CLEC10A/CD301 Antibody

R&D Systems | Catalog # AF4888

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Transgenic Mouse

Applications

Validated:

Western Blot, Flow Cytometry, CyTOF-ready

Cited:

Immunohistochemistry, Flow Cytometry, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all CLEC10A/CD301 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human CLEC10A/CD301
Gln61-His316
Accession # Q8IUN9

Specificity

Detects human CLEC10A/CD301 in direct ELISAs and Western blots and mouse CLEC10A in Western blots. In this format, less than 1% cross-reactivity with recombinant human (rh) CLEC1, rhCLEC2, and rhCLEC3B is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for CLEC10A/CD301 Antibody

Detection of Human CLEC10A/CD301 antibody by Western Blot.

Detection of Human CLEC10A/CD301 by Western Blot.

Western blot shows lysates of human immature dendritic cells and human mature dendritic cells. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse CLEC10A/CD301 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4888) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for CLEC10A/CD301 at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of CLEC10A antibody in Human Dendritic Cells antibody by Flow Cytometry.

Detection of CLEC10A in Human Dendritic Cells by Flow Cytometry.

Human immature dendritic cells were stained with Goat Anti-Human/Mouse CLEC10A/CD301 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4888, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108).
Detection of Mouse CLEC10A/CD301 antibody by Western Blot.

Detection of Mouse CLEC10A/CD301 by Western Blot.

Western blot shows lysates of mouse immature dendritic cells and mouse mature dendritic cells. PVDF membrane was probed with 2 µg/mL of Goat Anti-Human/Mouse CLEC10A/CD301 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4888) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for CLEC10A/CD301 at approximately 37 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of CLEC10A/CD301 by Immunohistochemistry

Detection of CLEC10A/CD301 by Immunohistochemistry

C1GalT suppression reduces tumour growth in chicken embryos and more macrophages and dendritic cells are accumulated in the tumour microenvironment of ME C1galt1-/-/Erb mice than of C1galt1f/f/Erb mice.GFP-SW620shC1GT and GFP-SW620shCon cells (1.8 × 106 cells) without or with 5 µg galectin-3 was implanted in the CAMs at Day 7 and tumour sizes were measured at Day 14 of embryonation. Representative images of the tumours grown on CAM at Day 14 are shown in (a). Tumour volume are shown in (b) (number of eggs with detectable tumour/total number of eggs at Day 14 are also shown on top of each bar). Data are expressed as mean ± SD, *p < 0.05, **p < 0.01 (T test). c, d Fewer macrophages and dendritic cells were seen in the tumour microenvironment of C1galt1f/f/Erb mice than in ME C1galt1-/-/Erb mice. Lower expression of TF and higher expression of Tn were found in the tumours of ME C1galt1-/-/Erb mice compared with C1galt1f/f/Erb mice. Higher number of macrophages (F4/80 positive, MGL-positive) and dendritic cells (CD11c positive, MGL positive) are seen in the tumours of ME C1galt1-/-/Erb mice compared with C1galt1f/f/Erb mice. Two mice in each group were randomly selected and analyzed and representative images are shown. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37612278), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for CLEC10A/CD301 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/106 cells
Sample: Human immature dendritic cells

Western Blot

1 µg/mL
Sample: Human immature dendritic cells and human mature dendritic cells

Flow Cytometry Panel Builder

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Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CLEC10A/CD301

CLEC10A, also known as macrophage galactose/N-acetyl-galactosamine (GalNAc) specific lectin (MGL), CD301, DC-ASGPR, and HML, is a 35-40 kDa type II transmembrane glycoprotein that belongs to the C-type lectin family (1). Human and rat carry a single gene for CLEC10A/MGL, while mouse has two closely related MGL1 and MGL2 genes. Human CLEC10A/MGL consists of a 39 amino acid (aa) cytoplasmic region, a 21 aa transmembrane segment and a 256 aa extracellular domain (ECD) with one carbohydrate recognition domain (CRD) and a neck region (2). Within the CRD, human CLEC10A/MGL shares 64% - 70% aa sequence identity with mouse MGL1, mouse MGL2, and rat MGL. Alternate splicing generates multiple isoforms of human CLEC10A/MGL with 27 aa, 3 aa, and/or 4 aa deletions within the ECD (3, 4). CLEC10A/MGL is expressed on immature myleloid dendritic cells and alternatively activated (tolerogenic) macrophages and is upregulated by the immunosuppressant dexamethasone (3-7). CLEC10A/MGL selectively binds and internalizes terminal nonsialylated alpha - or beta -linked GalNAc moieties on O-linked carbohydrates, including the Tn carcinoma antigen (2-4, 8, 9). Similar ligand preference is exhibited by mouse MGL2 but not MGL1 (10). CLEC10A/MGL expressed on tolerogenic dendritic cells binds carbohydrate determinants on CD45 (RA, RB, and RC but not RO isoforms) expressed by T, NK, and B cells (6). This interaction inhibits effector T cell activation and induces their apoptosis (6). CLEC10A/MGL also binds the GP envelope glycoprotein on Marburg and Ebola viruses and enhances viral entry and infectivity (11).

References

  1. Zelensky, A.N. and J.E. Gready (2005) FEBS J. 272:6179.
  2. Suzuki, N. et al. (1996) J. Immunol. 156:128. 
  3. Higashi, N. et al. (2002) J. Biol. Chem. 277:20686. 
  4. Valladeau, J. et al. (2001) J. Immunol. 167:5767.
  5. van Vliet, S.J. et al. (2006) Immunobiology 211:577. 
  6. van Vliet, S.J. et al. (2006) Nat. Immunol. 7:1200.
  7. Raes, G. et al. (2005) J. Leukoc. Biol. 77:321.
  8. van Vliet, S.J. et al. (2005) Int. Immunol. 17:661.
  9. Higashi, N. et al. (2002) Int. Immunol. 14:545.
  10. Tsuiji, M. et al. (2002) J. Biol. Chem. 277:28892.
  11. Takada, A. et al. (2004) J. Virol. 78:2943.

Long Name

Macrophage Galactose N-Acetyl-Galactosamine Specific Lectin 1

Alternate Names

CD301, CLECSF14, HML2, MGL

Entrez Gene IDs

10462 (Human)

Gene Symbol

CLEC10A

UniProt

Q8IUN9 (Human)

Additional CLEC10A/CD301 Products

Product Documents for CLEC10A/CD301 Antibody

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Product Specific Notices for CLEC10A/CD301 Antibody

For research use only

Citations for CLEC10A/CD301 Antibody

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