Human/Mouse Bcl-2 Antibody

Catalog # Availability Size / Price Qty
AF810
AF810-SP
Detection of Human/Mouse Bcl‑2 by Western Blot.
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Product Details
Citations (13)
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Supplemental Products
Reviews (1)

Human/Mouse Bcl-2 Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human and mouse Bcl-2 in Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse Bcl-2
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Simple Western
50 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
See below
Immunoprecipitation
1 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human/Mouse Bcl-2 antibody by Western Blot. View Larger

Detection of Human/Mouse Bcl‑2 by Western Blot. Western blot shows lysates of KG-1 human myeloid leukemia cell line and M-NFS-60 mouse myelogenous leukemia lymphoblast cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse Bcl-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF810) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Bcl-2, at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Immunohistochemistry Bcl-2 antibody in Human Breast Cancer Tissue by Immunohistochemistry (IHC-P). View Larger

Bcl-2 in Human Breast Cancer Tissue. Bcl‑2 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using 15 µg/mL Goat Anti-Human/ Mouse Bcl‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF810) overnight at 4 °C. Tissue was stained (red) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Immunoprecipitation Immunoprecipitation of Mouse Bcl‑2. View Larger

Immunoprecipitation of Mouse Bcl‑2. Bcl‑2 was immunoprecipitated from lysates (3 x 106cells) of M‑NFS‑60 mouse myelogenous leukemia lymphoblast cell line following incubation with 3 µg Goat Anti-Human/Mouse Bcl‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF810) for 1 hour at 4 °C. Bcl‑2-antibody complexes were absorbed using Protein G (Sigma). Immunoprecipitated Bcl‑2 was detected by Western blot using 1 µg/mL Goat Anti-Human/Mouse Bcl‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF810). View our recommended buffer recipes for immunoprecipitation.

Simple Western Detection of Human Bcl-2 antibody by Simple Western<SUP>TM</SUP>. View Larger

Detection of Human Bcl‑2 by Simple WesternTM. Simple Western lane view shows lysates of KG-1 human acute myelogenous leukemia cell line, loaded at 0.2 mg/mL. A specific band was detected for Bcl-2 at approximately 33 kDa (as indicated) using 50 µg/mL of Goat Anti-Human/Mouse Bcl-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF810) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Bcl-2

Bcl-2 is a member of a family of proteins that regulates outer mitochondrial membrane permeability (1, 2). Bcl-2 is an anti-apoptotic member that prevents release of cytochrome c from the mitochondria intermembrane space into the cytosol. Bcl-2 is present on the outer mitochondrial membrane and is also found on other membranes in some cell types. Natural Bcl-2 contains a carboxyl-terminal mitochondria targeting sequence. Recombinant Bcl-2, missing the mitochondrial targeting sequence, maintains its ability to neutralize pro-apoptotic Bcl-2 family members. Neutralization by Bcl-2 appears to be through binding the BH3 region of pro-apoptotic Bcl-2 family members. This activity does not require the mitochondrial targeting sequence.

 

References
  1. Gross, A. et al. (1999) Genes and Develop. 13:1899.
  2. Kroemer, G. (1997) Nature Med. 3:614.
Long Name
B Cell Lymphoma/Leukemia 2
Entrez Gene IDs
596 (Human); 12043 (Mouse); 24224 (Rat)
Alternate Names
apoptosis regulator Bcl-2; B-cell CLL/lymphoma 2; Bcl2; Bcl-2

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Citations for Human/Mouse Bcl-2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

