|Detection of Human/Mouse EBF‑1 by Western Blot. Western blot shows lysates of Raji human Burkitt's lymphoma cell line, Daudi human Burkitt's lymphoma cell line, and CH‑1 mouse B cell lymphoma cell line, and L1.2 mouse pro‑B cell line. PVDF membrane was probed with 2 µg/mL of Human/Mouse EBF‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5165) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for EBF‑1 at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.|
EBF-1 (Early B cell Factor 1; also OLF1 and COE1) is a 65-70 kDa member of the COE family of transcription factors. Although expressed in adipocytes and neurons, it is best studied in B cells where IL-7 acts to promote EBF-1 in pre-proB cells, leading to proB stage development. Mouse EBF-1 is 591 amino acids (aa) in length. It contains one DNA-binding region with an embedded zinc-finger motif (aa 51‑235), a dimerization segment between aa 370‑430, and a Pro/Ser-rich transactivation domain (aa 462‑550). EBF-1 either homodimerizes, or heterodimerizes with EBF-2 and -3. There is an alternate start site at Met134, and an isoform that shows a one aa substitution for aa 252‑259. Over aa 416‑520, mouse EBF-1 shows absolute aa identity to the equivalent sequence in rat and human EBF-1.
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