Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all NCOA3 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human NCOA3
Met673-Val856
Accession # Q9Y6Q9

Specificity

Detects endogenous human and mouse NCOA3 in Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human/Mouse NCOA3 Antibody

Detection of Human NCOA3 antibody by Western Blot.

Detection of Human NCOA3 by Western Blot.

Western blot shows nuclear extracts of Jurkat human acute T cell leukemia cell line, HeLa human cervical epithelial carcinoma cell line, Daudi human Burkitt's lymphoma cell line, and MCF-7 human breast cancer cell line. PVDF membrane was probed with 0.1 µg/mL of Human/Mouse NCOA3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5076) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for NCOA3 at approximately 170 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human/Mouse NCOA3 Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Jurkat human acute T cell leukemia cell line, HeLa human cervical epithelial carcinoma cell line, Daudi human Burkitt's lymphoma cell line, and MCF-7 human breast cancer cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: NCOA3

Nuclear Receptor Coactivator 3 (NCOA3) functions in the multisubunit coactivator complex. Multiple nuclear hormone receptors are able to bind to NCOA3 in a hormone dependent manor. NCOA3 contains intrinsic histone acetyltransferase activity, but is also able to recruit other histone aceytltransferases (CBP, PCAF) and methyltransferases (PRMT1, CARM1) to active promoters. NCOA3 null mice exhibit hematological, adiopogenesis, and endotoxic impairments. mRNA of NCOA3 is often amplified in several cancers including breast and ovarian.

Long Name

Nuclear Receptor Co-activator 3

Alternate Names

ACTR, AIB1, KAT13B, pCIP, RAC3, SRC-1, SRC-3, TRAM1

Entrez Gene IDs

8202 (Human); 17979 (Mouse); 84584 (Rat)

Gene Symbol

NCOA3

UniProt

Q9Y6Q9

Additional NCOA3 Products

Product Documents for Human/Mouse NCOA3 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse NCOA3 Antibody

For research use only

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