Detects human Carbonic Anhydrase VIII/CA8 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant human (rh) CA2 and rhCA3 is observed and less than 1% cross-reactivity with rhCA1, rhCA4, and rhCA9 is observed.
Polyclonal Goat IgG
E. coli-derived recombinant human Carbonic Anhydrase VIII/CA8 Ala2-Gln290 Accession # P35219
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Detection of Human and Mouse Carbonic Anhydrase VIII/CA8 by Western Blot.
Western blot shows lysates of human brain (cerebellum) tissue and mouse brain (cerebellum) tissue. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse Carbonic Anhydrase VIII/CA8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2187) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Carbonic Anhydrase VIII/CA8 at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human and Mouse Carbonic Anhydrase VIII/CA8 by Simple WesternTM.
Simple Western lane view shows lysates of human brain (cerebellum) tissue and mouse brain (cerebellum) tissue, loaded at 0.2 mg/mL. Specific bands were detected for Carbonic Anhydrase VIII/CA8 at approximately 41 and 50 kDa (as indicated) using 5 µg/mL of Goat Anti-Human/Mouse Carbonic Anhydrase VIII/CA8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2187) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Carbonic Anhydrase VIII/CA8
Carbonic Anhydrase (CA) catalyzes the reversible reaction of CO2 + H2O = HCO3- + H+, which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption (1). Topics in a CA meeting (6th International Conference on the CAs, June 20‑25, 2003, Slovakia) ranged from the use of CAs as markers for tumor and hypoxia in the clinic, as a nutritional supplement in milk, and as a tool for CO2 removal and mosquito control in industry. CA8, also called CA‑related protein (CARP), is a cytosolic protein without CA activity (i.e., the reversible hydration of CO2) due to point mutations in the zinc-binding site (2). Nevertheless, it has esterase activity described in the Activity Assay Protocol. CA8 is expressed exclusively in Purkinje cells of the cerebellum, where it binds inositol 1,4,5‑triphosphate receptor type 1 (3). CA8 overexpression in human colorectal cancer and non-small cell lung cancer indicates that it plays a role in the process of invasion in these types of malignancy (4, 5).
Hewett-Emmett, D. and R.E. Tashian (1996) Mol. Phylogenet. Evol. 5:50.
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