Human/Mouse Cathepsin L Antibody

(9 citations)   
  • Species Reactivity
    Human, Mouse
  • Specificity
    Detects human and mouse Cathepsin L in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human Cathepsin B, C, L2, O, S, or X/Z/P is observed. In Western blots, both the pro and active forms of recombinant human and mouse Cathspsin L are recognized.
  • Source
    Monoclonal Rat IgG2A Clone # 204101
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human Cathepsin L
    Glu113-Val333
    Accession # P07711
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
  • Immunohistochemistry
    5-25 µg/mL
    See below
  • Immunoprecipitation
    25 µg/mL
    Conditioned cell culture medium spiked with Recombinant Human Cathepsin L (Catalog # 952-CY) or Recombinant Mouse Cathepsin L (Catalog # 1515-CY), see our available Western blot detection antibodies. This antibody did not immunoprecipitate the pro form of recombinant mouse Cathepsin L.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Recombinant Human and Mouse Cathepsin L by Western Blot. Western blot shows 100 ng of Recombinant Human Cathepsin L (Catalog # 952-CY), Recombinant Mouse Cathepsin L (Catalog # 1515-CY), Recombinant Human Cathepsin V (Catalog # 1080-CY), Recombinant Human Cathepsin K, Recombinant Human Cathepsin S (Catalog # 1183-CY), Recombinant Human Cathepsin H (Catalog # 7516-CY), and Recombinant Human Cathepsin F. PVDF Membrane was probed with 1 µg/mL of Rat Anti-Human/Mouse Cathepsin L Monoclonal Antibody (Catalog # MAB9521) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Cathepsin L at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.For natural samples, we recommend the use of Catalog # AF1515.
Immunohistochemistry
Cathepsin L in Human Kidney. Cathepsin L was detected in immersion fixed paraffin-embedded sections of human kidney using Rat Anti-Human/Mouse Cathepsin L Monoclonal Antibody (Catalog # MAB9521) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in tubular epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Immunohistochemistry
Cathepsin L in Mouse Kidney. Cathepsin L was detected in immersion fixed frozen sections of mouse kidney using Rat Anti-Human/Mouse Cathepsin L Monoclonal Antibody (Catalog # MAB9521) at 8 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in tubular epithelial cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Cathepsin L
Cathepsin L is a lysosomal cysteine protease expressed in most eukaryotic cells. Cathepsin L is known to hydrolyze a number of proteins, including the proform of urokinase-type plasminogen activator, which is activated by Cathepsin L cleavage (1). Cathepsin L has also been shown to proteolytically inactivate alpha 1-antitrypsin and secretory leucoprotease inhibitor, two major protease inhibitors of the respiratory tract (2). These observations, combined with the demonstration of increased Cathepsin L activity in the epithelial lining fluid of the lungs of emphysema patients, have led to the suggestion that the enzyme may be involved in the progression of this disease. Cathepsin L has also been identified as a major excreted protein of transformed fibroblasts, indicating the enzyme could be involved in malignant tumor growth (3).
  • References:
    1. Goretzki, L. et al. (1992) FEBS Lett. 297:112.
    2. Taggart, C.C. et al. (2001) J. Biol. Chem. 276:33345.
    3. Gottesman, M.M. and F. Cabral (1981) Biochemistry 20:1659.
  • Entrez Gene IDs:
    1514 (Human); 1514 (Human); 13039 (Mouse)
  • Alternate Names:
    Cathepsin L; cathepsin L1; CATL; CTSL; CTSL1; CTSLEC 3.4.22.15; EC 3.4.22; FLJ31037; Major excreted protein; MEP
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
Filter your results:

