Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot, Simple Western

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Bcl-6 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse Bcl-6
Glu522-Thr696
Accession # Q8CB25

Specificity

Detects human and mouse Bcl-6 in Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human/Mouse Bcl‑6 Antibody

Detection of Mouse and Human Bcl-6 antibody by Western Blot.

Detection of Mouse and Human Bcl‑6 by Western Blot.

Western blot shows lysates of NIH-3T3 mouse embryonic fibroblast cell line, HeLa human cervical epithelial carcinoma cell line, and 293T human embryonic kidney cell line. PVDF membrane was probed with 0.1 µg/mL of Mouse Bcl-6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5046) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Bcl-6 at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Human Bcl‑6 by Simple WesternTM.

Simple Western lane view shows lysates of Ramos human Burkitt's lymphoma cell line and Raji human Burkitt's lymphoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Bcl‑6 at approximately 111 kDa (as indicated) using 10 µg/mL of Goat Anti-Human/Mouse Bcl‑6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5046). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications for Human/Mouse Bcl‑6 Antibody

Application
Recommended Usage

Simple Western

10 µg/mL
Sample: Ramos human Burkitt's lymphoma cell line and Raji human Burkitt's lymphoma cell line

Western Blot

0.1 µg/mL
Sample: NIH‑3T3 mouse embryonic fibroblast cell line, HeLa human cervical epithelial carcinoma cell line, and 293T human embryonic kidney cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Bcl-6

Bcl-6 (B-cell lymphoma protein 6; also ZFP51, LAZ-3 and Bcl-5) is an 80 kDa member of the Kruppel-like family of zinc finger proteins. It is expressed in germinal center B cells, neutrophils, monocytes, and immature dendritic cells, and serves as a potent transcriptional repressor of genes such as p53. Human Bcl-6 is 706 amino acids (aa) in length and contains an N-terminal BTB/POZ protein interaction domain (aa 32‑99) and a series of six C2H2-type C-terminal zinc finger domains that bind DNA (aa 518‑681). Centrally located are three PEST sequences (aa 336‑430) that undergo phosphorylation by MAPK. This increases the molecular weight of Bcl-6 to 97 kDa and induces its degradation. Human Bcl-6 is about 99% aa identical to mouse and canine Bcl-6.

Long Name

B Cell Lymphoma/Leukemia 6

Alternate Names

Bcl-5, Bcl6, LAZ3, ZBTB27, ZNF51

Entrez Gene IDs

604 (Human); 12053 (Mouse); 303836 (Rat)

Gene Symbol

BCL6

UniProt

Q8CB25

Additional Bcl-6 Products

Product Documents for Human/Mouse Bcl‑6 Antibody

Certificate of Analysis

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Product Specific Notices for Human/Mouse Bcl‑6 Antibody

For research use only

Citations for Human/Mouse Bcl‑6 Antibody

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