13 Citations: Showing 1 - 10
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  1. Interleukin‑22 regulates gastric cancer cell proliferation through regulation of the JNK signaling pathway
    Authors: Hao Dong, Fengming Zhu, Shilu Jin, Jing Tian
    Experimental and Therapeutic Medicine
  2. Targeting Cell Death Mechanism Specifically in Triple Negative Breast Cancer Cell Lines
    Authors: Lavinia-Lorena Pruteanu, Cornelia Braicu, Dezső Módos, Maria-Ancuţa Jurj, Lajos-Zsolt Raduly, Oana Zănoagă et al.
    International Journal of Molecular Sciences
  3. Knockdown of lncRNA CCAT1 Inhibits the Progression of Colorectal Cancer via hsa-miR-4679 Mediating the Downregulation of GNG10
    Authors: N Wang, J Li, J He, YG Jing, WD Zhao, WJ Yu, J Wang
    Journal of Immunology Research, 2021-12-30;2021(0):8930813.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  4. Lipid storage droplet protein 5 reduces sodium palmitate?induced lipotoxicity in human normal liver cells by regulating lipid metabolism?related factors
    Authors: X Ma, F Cheng, K Yuan, K Jiang, T Zhu
    Mol Med Rep, 2019-06-06;20(2):879-886.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  5. B-cell lymphoma 2 is associated with advanced tumor grade and clinical stage, and reduced overall survival in young Chinese patients with colorectal carcinoma
    Authors: J Wang, G He, Q Yang, L Bai, B Jian, Q Li, Z Li
    Oncol Lett, 2018-04-13;15(6):9009-9016.
    Species: Human
    Sample Types: Tissue Homogenates, Whole Tissue
    Applications: IHC-P, Western Blot
  6. TCDD promotes lung tumors via attenuation of apoptosis through activation of the Akt and ERK1/2 signaling pathways.
    Authors: Chen R, Siao S, Hsu C, Chang C, Chang L, Wu C, Lin P, Wang Y
    PLoS ONE, 2014-06-13;9(6):e99586.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Bioassay
  7. Molecular profiling of cervical cancer progression.
    Authors: Hagemann T, Bozanovic T, Hooper S, Ljubic A, Slettenaar VI, Wilson JL, Singh N, Gayther SA, Shepherd JH, Van Trappen PO
    Br. J. Cancer, 2007-01-29;96(2):321-8.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  8. The anti-apoptotic effect of Notch-1 requires p56lck-dependent, Akt/PKB-mediated signaling in T cells.
    Authors: Sade H, Krishna S, Sarin A
    J. Biol. Chem., 2003-10-28;279(4):2937-44.
    Species: Human, Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  9. TGF‑ beta 1 mediates the effects of aspirin on colonic tumor cell proliferation and apoptosis
    Authors: Yuyi Wang, Chi Du, Nan Zhang, Mei Li, Yanyang Liu, Maoyuan Zhao et al.
    Oncology Letters
  10. Securidaca–saponins are natural inhibitors of AKT, MCL-1, and BCL2L1 in cervical cancer cells
    Authors: Titus Chukwuemeka Obasi, Cornelia Braicu, Bogdan Cezar Iacob, Ede Bodoki, Ancuta Jurj, Lajos Raduly et al.
    Cancer Management and Research
  11. Genipin protects against H2O2-induced oxidative damage in�retinal pigment epithelial cells by promoting Nrf2 signaling
    Authors: Hailan Zhao, Ruiqing Wang, Mingxia Ye, Lan Zhang
    International Journal of Molecular Medicine
  12. Influence mechanism of miRNA‑144 on proliferation and apoptosis of osteosarcoma cells
    Authors: Xu Zhang, Zhengwei Li, Wei Ji, Xilong Chen, Qiang Gao, Dajin Li et al.
    Oncology Letters
  13. Protein Synthesis Inhibition Activity of Mesothelin Targeting Immunotoxin LMB-100 Decreases Concentrations of Oncogenic Signaling Molecules and Secreted Growth Factors
    Authors: Salma El-Behaedi, Rebekah Landsman, Michael Rudloff, Emily Kolyvas, Rakan Albalawy, Xianyu Zhang et al.
    Toxins (Basel)

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Human/Mouse Bcl-2 Antibody
By Jothi Jayavel on 01/11/2018
Application: WB Sample Tested: MDA-MBA-231 human breast cancer cell line Species: Human