Mouse/Rat Cathepsin L Antibody

Catalog # Availability Size / Price Qty
AF1515
AF1515-SP
Detection of Mouse and Rat Cathepsin L by Western Blot.
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Product Details
Citations (22)
FAQs
Supplemental Products
Reviews (3)

Mouse/Rat Cathepsin L Antibody Summary

Species Reactivity
Mouse, Rat
Specificity
Detects mouse Cathepsin L in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Cathepsin L
Thr18-Asn334
Accession # P06797
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Simple Western
10 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Mouse and Rat Cathepsin L antibody by Western Blot. View Larger

Detection of Mouse and Rat Cathepsin L by Western Blot. Western blot shows lysates of rat liver tissue, mouse liver tissue (wild type), and mouse liver tissue (knock out). PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse Cathepsin L Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1515) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). Specific bands were detected for Cathepsin L at approximately 22-38 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry Cathepsin L antibody in Mouse Ovary by Immunohistochemistry (IHC-Fr). View Larger

Cathepsin L in Mouse Ovary. Cathepsin L was detected in perfusion fixed frozen sections of mouse ovary using 15 µg/mL Goat Anti-Mouse Cathepsin L Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1515) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Immunohistochemistry Cathepsin L antibody in Mouse Thymus by Immunohistochemistry (IHC-Fr). View Larger

Cathepsin L in Mouse Thymus. Cathepsin L was detected in perfusion fixed frozen sections of mouse thymus using 15 µg/mL Goat Anti-Mouse Cathepsin L Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1515) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Simple Western Detection of Mouse Cathepsin L antibody by Simple Western<sup>TM</sup>. View Larger

Detection of Mouse Cathepsin L by Simple WesternTM. Simple Western lane view shows lysates of mouse liver tissue and HepG2 human hepatocellular carcinoma cell line, loaded at 0.2 mg/mL. Specific bands were detected for Cathepsin L at approximately 51 and 34 kDa (as indicated) using 10 µg/mL of Goat Anti-Mouse Cathepsin L Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1515) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Cathepsin L

Cathepsin L is a lysosomal cysteine protease expressed in most eukaryotic cells. Cathepsin L is known to hydrolyze a number of proteins, including the proform of urokinase-type plasminogen activator, which is activated by Cathepsin L cleavage (1). Cathepsin L has also been shown to proteolytically inactivate alpha 1-antitrypsin and secretory leucoprotease inhibitor, two major protease inhibitors of the respiratory tract (2). These observations, combined with the demonstration of increased Cathepsin L activity in the epithelial lining fluid of the lungs of emphysema patients, have led to the suggestion that the enzyme may be involved in the progression of this disease. Cathepsin L has also been identified as a major excreted protein of transformed fibroblasts, indicating the enzyme could be involved in malignant tumor growth (3). In Cathepsin L-deficient mice, it appears to play a critical role in cardiac morphology and function, epidermal homeostasis, regulation of the hair cycle, and MHC class II-mediated antigen presentation in cortical epithelial cells of the thymus (4, 5). Mouse Cathepsin L is synthesized as a 334 amino acid precursor with a signal peptide (residues 1-17), a pro region (residues 18-113), and a mature chain (residues 114-334).

References
  1. Goretzki, L. et al. (1992) FEBS Lett. 297:112.
  2. Taggart, C.C. et al. (2001) J. Biol. Chem. 276:33345.
  3. Gottesman, M.M. and F. Cabral (1981) Biochemistry 20:1659.
  4. Stypmann, J. et al. (2002) Proc. Natl. Acad. Sci. USA 99: 6234.
  5. Reinheckel, T. et al. (2001) Biol. Chem. 382:735.
Entrez Gene IDs
1514 (Human); 13039 (Mouse); 118276442 (Insect)
Alternate Names
Cathepsin L; cathepsin L1; CATL; CTSL; CTSL1; CTSLEC 3.4.22.15; EC 3.4.22; FLJ31037; Major excreted protein; MEP

Product Datasheets

Citations for Mouse/Rat Cathepsin L Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

