Human/Mouse CBL Antibody

Catalog # Availability Size / Price Qty
AF5998
AF5998-SP

Save Up to 40% on RUO Reagents with BIOSPRING24 (See Details)

Detection of Human CBL by Western Blot.
2 Images
Product Details
Citations (1)
FAQs
Supplemental Products
Reviews

Human/Mouse CBL Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human and mouse CBL in Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human CBL
Asp775-Thr906
Accession # P22681
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below
Simple Western
10 µg/mL
Daudi human Burkitt's lymphoma cells, K562 human chronic myelogenous leukemia cells, Raji human Burkitt's lymphoma cells

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human CBL antibody by Western Blot. View Larger

Detection of Human CBL by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line, K562 human chronic myelogenous leukemia cell line, and Raji human Burkitt's lymphoma cell line. PVDF Membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse CBL Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5998) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF109). A specific band was detected for CBL at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western View Larger

Detection of Human/Mouse CBL by Simple WesternTM. Simple Western lane view shows lysates of Daudi human Burkitt's lymphoma cells, K562 human chronic myelogenous leukemia cells, and Raji human Burkitt's lymphoma cells, loaded at 0.2 mg/mL. A specific band was detected for CBL at approximately 116 kDa (as indicated) using 10 µg/mL of Goat Anti-Human/Mouse CBL Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5998). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Loading...
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CBL

CBL (Lake Casitas, California mouse B-lineage Lymphoma; also RING finger protein 55) is a 120‑125 kDa cytosolic member of the CBL family of proteins. It is widely expressed, being found in cells such as fibroblasts, adipocytes, osteoclasts, podocytes and mammary epithelium. CBL negatively regulates RTK signaling by acting as an E3 ubiquitin ligase. Following activation via phosphorylation, CBL will interact with multiple signaling molecules such as Src, ZAP-70, EGFR and SHP1. Human CBL is 906 amino acids (aa) in length. It contains an N-terminal phosphoTyr-binding region that consists of one alpha -helix bundle, an EF hand segment, and an SH2 domain (aa 47‑342), a RING finger domain that interacts with E2 enzymes (aa 381‑423) a Pro-rich SH3 domain (aa 477‑688), and a Leu‑zipper/Ubiquitin‑associated region (aa 861‑892). There is one potential alternative start site at Met55. Over aa 775‑906, human CBL shares 91% aa identity with mouse CBL.

Long Name
Casitas B-lineage Lymphoma, E3 Ubiquitin Protein Ligase CBL
Entrez Gene IDs
867 (Human); 12402 (Mouse); 500985 (Rat)
Alternate Names
Cas-Br-M (murine) ecotropic retroviral transforming sequence; Casitas B-lineage lymphoma proto-oncogene; CBL; CBL2; CBL2E3 ubiquitin-protein ligase CBL; EC 6.3.2; EC 6.3.2.-; oncogene CBL2; Proto-oncogene c-Cbl; RING finger protein 55; RNF55; RNF55C-CBL; Signal transduction protein CBL

Product Datasheets

You must select a language.

x

Citation for Human/Mouse CBL Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Metastasis suppressor tetraspanin CD82/KAI1 regulates ubiquitylation of epidermal growth factor receptor.
    Authors: Odintsova E, van Niel G, Conjeaud H, Raposo G, Iwamoto R, Mekada E, Berditchevski F
    J Biol Chem, 2013-07-29;288(36):26323-34.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs
Loading...

Reviews for Human/Mouse CBL Antibody

There are currently no reviews for this product. Be the first to review Human/Mouse CBL Antibody and earn rewards!

Have you used Human/Mouse CBL Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review