Species
Applications
Sample Type
  1. CCT complex restricts neuropathogenic protein aggregation via autophagy
    Nat Commun, 2016;7(0):13821.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  2. A C9ORF72/SMCR8-containing complex regulates ULK1 and plays a dual role in autophagy
    Sci Adv, 2016;2(9):e1601167.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  3. The PI3K regulatory subunits p55alpha and p50alpha regulate cell death in vivo.
    Authors: Pensa S, Neoh K, Resemann H, Kreuzaler P, Abell K, Clarke N, Reinheckel T, Kahn C, Watson C
    Cell Death Differ, 2014;21(9):1442-50.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  4. Macrophages are crucial for epithelial cell death and adipocyte repopulation during mammary gland involution.
    Authors: O'Brien J, Martinson H, Durand-Rougely C
    Development, 2012;139(2):269-75.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  5. Stat3 controls lysosomal-mediated cell death in vivo.
    Authors: Kreuzaler PA, Staniszewska AD, Li W, Omidvar N, Kedjouar B, Turkson J, Poli V, Flavell RA, Clarkson RW, Watson CJ
    Nat. Cell Biol., 2011;13(3):303-9.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  6. P2X(7) receptor-mediated release of cathepsins from macrophages is a cytokine-independent mechanism potentially involved in joint diseases.
    Authors: Lopez-Castejon G, Theaker J, Pelegrin P
    J. Immunol., 2010;185(4):2611-9.
    Species: Human
    Sample Type: Cell Culture Supernates
    Application: WB
  7. The cystatin M/E-cathepsin L balance is essential for tissue homeostasis in epidermis, hair follicles, and cornea.
    Authors: Zeeuwen PL, van Vlijmen-Willems IM, Cheng T
    FASEB J., 2010;24(10):3744-55.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  8. Primary tumour expression of the cysteine cathepsin inhibitor Stefin A inhibits distant metastasis in breast cancer.
    Authors: Parker BS, Ciocca DR, Bidwell BN, Gago FE, Fanelli MA, George J, Slavin JL, Moller A, Steel R, Pouliot N, Eckhardt BL, Henderson MA, Anderson RL
    J. Pathol., 2008;214(3):337-46.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  9. Cystatin M/E is a high affinity inhibitor of cathepsin V and cathepsin L by a reactive site that is distinct from the legumain-binding site. A novel clue for the role of cystatin M/E in epidermal cornification.
    Authors: Cheng T, Hitomi K, van Vlijmen-Willems IM, de Jongh GJ, Yamamoto K, Nishi K, Watts C, Reinheckel T, Schalkwijk J, Zeeuwen PL
    J. Biol. Chem., 2006;281(23):15893-9.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC
Cell and Tissue Staining Kits
Description Application Cat# Citations Images  

Anti-Rat HRP-DAB Cell & Tissue Staining Kit

CTS017 5
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
ExactaChIP Chromatin Immunoprecipitation Kits
Description Application Cat# Citations Images  

ExactaChIP Chromatin IP Buffer Panel

ChIP ECP001 2
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Immunohistochemistry Reagents
Description Application Cat# Citations Images  

Antigen Retrieval Reagent-Universal

CTS015 10
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Antigen Retrieval Reagent-Basic

CTS013 4
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Mounting Medium

CTS011 4
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Antigen Retrieval Reagent Sampler (50 mL each)

CTS016
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Antigen Retrieval Reagent-Acidic

CTS014
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

NorthernLights Guard Mounting Media

NL996
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

VisUCyte Antigen Retrieval Reagent-Acidic

VCTS022
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

VisUCyte Antigen Retrieval Reagent-Basic

VCTS021
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

VisUCyte Antigen Retrieval Reagent-Universal

VCTS023
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Isotype Controls
Description Application Cat# Citations Images  

Rat IgG2A Isotype Control

Ctrl MAB006 90  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Normal Rat IgG Control

Ctrl 6-001-A 9
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Normal Rat IgG Control (Azide Free)

Ctrl 6-001-F 1
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Rat IgG2A Isotype Control

Ctrl MAB006R  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Secondary Antibodies
Description Application Cat# Citations Images  

Rat IgG HRP-conjugated Antibody

WB, Simple Western HAF005 7  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat Anti-Rat IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL013 2
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat Anti-Rat F(ab)2 IgG (H+L)
APC-conjugated Antibody

Flow F0113 2  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Rat F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0105B 2  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat Anti-Rat IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL015 1
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat Anti-Rat IgG Biotinylated Antibody

WB BAF005 2
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Rat IgG VisUCyte HRP Polymer Antibody

IHC VC005  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat Anti-Rat IgG Antibody

WB AF005 1
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Rat F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0104B  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat Anti-Rat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL014
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat Anti-Rat F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0115 1
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Have you used Human/Mouse Cathepsin L Antibody?

Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

Order Details

Contact Information

Calculators

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷
R&D Systems Guarantee

Monoclonal
Antibody
Panels For
Screening

Learn More
Monoclonal Antibodies

Feedback

Customer Information