Human/Mouse Caspase-7 Antibody

(1 citations)   
  • Species Reactivity
    Human, Mouse
  • Specificity
    Detects human and mouse Caspase-7 precursor and the small Caspase-7 subunit that is generated during proteolytic activation.
  • Source
    Polyclonal Rabbit IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    KLH-coupled mouse Caspase-7 synthetic peptide
    RHFESQSDDPRFNEKC
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human and Mouse Caspase‑7 Precursor and Small Subunit by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line untreated (-) or treated (+) with 1 μM staurosporine (STS) for for the indicated times and NIH‑3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Rabbit Anti- Human/Mouse Caspase‑7 Polyclonal Antibody (Catalog # AF823), followed by HRP‑conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Specific bands were detected for Caspase‑7 precursor and small subunit at approximately 32 and 7 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Caspase-7

Caspase-7 (Cysteine-aspartic acid protease 7/Casp7; also CMH-1, ICE-LAP3 and Mch3) is a 32 kDa member of the peptidase C14A/IL-1 beta -converting family of enzymes (1, 2, 3). It is widely expressed, except in brain, and is best known as an integral component of the apoptotic cascade. Caspase-7 is considered to be an executioner caspase, as a downstream mediator of apoptotic-associated proteolysis (2, 3). Upon activation, Caspase-7 is known to utilize a Cys residue to cleave multiple substrates, including PARP, procaspase 6, Gas2 and calpstatin (1). Human procaspase-7 is a 34‑36 kDa, 303 amino acid (aa) protein (4, 5, 6). Normally, it is an inactive homodimer (1, 2, 7, 8). But following an upstream signal that activates processing proteases, procaspase-7 undergoes proteolytic cleavage to generate an N-terminal 23 aa propeptide, a 175 aa p20/20 kDa subunit (aa 24‑198), and a 105 aa C-terminal p12/12 kDa subunit (5). The p20 and p12 subunits noncovalently heterodimerize, and subsequently associate with another p20/p12 heterodimer to form an active antiparallel homodimer. Additional processing of p20 may remove aa 24‑36 to generate p18, while additional processing of p12 will remove aa 199‑206 to generate p11 (9, 10). Multiple proteases can use Caspase-7 as a substrate, and include caspase-1, -3, -8, and -10, granzyme B, calpain-1 and Caspase-7 itself (3, 6, 9, 11). Caspase-7 is found in both cytosol and nucleus, and possesses a potential KKKK nuclear localization signal between aa 38‑41 that likely undergoes sumoylation (9, 12). There are two potential isoform variants, one which shows an alternate start site 33 aa upstream of the standard start site, and a second that shows a 105 aa substitution for aa 149‑303. Human and mouse Caspase-7 are 82% aa identical at the amino acid level.

  • References:
    1. Chowdhury, I. et al. (2008) Comp. Biochem. Physiol. B 151:10.
    2. Boatright, K.M. and G.S. Salvesen (2003) Curr. Opin. Cell Biol. 15:725.
    3. Launay, S. et al. (2005) Oncogene 24:5137.
    4. Juan, T. et al. (1997) Genomics 40:86.
    5. Fernandez-Alnemri, T. et al. (1995) Cancer Res. 55:6045.
    6. Fernandez-Alnemri, T. et al. (1996) Proc. Natl. Acad. Sci. USA 93:7464.
    7. Gao, Z. et al. (2007) J. Biol. Chem. 282:30718.
    8. Riedl, S.J. et al. (2001) Proc. Natl. Acad. Sci. USA 98:14790.
    9. Gafni, J. et al. (2009) J. Biol. Chem. July 21 [epub ahead of print].
    10. Lippke, J.A. et al. (1996) J. Biol. Chem. 271:1825.
    11. Lamkanfi, M. et al. (2008) Mol. Cell. Proteomics 7:2350.
    12. Hayashi, N. et al. (2006) Neurosci. Lett. 397:5.
  • Entrez Gene IDs:
    840 (Human); 12369 (Mouse)
  • Alternate Names:
    apoptosis-related cysteine protease; Apoptotic protease Mch-3; CASP7; CASP-7; caspase 7, apoptosis-related cysteine peptidase; Caspase7; Caspase-7; EC 3.4.22; EC 3.4.22.60; ICE-like apoptotic protease 3; Lice2 alpha/beta/gamma; Mch3
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. The B30.2 domain of pyrin, the familial Mediterranean fever protein, interacts directly with caspase-1 to modulate IL-1beta production.
    Authors: Chae JJ, Wood G, Masters SL, Richard K, Park G, Smith BJ, Kastner DL
    Proc. Natl. Acad. Sci. U.S.A., 2006;103(26):9982-7.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
Isotype Controls
Description Application Cat# Citations Images  

Normal Rabbit IgG Control

Ctrl AB-105-C 43  
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Normal Rabbit IgG Control

Ctrl, CyTOF-ready MAB1050  
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Secondary Antibodies
Description Application Cat# Citations Images  

Rabbit IgG HRP-conjugated Antibody

WB HAF008 23  
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Donkey Anti-Rabbit IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL004 10
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Donkey Anti-Rabbit IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL005 2
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Rabbit IgG APC-conjugated Antibody

Flow F0111 1  
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Rabbit IgG PE-conjugated Antibody

Flow F0110 2  
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Rabbit IgG Fluorescein-conjugated Antibody

Flow F0112 5  
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Donkey Anti-Rabbit IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL006 4
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Goat Anti-Rabbit IgG Biotinylated Antibody

WB BAF008 4
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
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Rabbit IgG Antibody

WB AF008 1
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Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC003  
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Donkey Anti-Rabbit IgG (H+L) Antibody

D-301-C-ABS2
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