Human/Mouse CLEC10A/CD301 Antibody

  
  • Species Reactivity
    Human, Mouse
  • Specificity
    Detects human CLEC10A/CD301 in direct ELISAs and Western blots and mouse CLEC10A in Western blots. In this format, less than 1% cross-reactivity with recombinant human (rh) CLEC1, rhCLEC2, and rhCLEC3B is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human CLEC10A/CD301
    Gln61-His316
    Accession # Q8IUN9
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
  • Flow Cytometry
    2.5 µg/106 cells
    See below
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human CLEC10A/CD301 by Western Blot. Western blot shows lysates of human immature dendritic cells and human mature dendritic cells. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse CLEC10A/CD301 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4888) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for CLEC10A/CD301 at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of CLEC10A in Human Dendritic Cells by Flow Cytometry. Human immature dendritic cells were stained with Goat Anti-Human/Mouse CLEC10A/CD301 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4888, filled histogram) or isotype control antibody (Catalog # AB‑108‑C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108).
Detection of Mouse CLEC10A/CD301 by Western Blot. Western blot shows lysates of mouse immature dendritic cells and mouse mature dendritic cells. PVDF membrane was probed with 2 µg/mL of Goat Anti-Human/Mouse CLEC10A/CD301 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4888) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for CLEC10A/CD301 at approximately 37 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CLEC10A/CD301

CLEC10A, also known as macrophage galactose/N-acetyl-galactosamine (GalNAc) specific lectin (MGL), CD301, DC-ASGPR, and HML, is a 35-40 kDa type II transmembrane glycoprotein that belongs to the C-type lectin family (1). Human and rat carry a single gene for CLEC10A/MGL, while mouse has two closely related MGL1 and MGL2 genes. Human CLEC10A/MGL consists of a 39 amino acid (aa) cytoplasmic region, a 21 aa transmembrane segment and a 256 aa extracellular domain (ECD) with one carbohydrate recognition domain (CRD) and a neck region (2). Within the CRD, human CLEC10A/MGL shares 64% - 70% aa sequence identity with mouse MGL1, mouse MGL2, and rat MGL. Alternate splicing generates multiple isoforms of human CLEC10A/MGL with 27 aa, 3 aa, and/or 4 aa deletions within the ECD (3, 4). CLEC10A/MGL is expressed on immature myleloid dendritic cells and alternatively activated (tolerogenic) macrophages and is upregulated by the immunosuppressant dexamethasone (3-7). CLEC10A/MGL selectively binds and internalizes terminal nonsialylated alpha - or beta -linked GalNAc moieties on O-linked carbohydrates, including the Tn carcinoma antigen (2-4, 8, 9). Similar ligand preference is exhibited by mouse MGL2 but not MGL1 (10). CLEC10A/MGL expressed on tolerogenic dendritic cells binds carbohydrate determinants on CD45 (RA, RB, and RC but not RO isoforms) expressed by T, NK, and B cells (6). This interaction inhibits effector T cell activation and induces their apoptosis (6). CLEC10A/MGL also binds the GP envelope glycoprotein on Marburg and Ebola viruses and enhances viral entry and infectivity (11).

  • References:
    1. Zelensky, A.N. and J.E. Gready (2005) FEBS J. 272:6179.
    2. Suzuki, N. et al. (1996) J. Immunol. 156:128. 
    3. Higashi, N. et al. (2002) J. Biol. Chem. 277:20686. 
    4. Valladeau, J. et al. (2001) J. Immunol. 167:5767.
    5. van Vliet, S.J. et al. (2006) Immunobiology 211:577. 
    6. van Vliet, S.J. et al. (2006) Nat. Immunol. 7:1200.
    7. Raes, G. et al. (2005) J. Leukoc. Biol. 77:321.
    8. van Vliet, S.J. et al. (2005) Int. Immunol. 17:661.
    9. Higashi, N. et al. (2002) Int. Immunol. 14:545.
    10. Tsuiji, M. et al. (2002) J. Biol. Chem. 277:28892.
    11. Takada, A. et al. (2004) J. Virol. 78:2943.
  • Long Name:
    Macrophage Galactose N-Acetyl-Galactosamine Specific Lectin 1
  • Entrez Gene IDs:
    10462 (Human)
  • Alternate Names:
    CD301 antigen; CD301; CD301C-type lectin domain family 10 member A; CLEC10A; CLECSF13; CLECSF14; CLECSF14macrophage C-type lectin; C-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamilymember 14 (macrophage-derived); C-type lectin domain family 10, member A; C-type lectin superfamily member 14; HML2; HMLC-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamilymember 13 (macrophage-derived); macrophage lectin 2 (calcium dependent); Macrophage lectin 2; MGL
Related Research Areas
Isotype Controls
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Donkey Anti-Goat IgG NorthernLights™ NL637-conjugated Antibody

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Chicken Anti-Goat IgG Biotinylated Antibody

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Rabbit Anti-Goat IgG Biotinylated Antibody

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Rabbit Anti-Goat IgG (H+L) Affinity Purified PAb, X Absorbed

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Donkey Anti-Goat IgG (H+L) PerCP-conjugated Antibody

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