|Detection of Human and Mouse Caspase‑7 Precursor and Small Subunit by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line untreated (-) or treated (+) with 1 μM staurosporine (STS) for for the indicated times and NIH‑3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Rabbit Anti- Human/Mouse Caspase‑7 Polyclonal Antibody (Catalog # AF823), followed by HRP‑conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Specific bands were detected for Caspase‑7 precursor and small subunit at approximately 32 and 7 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.|
Caspase-7 (Cysteine-aspartic acid protease 7/Casp7; also CMH-1, ICE-LAP3 and Mch3) is a 32 kDa member of the peptidase C14A/IL-1 beta -converting family of enzymes (1, 2, 3). It is widely expressed, except in brain, and is best known as an integral component of the apoptotic cascade. Caspase-7 is considered to be an executioner caspase, as a downstream mediator of apoptotic-associated proteolysis (2, 3). Upon activation, Caspase-7 is known to utilize a Cys residue to cleave multiple substrates, including PARP, procaspase 6, Gas2 and calpstatin (1). Human procaspase-7 is a 34‑36 kDa, 303 amino acid (aa) protein (4, 5, 6). Normally, it is an inactive homodimer (1, 2, 7, 8). But following an upstream signal that activates processing proteases, procaspase-7 undergoes proteolytic cleavage to generate an N-terminal 23 aa propeptide, a 175 aa p20/20 kDa subunit (aa 24‑198), and a 105 aa C-terminal p12/12 kDa subunit (5). The p20 and p12 subunits noncovalently heterodimerize, and subsequently associate with another p20/p12 heterodimer to form an active antiparallel homodimer. Additional processing of p20 may remove aa 24‑36 to generate p18, while additional processing of p12 will remove aa 199‑206 to generate p11 (9, 10). Multiple proteases can use Caspase-7 as a substrate, and include caspase-1, -3, -8, and -10, granzyme B, calpain-1 and Caspase-7 itself (3, 6, 9, 11). Caspase-7 is found in both cytosol and nucleus, and possesses a potential KKKK nuclear localization signal between aa 38‑41 that likely undergoes sumoylation (9, 12). There are two potential isoform variants, one which shows an alternate start site 33 aa upstream of the standard start site, and a second that shows a 105 aa substitution for aa 149‑303. Human and mouse Caspase-7 are 82% aa identical at the amino acid level.
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