Human/Mouse Kilon/NEGR1 Antibody

Catalog # Availability Size / Price Qty
Detection of Human and Mouse Kilon/NEGR1 by Western Blot.
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Product Details
Citations (1)
Supplemental Products

Human/Mouse Kilon/NEGR1 Antibody Summary

Species Reactivity
Human, Mouse
Detects Kilon/NEGR1 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) LAMP and rhNCAM-L1 is observed.
Polyclonal Goat IgG
Antigen Affinity-purified
Mouse myeloma cell line NS0-derived recombinant human Kilon/NEGR1
Accession # Q7Z3B1
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human and Mouse Kilon/NEGR1 antibody by Western Blot. View Larger

Detection of Human and Mouse Kilon/NEGR1 by Western Blot. Western blot shows lysates of human brain cortex and mouse brain tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse Kilon/NEGR1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5394) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Kilon/NEGR1 at approximately 46 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Reconstitution Calculator

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Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Kilon/NEGR1

Kindred of IgLON (Kilon; also neuronal growth regulator 1 (NEGR1) and neurotractin) is a 46 kDa member of the IgLON family of molecules. This cell adhesion family includes the proteins LAMP, OBCAM, neurotrimin, CEPU-1, AvGP50, and GP55 (1). Human Kilon is synthesized as a 354 amino acid (aa) precursor that contains a 37 aa signal sequence, a 287 aa mature chain, and a 30 aa propeptide. The mature chain consists of three C2 Ig-like domains, six potential sites for N-linked glycosylation, and a GPI anchor. In addition, there are three sets of cysteines that have the potential to form intradomain disulfide linkages in each of the mature chain's Ig-like domains (1). Human Kilon shares 97% aa sequence identity with mouse and rat Kilon. Expression of Kilon is restricted to the brain, specifically in the cerebrum, brain stem, and hippocampus, with much less expression in the cerebellum (1). In the rat, it was shown that Kilon is already expressed in the E16 stage, and its level gradually increases during development (1). In the cerebral cortex, numerous puncta of Kilon immunoreactivity were visible in all regions, and most were densely distributed in large neurons of layer V (1). These neurons were identified as pyramidal neurons because of their soma location in layer V, large soma size, and extension of their apical dendrite to layer I (1). Kilon may be involved in cell-adhesion and may function as a trans-neural growth-promoting factor in regenerative axon sprouting in the mammalian brain (1, 2).

  1. Funatsu, N. et al. (1999) J. Biol. Chem. 274:8224.
  2. Marg, A. et al. (1999) J. Cell Biol. 145:865.
Long Name
A Kindred of IgLON /Neuronal Growth Regulator 1
Entrez Gene IDs
257194 (Human); 320840 (Mouse); 59318 (Rat)
Alternate Names
a kindred of IgLON; IgLON family member 4; IGLON4DMML2433; Kilon; KILONMGC46680; NEGR1; neuronal growth regulator 1; Neurotractin; Ntra

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Citation for Human/Mouse Kilon/NEGR1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Synapse type-specific proteomic dissection identifies IgSF8 as a hippocampal CA3 microcircuit organizer
    Authors: N Apóstolo, SN Smukowski, J Vanderlind, G Condomitti, V Rybakin, J Ten Bos, L Trobiani, S Portegies, KM Vennekens, NV Gounko, D Comoletti, KD Wierda, JN Savas, J de Wit
    Nat Commun, 2020;11(1):5171.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot


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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Goat IgG (H+L) Antibody


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