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Human/Mouse HMGB3 Antibody

Catalog #: MAB55071 Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB55071
MAB55071-SP

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Detection of Human HMGB3 by Western Blot.
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Human/Mouse HMGB3 Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human and mouse HMGB3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human HMG-1, A1A, A1B, or A2 is observed.
Source
Monoclonal Mouse IgG1 Clone # 546519
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human HMGB3
Met1-Glu180
Accession # O15347
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human HMGB3 antibody by Western Blot. View Larger

Detection of Human HMGB3 by Western Blot. Western blot shows lysates of human placenta tissue. PVDF Membrane was probed with 2 µg/mL of Human/Mouse HMGB3 Monoclonal Antibody (Catalog # MAB55071) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for HMGB3 at approximately 23 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry HMGB3 antibody in Detroit 551 Human Cell Line by Immunocytochemistry (ICC). View Larger

HMGB3 in Detroit 551 Human Cell Line. HMGB3 was detected in immersion fixed Detroit 551 human skin fibroblast cell line using Human/Mouse HMGB3 Monoclonal Antibody (Catalog # MAB55071) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: HMGB3

High mobility group protein B3 (HMGB3; also HMG-4 and HMG-2a) is a 23 kDa nuclear protein and member of the HMGB family.  Human HMGB3 is synthesized as a 200 amino acid (aa) precursor, which is demethylated to produce the 199 aa mature chain.  The protein contains two HMG box DNA-binding domains (aa 9‑79 and 93‑161) and an acidic Asp/Glu-rich region (aa 181‑200).  Human HMGB3 shares 98% aa sequence identity with mouse and bovine HMGB3.  HMGB3 is expressed predominantly in the placenta.  It binds preferentially single-stranded DNA and unwinds double stranded DNA.

Long Name
High Mobility Group Box 3
Entrez Gene IDs
3149 (Human); 15354 (Mouse); 305373 (Rat)
Alternate Names
High mobility group protein 2a; High mobility group protein 4; high mobility group protein B3; high-mobility group (nonhistone chromosomal) protein 4; high-mobility group box 3; HMG2A; HMG-2a; HMG2AHMG-4; HMG4; HMG4MGC90319; HMGB3; non-histone chromosomal protein

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