22 Citations: Showing 1 - 10
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  1. TMEM106B deficiency impairs cerebellar myelination and synaptic integrity with Purkinje cell loss
    Authors: T Feng, L Luan, II Katz, M Ullah, VM Van Deerli, JQ Trojanowsk, EB Lee, F Hu
    Acta neuropathologica communications, 2022;10(1):33.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  2. Chitinase 3-like-1 is a Therapeutic Target That Mediates the Effects of Aging in COVID-19
    Authors: S Kamle, B Ma, CH He, B Akosman, Y Zhou, CM Lee, WS El-Deiry, K Huntington, O Liang, J Machan, MJ Kang, HJ Shin, E Mizoguchi, CG Lee, JA Elias
    bioRxiv : the preprint server for biology, 2021;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  3. Translation Inhibitors Activate Autophagy Master Regulators TFEB and TFE3
    Authors: TT Dang, SH Back
    International Journal of Molecular Sciences, 2021;22(21):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blots
  4. Cathepsin L regulates pathogenicCD4 T cells in experimental autoimmune encephalomyelitis
    Authors: M Shibamura-, K Yuki, L Hou
    International immunopharmacology, 2021;93(0):107425.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  5. Conditional Gene Targeting Reveals Cell Type-Specific Roles of the Lysosomal Protease Cathepsin L in Mammary Tumor Progression
    Authors: MA Parigiani, A Ketscher, S Timme, P Bronsert, M Schlimpert, B Kammerer, A Jacquel, P Chaintreui, T Reinheckel
    Cancers (Basel), 2020;12(8):.
    Species: Mouse
    Sample Types: Cell Culture Lysates
    Applications: Western Blot
  6. Imbalanced cellular metabolism compromises cartilage homeostasis and joint function in a mouse model of mucolipidosis type III gamma
    Authors: LM Westermann, L Fleischhau, J Vogel, Z Jenei-Lanz, N Floriano L, L Schau, F Morellini, A Baranowsky, TA Yorgan, G Di Lorenzo, M Schweizer, B de Souza P, NR Guarany, F Sperb-Ludw, F Visioli, T Oliveira S, J Soul, G Hendrickx, JS Wiegert, IVD Schwartz, H Clausen-Sc, F Zaucke, T Schinke, S Pohl, T Danyukova
    Dis Model Mech, 2020;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  7. Cathepsin D deficiency in mammary epithelium transiently stalls breast cancer by interference with mTORC1 signaling
    Authors: S Ketterer, J Mitschke, A Ketscher, M Schlimpert, W Reichardt, N Baeuerle, ME Hess, P Metzger, M Boerries, C Peters, B Kammerer, T Brummer, F Steinberg, T Reinheckel
    Nat Commun, 2020;11(1):5133.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  8. PLA2G4A/cPLA2-mediated lysosomal membrane damage leads to inhibition of autophagy and neurodegeneration after brain trauma
    Authors: C Sarkar, JW Jones, N Hegdekar, JA Thayer, A Kumar, AI Faden, MA Kane, MM Lipinski
    Autophagy, 2019;0(0):1-20.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  9. Sequential, but not Concurrent, Incubation of Cathepsin K and L with Type I Collagen Results in Extended Proteolysis
    Authors: AN Parks, J Nahata, NE Edouard, JS Temenoff, MO Platt
    Sci Rep, 2019;9(1):5399.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Western Blot
  10. Stat3 mediated alterations in lysosomal membrane protein composition
    Authors: B Lloyd-Lewi, CC Krueger, TJ Sargeant, ME D'Angelo, MJ Deery, R Feret, JA Howard, KS Lilley, CJ Watson
    J. Biol. Chem., 2018;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  11. Early lysosomal maturation deficits in microglia triggers enhanced lysosomal activity in other brain cells of progranulin knockout mice
    Authors: JK Götzl, AV Colombo, K Fellerer, A Reifschnei, G Werner, S Tahirovic, C Haass, A Capell
    Mol Neurodegener, 2018;13(1):48.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  12. The Role of Heparanase in the Pathogenesis of Acute Pancreatitis: A Potential Therapeutic Target
