Mouse/Rat Cathepsin L Antibody

(11 citations)
(1 Review)
  
  • Species Reactivity
    Mouse, Rat
  • Specificity
    Detects mouse Cathepsin L in direct ELISAs and Western blots. In Western blots, approximately 25% cross-reactivity with recombinant human Cathepsin L is observed, and less than 1% cross-reactivity with recombinant mouse (rm) Cathepsin A, rmCathepsin B, rmCathepsin C, rmCathepsin D, rmCathepsin E, rmCathepsin H, rmCathepsin X/Z/P is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse Cathepsin L
    Thr18-Asn334
    Accession # P06797
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
  • Simple Western
    10 µg/mL
    See below
  • Immunohistochemistry
    5-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Mouse and Rat Cathepsin L by Western Blot. Western blot shows lysates of rat liver tissue, mouse liver tissue (wild type), and mouse liver tissue (knock out). PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse Cathepsin L Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1515) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for Cathepsin L at approximately 22-38 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunohistochemistry
Cathepsin L in Mouse Ovary. Cathepsin L was detected in perfusion fixed frozen sections of mouse ovary using 15 µg/mL Goat Anti-Mouse Cathepsin L Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1515) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Immunohistochemistry
Cathepsin L in Mouse Thymus. Cathepsin L was detected in perfusion fixed frozen sections of mouse thymus using 15 µg/mL Goat Anti-Mouse Cathepsin L Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1515) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS0028) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Detection of Mouse Cathepsin L by Simple WesternTM. Simple Western lane view shows lysates of mouse liver tissue and HepG2 human hepatocellular carcinoma cell line, loaded at 0.2 mg/mL. Specific bands were detected for Cathepsin L at approximately 51 and 34 kDa (as indicated) using 10 µg/mL of Goat Anti-Mouse Cathepsin L Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1515) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody
(Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.        
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Cathepsin L

Cathepsin L is a lysosomal cysteine protease expressed in most eukaryotic cells. Cathepsin L is known to hydrolyze a number of proteins, including the proform of urokinase-type plasminogen activator, which is activated by Cathepsin L cleavage (1). Cathepsin L has also been shown to proteolytically inactivate alpha 1-antitrypsin and secretory leucoprotease inhibitor, two major protease inhibitors of the respiratory tract (2). These observations, combined with the demonstration of increased Cathepsin L activity in the epithelial lining fluid of the lungs of emphysema patients, have led to the suggestion that the enzyme may be involved in the progression of this disease. Cathepsin L has also been identified as a major excreted protein of transformed fibroblasts, indicating the enzyme could be involved in malignant tumor growth (3). In Cathepsin L-deficient mice, it appears to play a critical role in cardiac morphology and function, epidermal homeostasis, regulation of the hair cycle, and MHC class II-mediated antigen presentation in cortical epithelial cells of the thymus (4, 5). Mouse Cathepsin L is synthesized as a 334 amino acid precursor with a signal peptide (residues 1-17), a pro region (residues 18-113), and a mature chain (residues 114-334).

  • References:
    1. Goretzki, L. et al. (1992) FEBS Lett. 297:112.
    2. Taggart, C.C. et al. (2001) J. Biol. Chem. 276:33345.
    3. Gottesman, M.M. and F. Cabral (1981) Biochemistry 20:1659.
    4. Stypmann, J. et al. (2002) Proc. Natl. Acad. Sci. USA 99: 6234.
    5. Reinheckel, T. et al. (2001) Biol. Chem. 382:735.
  • Entrez Gene IDs:
    1514 (Human); 1514 (Human); 13039 (Mouse)
  • Alternate Names:
    Cathepsin L; cathepsin L1; CATL; CTSL; CTSL1; CTSLEC 3.4.22.15; EC 3.4.22; FLJ31037; Major excreted protein; MEP
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

