Detection of Human and Mouse Cadherin‑17 by Western Blot.
Western blot shows lysates of human colon tissue and mouse colon tissue. PVDF membrane was probed with 1:1000 dilution of Rabbit Anti-Human/Mouse Cadherin‑17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8524) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Cadherin‑17 at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Cadherin‑17 in Mouse Intestine.
Cadherin‑17 was detected in perfusion fixed frozen sections of mouse intestine using Rabbit Anti-Mouse Cadherin‑17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8524) at 1:250 dilution overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to the plasma membrane of glandular cells. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
Detection of Human and mouse Cadherin‑17 by Simple WesternTM.
Simple Western lane view shows lysates of human colon tissue and mouse colon tissue, loaded at 0.2 mg/mL. Specific bands were detected for Cadherin‑17 at approximately 152 and 146 kDa (as indicated) using 1:100 dilution of Rabbit Anti-Human/Mouse Cadherin‑17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8524). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C, as supplied.
1 month, 2 to 8 °C under sterile conditions after opening.
6 months, -20 to -70 °C under sterile conditions after opening.
Cadherin-17, also known as liver-intestine (LI) Cadherin, belongs to the cadherin family of calcium-dependent cell adhesion molecules. It associates homotypically and is selectively expressed on gastric and intestinal mucosa.
Entrez Gene IDs:
1015 (Human); 12557 (Mouse)
cadherin 17, LI cadherin (liver-intestine); cadherin; cadherin-16; Cadherin17; Cadherin-17; CDH16; CDH17; FLJ26931; HPT-1 cadherin; HPT1; HPT-1; human intestinal peptide-associated transporter HPT-1; human peptide transporter 1; Intestinal peptide-associated transporter HPT-1; LI cadherin; LI-cadherin; Liver-intestine cadherin; MGC138218; MGC142024
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