Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Western Blot, Intracellular Staining by Flow Cytometry, CyTOF-ready

Cited:

Immunohistochemistry, Western Blot, Immunocytochemistry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Cyclin D1/D2 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Cyclin D1
Met1-Ile295
Accession # P24385

Specificity

Detects human and mouse Cyclin D1/D2 in Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human/Mouse Cyclin D1/D2 Antibody

Detection of Human/Mouse Cyclin D1/D2 antibody by Western Blot.

Detection of Human/Mouse Cyclin D1/D2 by Western Blot.

Western blot shows lysates of Neuro-2A mouse neuroblastoma cell line, U2OS human osteosarcoma cell line, and MCF-7 human breast cancer cell line. PVDF membrane was probed with 1 µg/mL Goat Anti-Human/Mouse Cyclin D1/D2 Cross-reactive Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4196) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). For additional reference, recombinant human Cyclin D1, D2, and D3 (5 ng/lane) were included. A specific band for Cyclin D1 was detected at approximately 33 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Cyclin D1/D2 antibody in MCF-7 Human Cell Line antibody by Flow Cytometry.

Detection of Cyclin D1/D2 in MCF‑7 Human Cell Line by Flow Cytometry.

MCF-7 human breast cancer cell line was stained with Goat Anti-Human/Mouse Cross-reactive Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4196, filled histogram) or isotype control AA175antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107). To facilitate intracellular staining, cells were fixed with paraformaldeyde and permeabilized with saponin.

Detection of Cyclin D1/D2 by Western Blot

Detection of Cyclin D1/D2 by Western Blot

Aspirin modulated the JAK/p-STAT3 signaling pathway in atypical hyperplastic intestinal mucosal cells of UC mice (n = 4 for each group). (A) Western blotting images for JAK/p-STAT3 signaling pathway-associated molecules expression. (B) Statistical analysis and comparison for p-STAT3 expression. (C) Statistical analysis and comparison for STAT3 expression. (D) Statistical analysis and comparison for cyclin D1 expression. (E) Statistical analysis and comparison for SOCS3 expression. *P <.05 versus control group. #P <.05 versus UC model group. JAK, Janus kinase; UC, ulcerative colitis; p-STAT3, phosphorylated-STAT3; STAT3, signal transducer and activator of transcription 3; SOCS3, suppressor of cytokine signaling 3. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35946886), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Cyclin D1/D2 by Western Blot

Detection of Cyclin D1/D2 by Western Blot

Aspirin modulated the JAK/p-STAT3 signaling pathway in atypical hyperplastic intestinal mucosal cells of UC mice (n = 4 for each group). (A) Western blotting images for JAK/p-STAT3 signaling pathway-associated molecules expression. (B) Statistical analysis and comparison for p-STAT3 expression. (C) Statistical analysis and comparison for STAT3 expression. (D) Statistical analysis and comparison for cyclin D1 expression. (E) Statistical analysis and comparison for SOCS3 expression. *P <.05 versus control group. #P <.05 versus UC model group. JAK, Janus kinase; UC, ulcerative colitis; p-STAT3, phosphorylated-STAT3; STAT3, signal transducer and activator of transcription 3; SOCS3, suppressor of cytokine signaling 3. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35946886), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Human Cyclin D1/D2 by Western Blot

Detection of Human Cyclin D1/D2 by Western Blot

Cyclin D1 mRNA and protein expression in iAAT-MSCs and iMSCs. (A). Cyclin D1 mRNA expression was higher in iAAT-MSCs compared to iMSCs; data are fold changes compared to GAPDH expression in each sample. (B) Expression of Cyclin D1 and phosphorylated (p)-Cyclin D1 and beta -actin in iMSCs and iAAT-MSC. Lanes 1–3 are iMSCs in triplicate and 4–6 are iAAT-MSCs in triplicate. (C,D). Quantification/relative expression of CyclinD1/ beta -actin and p-Cyclin D1/ beta -actin, p-Cyclin D1/total Cyclin D1 at the protein level (C) and ratios (D) in iAAT-MSCs compared to iMSCs. (E) Representative expression of Cyclin D1 stained from cell culture of iMSCs and iAAT-MSCs. Red signals identify Cyclin D and blue staining structures are nuclei. Scale bar = 40 μm. * p < 0.05, Student’s t-test. NS: not significant. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/38396691), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human/Mouse Cyclin D1/D2 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Intracellular Staining by Flow Cytometry

2.5 µg/106 cells
Sample:

MCF‑7 human breast cancer cell line fixed with paraformaldeyde and permeabilized with saponin.

Western Blot

1 µg/mL
Sample: Neuro-2A mouse neuroblastoma cell line, U2OS human osteosarcoma cell line, and MCF-7 human breast cancer cell line

Flow Cytometry Panel Builder

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Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Cyclin D1/D2

The D-type cyclins (cyclins D1, D2, and D3) and their associated kinases, Cdk4 and Cdk6, play an important role in the progression from G0/G1 to S phase in the mammalian cell cycle. Cyclin D complexes containing Cdk4 or Cdk6 phosphorylate the retinoblastoma protein (pRb). pRb phosphorylation leads to the release of E2F transcription factors, which activate the expression of S-phase genes and thereby induce cell cycle progression. Cyclin D1, independent of Cdk4 activity, functions as a transcriptional modulator by regulating the activity of several transcription factors, such as estrogen receptor, Myb, and STAT3. Cyclin D1 has also been linked to the development and progression of several cancers including breast, bladder, esophagus, and lung. Overexpression of Cyclin D2 has been reported in gastric carcinoma, ovarian granulose cell tumors, and hematopoietic cell cancers, while in breast cancers, cyclin D2 expression is undetectable in 80% of the tumors.

Alternate Names

BCL1;CCND1;D11S287E;G1/S-specific cyclin-D1;PRAD1;U21B31

Entrez Gene IDs

595 (Human); 12443 (Mouse); 58919 (Rat)

Gene Symbol

CCND1

UniProt

P24385

Additional Cyclin D1/D2 Products

Product Documents for Human/Mouse Cyclin D1/D2 Antibody

Certificate of Analysis

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Product Specific Notices for Human/Mouse Cyclin D1/D2 Antibody

For research use only

Related Research Areas

Cell Cycle

Citations for Human/Mouse Cyclin D1/D2 Antibody

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