11 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. Direct Observation of Enhanced Nitric Oxide in a Murine Model of Diabetic Nephropathy
    Authors: MG Boels, EE van Faasse, MC Avramut, J van der Vl, BM van den Be, TJ Rabelink
    PLoS ONE, 2017;12(1):e0170065.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC - Paraffin embedded
  2. The Role of Heparanase in the Pathogenesis of Acute Pancreatitis: A Potential Therapeutic Target
    Authors: I Khamaysi, P Singh, S Nasser, H Awad, Y Chowers, E Sabo, E Hammond, I Gralnek, I Minkov, A Noseda, N Ilan, I Vlodavsky, Z Abassi
    Sci Rep, 2017;7(1):715.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  3. Investigating the Life Expectancy and Proteolytic Degradation of Engineered Skeletal Muscle Biological Machines
    Authors: C Cvetkovic, MC Ferrall-Fa, E Ko, L Grant, H Kong, MO Platt, R Bashir
    Sci Rep, 2017;7(1):3775.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  4. Lysosomal protein turnover contributes to the acquisition of TGFbeta-1 induced invasive properties of mammary cancer cells.
    Authors: Kern U, Wischnewski V, Biniossek M, Schilling O, Reinheckel T
    Mol Cancer, 2015;14(0):39.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  5. Deficiency for the cysteine protease cathepsin L impairs Myc-induced tumorigenesis in a mouse model of pancreatic neuroendocrine cancer.
    Authors: Brindle N, Joyce J, Rostker F, Lawlor E, Swigart-Brown L, Evan G, Hanahan D, Shchors K
    PLoS ONE, 2015;10(4):e0120348.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC-OCT embedded
  6. Vacuolar ATPase in phagosome-lysosome fusion.
    Authors: Kissing S, Hermsen C, Repnik U, Nesset C, von Bargen K, Griffiths G, Ichihara A, Lee B, Schwake M, De Brabander J, Haas A, Saftig P
    J Biol Chem, 2015;290(22):14166-80.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  7. The PI3K regulatory subunits p55alpha and p50alpha regulate cell death in vivo.
    Authors: Pensa S, Neoh K, Resemann H, Kreuzaler P, Abell K, Clarke N, Reinheckel T, Kahn C, Watson C
    Cell Death Differ, 2014;21(9):1442-50.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  8. Biogenesis and proteolytic processing of lysosomal DNase II.
    Authors: Ohkouchi, Susumu, Shibata, Masahiro, Sasaki, Mitsuho, Koike, Masato, Safig, Paul, Peters, Christop, Nagata, Shigekaz, Uchiyama, Yasuo
    PLoS ONE, 2013;8(3):e59148.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  9. Macrophages and cathepsin proteases blunt chemotherapeutic response in breast cancer.
    Authors: Shree T, Olson OC, Elie BT
    Genes Dev., 2011;25(23):2465-79.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  10. Major Role of Cathepsin L for Producing the Peptide Hormones ACTH, beta-Endorphin, and alpha-MSH, Illustrated by Protease Gene Knockout and Expression.
    Authors: Funkelstein L, Toneff T, Mosier C, Hwang SR, Beuschlein F, Lichtenauer UD, Reinheckel T, Peters C, Hook V
    J. Biol. Chem., 2008;283(51):35652-9.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Fresh
  11. The impact of microRNAs on protein output.
    Authors: Baek D, Villen J, Shin C, Camargo FD, Gygi SP, Bartel DP
    Nature, 2008;455(7209):64-71.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
Expand to show all 11 Citations
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Isotype Controls
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Average Rating: 5 (Based on 1 review)

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We have 1 review tested in 1 application: Western Blot.

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Images Ratings Applications Species Reviewed By Date Details
Western Blot Mouse/Rat Cathepsin L Antibody AF1515
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 WB Mouse Anonymous 05/03/2017
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Western Blot Mouse/Rat Cathepsin L Antibody AF1515
Western Blot: Mouse/Rat Cathepsin L Antibody [AF1515]

Summary

ApplicationWestern Blot
Sample Testedmouse feces
SpeciesMouse

Other Experimental Details

Other Experimental DetailsAntibody in 1% milk (TBST). Incubation time was 2 hrs @ RT.Detection in mouse feces.

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