HSF2 Antibody

Detection of Human/Mouse HSF2 by Western Blot.

Western blot shows lysates of NIH-3T3 mouse embryonic fibroblast cell line, human HeLa human cervical epithelial carcinoma cell line, and 293T human embryonic kidney cell line. PVDF membrane was probed with 2 µg/mL of Human/Mouse HSF2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5227) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for HSF2 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

Detection of HSF2 by Western Blot

Heat shock initiates the formation of a transcriptional regulatory network that may be stronger in HSF1-deficient MCF7 cells. (A) Networks of upstream regulators of HS-stimulated (HS up) or repressed (HS down) genes (identified by RNA-seq analyses in HSF1prof and HSF1def cells) predicted using the ChEA3_ReMap (FDR < 0.05, top 13 factors are shown). (B) HSF1 peaks in the regulatory regions of ATF3 and JUN and FOS family members identified in ChIP-seq analyses and visualized by the IGV browser in untreated cells (Ctr) and after heat shock (HS). The scale for each sample is displayed in the left corner, length of the region shown—in the right corner. (C) Heat-induced changes in expression of selected genes in HSF1prof and HSF1def cells (extracted from RNA-seq data). ** p < 0.001, * p < 0.05 (significance of differences versus the corresponding control or between cell variants). (D) Western blot analysis of HSF2 levels up to 6 h of recovery from heat shock in HSF1+ and HSF1− cells. ACTB was used as loading control, and HSF1 S326 phosphorylation was analyzed to monitor HSF1 activation after heat shock. (E) HSF1 (upper panel) and HSF2 (bottom panel) binding (by ChIP-qPCR; % of input) in selected sequences in untreated (Ctr) and after HS treatment (43 °C, 15 min) HSF1+ and HSF1− cells. Two analyzed binding sites in ATF3 are depicted in (B). ** p < 0.001, * p < 0.05. Image collected and cropped by CiteAb from the following open publication (https://www.mdpi.com/2073-4409/11/16/2510), licensed under a CC-BY license. Not internally tested by R&D Systems.