Human/Mouse HMGB3 Antibody

Catalog # Availability Size / Price Qty
AF5507
AF5507-SP
Detection of Human HMGB3 by Western Blot.
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Product Details
Citations (3)
FAQs
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Human/Mouse HMGB3 Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects endogenous human/mouse HMGB3 in Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human HMGB3
Met1-Val180
Accession # O15347
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human HMGB3 antibody by Western Blot. View Larger

Detection of Human HMGB3 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, 293T human embryonic kidney cell line, and Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse HMGB3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5507) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for HMGB3 at approximately 29 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: HMGB3

High mobility group protein B3 (HMGB3; also HMG-4 and HMG-2a) is a 23 kDa nuclear protein and member of the HMGB family. Human HMGB3 is synthesized as a 200 amino acid (aa) precursor, which is demethylated to produce the 199 aa mature chain. The protein contains two HMG box DNA-binding domains (aa 9‑79 and 93‑161) and an acidic Asp/Glu-rich region (aa 181‑200). Human HMGB3 shares 98% aa sequence identity with mouse and bovine HMGB3. HMGB3 is expressed predominantly in the placenta. It binds preferentially single-stranded DNA and unwinds double stranded DNA.

Long Name
High Mobility Group Box 3
Entrez Gene IDs
3149 (Human); 15354 (Mouse); 305373 (Rat)
Alternate Names
High mobility group protein 2a; High mobility group protein 4; high mobility group protein B3; high-mobility group (nonhistone chromosomal) protein 4; high-mobility group box 3; HMG2A; HMG-2a; HMG2AHMG-4; HMG4; HMG4MGC90319; HMGB3; non-histone chromosomal protein

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Citations for Human/Mouse HMGB3 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Construction and validation of a prognostic model based on 11 lymph node metastasis‐related genes for overall survival in endometrial cancer
    Authors: Hong Wu, Haiqin Feng, Xiaoli Miao, Jiancai Ma, Cairu Liu, Lina Zhang et al.
    Cancer Medicine
  2. High mobility group box 3 promotes cervical cancer proliferation by regulating Wnt/ beta -catenin pathway
    Authors: Shichao Zhuang, Xiaohui Yu, Ming Lu, Yujiao Li, Ning Ding, Yumei Ding
    Journal of Gynecologic Oncology
  3. MicroRNA‑758 inhibits cervical cancer cell proliferation and metastasis by targeting HMGB3 through the WNT/ beta ‑catenin signaling pathway
    Authors: Tao Song, Xinghua Hou, Bing Lin
    Oncology Letters

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