Human/Mouse MEF2C Antibody

Catalog # Availability Size / Price Qty
AF6786
AF6786-SP
Detection of Human MEF2C by Western Blot.
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Human/Mouse MEF2C Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human MEF2C in direct ELISAs and Western blots.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human MEF2C
Ala135-Lys239
Accession # Q06413
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.2 µg/mL
See below
Immunohistochemistry
1-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human MEF2C by Western Blot. View Larger

Detection of Human MEF2C by Western Blot. Western blot shows lysates of K562 human chronic myelogenous leukemia cell line. Gels were loaded with 20 µg of cytoplasmic (Cyto) and 10 µg of nuclear (Nuc) extracts. PVDF Membrane was probed with 0.2 µg/mL of Sheep Anti-Human/Mouse MEF2C Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6786) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for MEF2C at approximately 52-60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry MEF2C  in Human Brain. View Larger

MEF2C in Human Brain. MEF2C was detected in immersion fixed paraffin-embedded sections of human brain (hippocampus) using Sheep Anti-Human/
Mouse MEF2C Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6786) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal nuclei. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Immunohistochemistry MEF2C  in Mouse Brain. View Larger

MEF2C in Mouse Brain. MEF2C was detected in perfusion fixed frozen sections of mouse brain (cortex) using Sheep Anti-Human/Mouse MEF2C Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6786) at 1 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal nuclei. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MEF2C

MEF2C (Myocyte enhancer factor-2C) is a transcriptional activator that is a member of the MEF2 subfamily, MADS (MCM1, Agamous, Deficiens, Serum response factor) gene family of proteins. Although its predicted MW is 51 kDa, it runs anomalously at 57 kDa in SDS-PAGE. It is expressed in B cells, plus postmitotic neurons and skeletal muscle cells that are undergoing differentiation. Human MEF2C is 473 amino acids (aa) in length. It contains a MADS box for dimerization (aa 3-57), a DNA binding domain (aa 58-86), a beta-domain that enhances transcription (aa 271-278), a gamma-domain that, when phosphorylated, promotes transcriptional repression (aa 390-399), and seven acetylation sites plus multiple Ser/Thr phosphorylation sites. Sumoylation occurs on Lys391, and proteolytic cleavage of MEF2C occurs in neurons between Asp432Gly433. Multiple splice forms exist. Either individually or in combination, there may be a deletion of aa 271-278, 368-399 or 87-134, a 46 aa substitution for aa 107-134 and 87-134, and an alternative start site 35 aa upstream of the standard site. Over aa 135-239, human MEF2C shares 96% aa identity with mouse MEF2C.

Long Name
Myocyte-specific Enhancer Factor 2C
Entrez Gene IDs
4208 (Human); 17260 (Mouse); 499497 (Rat)
Alternate Names
DEL5q14.3; MEF2C; myocyte enhancer factor 2C; myocyte-specific enhancer factor 2C; polypeptide C

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Cell and Tissue Staining Kits

Immunohistochemistry Reagents

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Reconstitution Buffers

Secondary Antibodies

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