Human/Mouse Cathepsin L Antibody

Catalog # Availability Size / Price Qty
MAB9521-SP
MAB9521-500
MAB9521-100
Detection of Recombinant Human and Mouse Cathepsin L by Western Blot.
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Product Details
Citations (10)
FAQs
Supplemental Products
Reviews (4)

Human/Mouse Cathepsin L Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human and mouse Cathepsin L in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human Cathepsin B, C, L2, O, S, or X/Z/P is observed. In Western blots, both the pro and active forms of recombinant human and mouse Cathspsin L are recognized.
Source
Monoclonal Rat IgG2A Clone # 204101
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Cathepsin L
Glu113-Val333
Accession # P07711
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Immunohistochemistry
5-25 µg/mL
See below
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Human Cathepsin L (Catalog # 952-CY) or Recombinant Mouse Cathepsin L (Catalog # 1515-CY), see our available Western blot detection antibodies. This antibody did not immunoprecipitate the pro form of recombinant mouse Cathepsin L.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Recombinant Human and Mouse Cathepsin L antibody by Western Blot. View Larger

Detection of Recombinant Human and Mouse Cathepsin L by Western Blot. Western blot shows 100 ng of Recombinant Human Cathepsin L (Catalog # 952-CY), Recombinant Mouse Cathepsin L (Catalog # 1515-CY), Recombinant Human Cathepsin V (Catalog # 1080-CY), Recombinant Human Cathepsin K, Recombinant Human Cathepsin S (Catalog # 1183-CY), Recombinant Human Cathepsin H (Catalog # 7516-CY), and Recombinant Human Cathepsin F. PVDF Membrane was probed with 1 µg/mL of Rat Anti-Human/Mouse Cathepsin L Monoclonal Antibody (Catalog # MAB9521) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Cathepsin L at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3. For natural samples, we recommend the use of Catalog # AF1515.

Immunohistochemistry Cathepsin L antibody in Human Kidney by Immunohistochemistry (IHC-P). View Larger

Cathepsin L in Human Kidney. Cathepsin L was detected in immersion fixed paraffin-embedded sections of human kidney using Rat Anti-Human/Mouse Cathepsin L Monoclonal Antibody (Catalog # MAB9521) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in tubular epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Immunohistochemistry Cathepsin L antibody in Mouse Kidney by Immunohistochemistry (IHC-Fr). View Larger

Cathepsin L in Mouse Kidney. Cathepsin L was detected in immersion fixed frozen sections of mouse kidney using Rat Anti-Human/Mouse Cathepsin L Monoclonal Antibody (Catalog # MAB9521) at 8 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in tubular epithelial cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Cathepsin L

Cathepsin L is a lysosomal cysteine protease expressed in most eukaryotic cells. Cathepsin L is known to hydrolyze a number of proteins, including the proform of urokinase-type plasminogen activator, which is activated by Cathepsin L cleavage (1). Cathepsin L has also been shown to proteolytically inactivate alpha 1-antitrypsin and secretory leucoprotease inhibitor, two major protease inhibitors of the respiratory tract (2). These observations, combined with the demonstration of increased Cathepsin L activity in the epithelial lining fluid of the lungs of emphysema patients, have led to the suggestion that the enzyme may be involved in the progression of this disease. Cathepsin L has also been identified as a major excreted protein of transformed fibroblasts, indicating the enzyme could be involved in malignant tumor growth (3).

