HSF2 Antibody

R&D Systems | Catalog # AF5227

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Western Blot

Cited:

Western Blot, RNA immunoprecipitation, Chromatin Immunoprecipitation (ChIP)

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all HSF2 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human HSF2
Ser411-Ser536
Accession # Q03933

Specificity

Detects endogenous human and mouse HSF2 in Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for HSF2 Antibody

Detection of Human/Mouse HSF2 antibody by Western Blot.

Detection of Human/Mouse HSF2 by Western Blot.

Western blot shows lysates of NIH-3T3 mouse embryonic fibroblast cell line, human HeLa human cervical epithelial carcinoma cell line, and 293T human embryonic kidney cell line. PVDF membrane was probed with 2 µg/mL of Human/Mouse HSF2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5227) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for HSF2 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

Applications for HSF2 Antibody

Application
Recommended Usage

Western Blot

2 µg/mL
Sample: NIH-3T3 mouse embryonic fibroblast cell line, human HeLa human cervical epithelial carcinoma cell line, and 293T human embryonic kidney cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: HSF2

HSF2 (Heat shock transcription factor 2; also HSTF2) is a 60 kDa member of the HSF family of proteins. It is widely expressed and serves as a transcriptional activator of HSPs. In quiescent cells, HSF2 is a cytoplasmic homodimer; upon exposure to stress or differentiation, it homo- or hetero-trimerizes with HSF-1, and translocates to the nucleus where it impacts gene transcription. Human HSF2 is 536 amino acids (aa) in length and contains a DNA binding region (aa 7‑107), two NLSs (aa 108‑122 and 195‑210) and an HR (hydrophobic repeat) domain that mediates trimerization (aa 119‑192). There are three potential splice variants. One shows a Leu substitution for aa 260‑536, a second shows an IlePhe substitution for aa 229‑536, and a third shows a deletion of aa 393‑410. Over aa 411‑536, human HSF2 is 92% aa identical to mouse HSF2.

Long Name

Heat Shock Transcription Factor 2

Alternate Names

HSTF2

Entrez Gene IDs

3298 (Human)

Gene Symbol

HSF2

UniProt

Q03933

Additional HSF2 Products

Product Documents for HSF2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for HSF2 Antibody

For research use only

Customer Reviews for HSF2 Antibody

There are currently no reviews for this product. Be the first to review HSF2 Antibody and earn rewards!

Have you used HSF2 Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies