Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Western Blot, Simple Western

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Glutaredoxin 1/GLRX1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human GLRX1
Met1-Gln106
Accession # P35754

Specificity

Detects human and mouse GLRX1 in Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human/Mouse Glutaredoxin 1/GLRX1 Antibody

Detection of Human/Mouse Glutaredoxin 1 antibody by Western Blot.

Detection of Human/Mouse Glutaredoxin 1 by Western Blot.

Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, Jurkat human acute T cell leukemia cell line, NIH-3T3 mouse embryonic fibroblast cell line, and C2C12 mouse myoblast cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse Glutaredoxin 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3399) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Glutaredoxin 1 at approximately 12 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Detection of Human Glutaredoxin 1/GLRX1 antibody by Simple WesternTM.

Detection of Human Glutaredoxin 1/GLRX1 by Simple WesternTM.

Simple Western lane view shows lysates of HepG2 human hepatocellular carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Glutaredoxin 1/GLRX1 at approximately 10 kDa (as indicated) using 5 µg/mL of Goat Anti-Human/Mouse Glutaredoxin 1/GLRX1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3399) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications for Human/Mouse Glutaredoxin 1/GLRX1 Antibody

Application
Recommended Usage

Simple Western

5 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line

Western Blot

0.5 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line, Jurkat human acute T cell leukemia cell line, NIH-3T3 mouse embryonic fibroblast cell line, and C2C12 mouse myoblast cell line

Reviewed Applications

Read 4 reviews rated 4.3 using AF3399 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Glutaredoxin 1/GLRX1

Glutaredoxins or thioltransferases are a universally distributed family of small molecular weight proteins with thiol-disulfide exchange activity.Glutaredoxin 1(GLRX1/GRX) is a 12 kDa protein with glutathione-disulfide oxidoreductase activity. Glutaredoxins utilize the reducing power of glutathione to catalyze disulfide reductions in the presence of NADPH and glutathione reductase (the glutaredoxin system). The amino acid sequence of Glutaredoxin 1 in humans is highly homologous with that in other mammals (81-89%), and contains the conserved active sequence motif -Cys-Pro-Try-Cys-. Glutaredoxin 1 also catalyzes the formation of mixed disulfides (glutathionylation), which is an important redox regulatory mechanism, particularly in mammalian cells under oxidative stress conditions, to sense cellular redox potential.

Alternate Names

GLRX, Thioltransferase

Entrez Gene IDs

2745 (Human); 93692 (Mouse); 64045 (Rat)

Gene Symbol

GLRX

UniProt

P35754

Additional Glutaredoxin 1/GLRX1 Products

Product Documents for Human/Mouse Glutaredoxin 1/GLRX1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse Glutaredoxin 1/GLRX1 Antibody

For research use only

Related Research Areas

Oxidative Stress

Citations for Human/Mouse Glutaredoxin 1/GLRX1 Antibody

Customer Reviews for Human/Mouse Glutaredoxin 1/GLRX1 Antibody (4)

4.3 out of 5
4 Customer Ratings
5 Stars
50%
4 Stars
25%
3 Stars
25%
2 Stars
0%
1 Stars
0%

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Customer Images

Showing  1 - 4 of 4 reviews Showing All
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  • Human/Mouse Glutaredoxin 1/GLRX1 Antibody
    Name: Anonymous
    Application: Microarrays
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 01/14/2021
    Human/Mouse Glutaredoxin 1/GLRX1 Antibody AF3399
  • Human/Mouse Glutaredoxin 1/GLRX1 Antibody
    Name: Anonymous
    Application: Microarrays
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 11/07/2018
  • Human/Mouse Glutaredoxin 1/GLRX1 Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Plasma and Serum
    Species: Human
    Verified Customer | Posted 11/07/2018
  • Human/Mouse Glutaredoxin 1/GLRX1 Antibody
    Name: Carly Garrison
    Application: Microarray
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 10/09/2018

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