Key Product Details
Species Reactivity
Applications
Label
Antibody Source
Product Specifications
Immunogen
Glu22-Met786
Accession # Q9R100
Specificity
Clonality
Host
Isotype
Scientific Data Images for Human/Mouse Cadherin‑17 Antibody
Detection of Human and Mouse Cadherin‑17 by Western Blot.
Western blot shows lysates of human colon tissue and mouse colon tissue. PVDF membrane was probed with 1:1000 dilution of Rabbit Anti-Human/Mouse Cadherin-17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8524) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Cadherin-17 at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Cadherin‑17 in Mouse Intestine.
Cadherin-17 was detected in perfusion fixed frozen sections of mouse intestine using Rabbit Anti-Mouse Cadherin-17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8524) at 1:250 dilution overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to the plasma membrane of glandular cells. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
Detection of Human and mouse Cadherin‑17 by Simple WesternTM.
Simple Western lane view shows lysates of human colon tissue and mouse colon tissue, loaded at 0.2 mg/mL. Specific bands were detected for Cadherin‑17 at approximately 152 and 146 kDa (as indicated) using 1:100 dilution of Rabbit Anti-Human/Mouse Cadherin‑17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8524). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Applications for Human/Mouse Cadherin‑17 Antibody
Immunohistochemistry
Sample: Perfusion fixed frozen sections of mouse intestine
Simple Western
Sample: Human colon tissue and mouse colon tissue
Western Blot
Sample: Human colon tissue and mouse colon tissue
Formulation, Preparation, and Storage
Purification
Formulation
Shipping
Stability & Storage
- 12 months from date of receipt, -20 to -70 °C, as supplied.
- 1 month, 2 to 8 °C under sterile conditions after opening.
- 6 months, -20 to -70 °C under sterile conditions after opening.
Background: Cadherin-17
Alternate Names
Gene Symbol
UniProt
Additional Cadherin-17 Products
Product Documents for Human/Mouse Cadherin‑17 Antibody
Product Specific Notices for Human/Mouse Cadherin‑17 Antibody
* Contains 0.1% Sodium Azide, which is not hazardous at this concentration according to GHS classifications. Refer to SDS for additional information and handling instructions.
For research use only
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars