
596 (Human); 12043 (Mouse); 24224 (Rat)
Alternate Names
apoptosis regulator Bcl-2; B-cell CLL/lymphoma 2; Bcl2; Bcl-2

Product Datasheets

Citations for Human/Mouse Bcl-2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. B-cell lymphoma 2 is associated with advanced tumor grade and clinical stage, and reduced overall survival in young Chinese patients with colorectal carcinoma
    Authors: J Wang, G He, Q Yang, L Bai, B Jian, Q Li, Z Li
    Oncol Lett, 2018;15(6):9009-9016.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  2. TCDD promotes lung tumors via attenuation of apoptosis through activation of the Akt and ERK1/2 signaling pathways.
    Authors: Chen R, Siao S, Hsu C, Chang C, Chang L, Wu C, Lin P, Wang Y
    PLoS ONE, 2014;9(6):e99586.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Bioassay
  3. Molecular profiling of cervical cancer progression.
    Authors: Hagemann T, Bozanovic T, Hooper S, Ljubic A, Slettenaar VI, Wilson JL, Singh N, Gayther SA, Shepherd JH, Van Trappen PO
    Br. J. Cancer, 2007;96(2):321-8.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC Paraffin-embedded
  4. The anti-apoptotic effect of Notch-1 requires p56lck-dependent, Akt/PKB-mediated signaling in T cells.
    Authors: Sade H, Krishna S, Sarin A
    J. Biol. Chem., 2004;279(4):2937-44.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

FAQs

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Cell and Tissue Staining Kits

ExactaChIP Chromatin Immunoprecipitation Kits

Immunohistochemistry Reagents

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Human/Mouse Bcl-2 Antibody

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Human/Mouse Bcl-2 Antibody
By Anonymous on 01/11/2018
Application: WB Sample Tested: MDA-MBA-231 human breast cancer cell line Species: Human