High mobility group protein B3 (HMGB3; also HMG-4 and HMG-2a) is a 23 kDa nuclear protein and member of the HMGB family. Human HMGB3 is synthesized as a 200 amino acid (aa) precursor, which is demethylated to produce the 199 aa mature chain. The protein contains two HMG box DNA-binding domains (aa 9‑79 and 93‑161) and an acidic Asp/Glu-rich region (aa 181‑200). Human HMGB3 shares 98% aa sequence identity with mouse and bovine HMGB3. HMGB3 is expressed predominantly in the placenta. It binds preferentially single-stranded DNA and unwinds double stranded DNA.
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Human
Applications
Validated:
Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Loading...
Product Specifications
Immunogen
E. coli-derived recombinant human HMGB3
Met1-Val180
Accession # O15347
Met1-Val180
Accession # O15347
Specificity
Detects endogenous human/mouse HMGB3 in Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human/Mouse HMGB3 Antibody
Detection of Human HMGB3 by Western Blot.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, 293T human embryonic kidney cell line, and Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse HMGB3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5507) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for HMGB3 at approximately 29 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of HMGB3 by Western Blot
In vitro functional validation of the HMGB3. (A). The relative expression of HMGB3 in GEPIA. (B). Knockdown efficiency of HMGB3 by two small interferon RNA transfection. (C). Proliferative effect of HMGB3 on Ishikawa evaluated by Cell Counting Kit‐8 tests. (D). Effects of HMGB3 on the invasion of Ishikawa cells evaluated by Transwell assays. (E). Statistical analysis of the Transwell invasion. (F) Effects of MMP12 on the migration of Ishikawa cells evaluated by gap closure assays. (G) Statistical analysis of the gap closure. *p < 0.05, **p < 0.01. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35778922), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of HMGB3 by Western Blot
In vitro functional validation of the HMGB3. (A). The relative expression of HMGB3 in GEPIA. (B). Knockdown efficiency of HMGB3 by two small interferon RNA transfection. (C). Proliferative effect of HMGB3 on Ishikawa evaluated by Cell Counting Kit‐8 tests. (D). Effects of HMGB3 on the invasion of Ishikawa cells evaluated by Transwell assays. (E). Statistical analysis of the Transwell invasion. (F) Effects of MMP12 on the migration of Ishikawa cells evaluated by gap closure assays. (G) Statistical analysis of the gap closure. *p < 0.05, **p < 0.01. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35778922), licensed under a CC-BY license. Not internally tested by R&D Systems.Applications for Human/Mouse HMGB3 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line, 293T human embryonic kidney cell line, and Jurkat human acute T cell leukemia cell line
Sample: HeLa human cervical epithelial carcinoma cell line, 293T human embryonic kidney cell line, and Jurkat human acute T cell leukemia cell line
Reviewed Applications
Read 1 review rated 4 using AF5507 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Loading...
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: HMGB3
Long Name
High Mobility Group Box 3
Alternate Names
HMG2A, HMG4
Gene Symbol
HMGB3
UniProt
Additional HMGB3 Products
Product Documents for Human/Mouse HMGB3 Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human/Mouse HMGB3 Antibody
For research use only
Related Research Areas
Citations for Human/Mouse HMGB3 Antibody
Powered by Bioz
Customer Reviews for Human/Mouse HMGB3 Antibody (1)
4 out of 5
1 Customer Rating
Have you used Human/Mouse HMGB3 Antibody?
Submit a review and receive an Amazon gift card!
$25/€18/£15/$25CAN/¥2500 Yen for a review with an image
$10/€7/£6/$10CAN/¥1110 Yen for a review without an image
Submit a review
Showing
1
-
1 of
1 review
Showing All
Filter By:
-
Application: Western BlotSample Tested: See PMID 22912879Species: MouseVerified Customer | Posted 01/08/2015
There are no reviews that match your criteria.
Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
Loading...