References
  1. Goretzki, L. et al. (1992) FEBS Lett. 297:112.
  2. Taggart, C.C. et al. (2001) J. Biol. Chem. 276:33345.
  3. Gottesman, M.M. and F. Cabral (1981) Biochemistry 20:1659.
Entrez Gene IDs
1514 (Human); 13039 (Mouse); 118276442 (Insect)
Alternate Names
Cathepsin L; cathepsin L1; CATL; CTSL; CTSL1; CTSLEC 3.4.22.15; EC 3.4.22; FLJ31037; Major excreted protein; MEP

Product Datasheets

Citations for Human/Mouse Cathepsin L Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
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  1. PLA2G4A/cPLA2-mediated lysosomal membrane damage leads to inhibition of autophagy and neurodegeneration after brain trauma
    Authors: C Sarkar, JW Jones, N Hegdekar, JA Thayer, A Kumar, AI Faden, MA Kane, MM Lipinski
    Autophagy, 2019;0(0):1-20.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  2. CCT complex restricts neuropathogenic protein aggregation via autophagy
    Nat Commun, 2016;7(0):13821.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. A C9ORF72/SMCR8-containing complex regulates ULK1 and plays a dual role in autophagy
    Sci Adv, 2016;2(9):e1601167.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. The PI3K regulatory subunits p55alpha and p50alpha regulate cell death in vivo.
    Authors: Pensa S, Neoh K, Resemann H, Kreuzaler P, Abell K, Clarke N, Reinheckel T, Kahn C, Watson C
    Cell Death Differ, 2014;21(9):1442-50.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  5. Macrophages are crucial for epithelial cell death and adipocyte repopulation during mammary gland involution.
    Authors: O'Brien J, Martinson H, Durand-Rougely C
    Development, 2012;139(2):269-75.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  6. Stat3 controls lysosomal-mediated cell death in vivo.
    Authors: Kreuzaler PA, Staniszewska AD, Li W, Omidvar N, Kedjouar B, Turkson J, Poli V, Flavell RA, Clarkson RW, Watson CJ
    Nat. Cell Biol., 2011;13(3):303-9.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  7. P2X(7) receptor-mediated release of cathepsins from macrophages is a cytokine-independent mechanism potentially involved in joint diseases.
    Authors: Lopez-Castejon G, Theaker J, Pelegrin P
    J. Immunol., 2010;185(4):2611-9.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  8. The cystatin M/E-cathepsin L balance is essential for tissue homeostasis in epidermis, hair follicles, and cornea.
    Authors: Zeeuwen PL, van Vlijmen-Willems IM, Cheng T
    FASEB J., 2010;24(10):3744-55.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  9. Primary tumour expression of the cysteine cathepsin inhibitor Stefin A inhibits distant metastasis in breast cancer.
    Authors: Parker BS, Ciocca DR, Bidwell BN, Gago FE, Fanelli MA, George J, Slavin JL, Moller A, Steel R, Pouliot N, Eckhardt BL, Henderson MA, Anderson RL
    J. Pathol., 2008;214(3):337-46.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  10. Cystatin M/E is a high affinity inhibitor of cathepsin V and cathepsin L by a reactive site that is distinct from the legumain-binding site. A novel clue for the role of cystatin M/E in epidermal cornification.
    Authors: Cheng T, Hitomi K, van Vlijmen-Willems IM, de Jongh GJ, Yamamoto K, Nishi K, Watts C, Reinheckel T, Schalkwijk J, Zeeuwen PL
    J. Biol. Chem., 2006;281(23):15893-9.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC

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Reviews for Human/Mouse Cathepsin L Antibody

Average Rating: 4.8 (Based on 4 Reviews)

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Human/Mouse Cathepsin L Antibody
By Anonymous on 07/09/2022
Application: Immunohistochemistry Sample Tested: Liver tissue Species: Mouse

Human/Mouse Cathepsin L Antibody
By Anonymous on 10/04/2021
Application: Immunohistochemistry Sample Tested: Ovarian follicles Species: Mouse

Human/Mouse Cathepsin L Antibody
By Anonymous on 03/17/2021
Application: Microarrays Sample Tested: EDTA Plasma Species: Human

Human/Mouse Cathepsin L Antibody
By Anonymous on 08/05/2020
Application: Immunohistochemistry Sample Tested: Kidney tissue Species: Mouse