Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Western Blot

Cited:

Western Blot, RNA immunoprecipitation, Chromatin Immunoprecipitation (ChIP)

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all HSF2 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human HSF2
Ser411-Ser536
Accession # Q03933

Specificity

Detects endogenous human and mouse HSF2 in Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human/Mouse HSF2 Antibody

Detection of Human/Mouse HSF2 antibody by Western Blot.

Detection of Human/Mouse HSF2 by Western Blot.

Western blot shows lysates of NIH-3T3 mouse embryonic fibroblast cell line, human HeLa human cervical epithelial carcinoma cell line, and 293T human embryonic kidney cell line. PVDF membrane was probed with 2 µg/mL of Human/Mouse HSF2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5227) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for HSF2 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

Applications for Human/Mouse HSF2 Antibody

Application
Recommended Usage

Western Blot

2 µg/mL
Sample: NIH-3T3 mouse embryonic fibroblast cell line, human HeLa human cervical epithelial carcinoma cell line, and 293T human embryonic kidney cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: HSF2

HSF2 (Heat shock transcription factor 2; also HSTF2) is a 60 kDa member of the HSF family of proteins. It is widely expressed and serves as a transcriptional activator of HSPs. In quiescent cells, HSF2 is a cytoplasmic homodimer; upon exposure to stress or differentiation, it homo- or hetero-trimerizes with HSF-1, and translocates to the nucleus where it impacts gene transcription. Human HSF2 is 536 amino acids (aa) in length and contains a DNA binding region (aa 7‑107), two NLSs (aa 108‑122 and 195‑210) and an HR (hydrophobic repeat) domain that mediates trimerization (aa 119‑192). There are three potential splice variants. One shows a Leu substitution for aa 260‑536, a second shows an IlePhe substitution for aa 229‑536, and a third shows a deletion of aa 393‑410. Over aa 411‑536, human HSF2 is 92% aa identical to mouse HSF2.

Long Name

Heat Shock Transcription Factor 2

Alternate Names

HSTF2

Entrez Gene IDs

3298 (Human)

Gene Symbol

HSF2

UniProt

Q03933

Additional HSF2 Products

Product Documents for Human/Mouse HSF2 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse HSF2 Antibody

For research use only

Citations for Human/Mouse HSF2 Antibody

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