    Authors: I Khamaysi, P Singh, S Nasser, H Awad, Y Chowers, E Sabo, E Hammond, I Gralnek, I Minkov, A Noseda, N Ilan, I Vlodavsky, Z Abassi
    Sci Rep, 2017;7(1):715.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  13. Investigating the Life Expectancy and Proteolytic Degradation of Engineered Skeletal Muscle Biological Machines
    Authors: C Cvetkovic, MC Ferrall-Fa, E Ko, L Grant, H Kong, MO Platt, R Bashir
    Sci Rep, 2017;7(1):3775.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  14. Direct Observation of Enhanced Nitric Oxide in a Murine Model of Diabetic Nephropathy
    Authors: MG Boels, EE van Faasse, MC Avramut, J van der Vl, BM van den Be, TJ Rabelink
    PLoS ONE, 2017;12(1):e0170065.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  15. Vacuolar ATPase in phagosome-lysosome fusion.
    Authors: Kissing S, Hermsen C, Repnik U, Nesset C, von Bargen K, Griffiths G, Ichihara A, Lee B, Schwake M, De Brabander J, Haas A, Saftig P
    J Biol Chem, 2015;290(22):14166-80.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  16. Lysosomal protein turnover contributes to the acquisition of TGFbeta-1 induced invasive properties of mammary cancer cells.
    Authors: Kern U, Wischnewski V, Biniossek M, Schilling O, Reinheckel T
    Mol Cancer, 2015;14(0):39.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  17. Deficiency for the cysteine protease cathepsin L impairs Myc-induced tumorigenesis in a mouse model of pancreatic neuroendocrine cancer.
    Authors: Brindle N, Joyce J, Rostker F, Lawlor E, Swigart-Brown L, Evan G, Hanahan D, Shchors K
    PLoS ONE, 2015;10(4):e0120348.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  18. The PI3K regulatory subunits p55alpha and p50alpha regulate cell death in vivo.
    Authors: Pensa S, Neoh K, Resemann H, Kreuzaler P, Abell K, Clarke N, Reinheckel T, Kahn C, Watson C
    Cell Death Differ, 2014;21(9):1442-50.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  19. Biogenesis and proteolytic processing of lysosomal DNase II.
    Authors: Ohkouchi, Susumu, Shibata, Masahiro, Sasaki, Mitsuho, Koike, Masato, Safig, Paul, Peters, Christop, Nagata, Shigekaz, Uchiyama, Yasuo
    PLoS ONE, 2013;8(3):e59148.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  20. Macrophages and cathepsin proteases blunt chemotherapeutic response in breast cancer.
    Authors: Shree T, Olson OC, Elie BT
    Genes Dev., 2011;25(23):2465-79.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  21. The impact of microRNAs on protein output.
    Authors: Baek D, Villen J, Shin C, Camargo FD, Gygi SP, Bartel DP
    Nature, 2008;455(7209):64-71.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  22. Major Role of Cathepsin L for Producing the Peptide Hormones ACTH, beta-Endorphin, and alpha-MSH, Illustrated by Protease Gene Knockout and Expression.
    Authors: Funkelstein L, Toneff T, Mosier C, Hwang SR, Beuschlein F, Lichtenauer UD, Reinheckel T, Peters C, Hook V
    J. Biol. Chem., 2008;283(51):35652-9.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr

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Reviews for Mouse/Rat Cathepsin L Antibody

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Mouse/Rat Cathepsin L Antibody
By Anonymous on 12/06/2021
Application: Immunocytochemistry/Immunofluorescence Sample Tested: bone marrow macrophage Species: Mouse

Mouse/Rat Cathepsin L Antibody
By Anonymous on 10/17/2018
Application: Western Blot Sample Tested: H4-II-E-C3 rat hepatoma cell line,Hepa 1-6 mouse hepatoma cell line Species: Mouse and Rat

Mouse/Rat Cathepsin L Antibody
By Gonzalo Gonzalez on 05/03/2017
Application: Western Blot Sample Tested: mouse feces Species: Mouse

Antibody in 1% milk (TBST). Incubation time was 2 hrs @ RT.
Detection in mouse feces.