Human/Mouse NCOA3 Antibody

Catalog # Availability Size / Price Qty
Detection of Human NCOA3 by Western Blot.
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Product Details
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Human/Mouse NCOA3 Antibody Summary

Species Reactivity
Human, Mouse
Detects endogenous human and mouse NCOA3 in Western blots.
Polyclonal Goat IgG
Antigen Affinity-purified
E. coli-derived recombinant human NCOA3
Accession # Q9Y6Q9
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
0.1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human NCOA3 antibody by Western Blot. View Larger

Detection of Human NCOA3 by Western Blot. Western blot shows nuclear extracts of Jurkat human acute T cell leukemia cell line, HeLa human cervical epithelial carcinoma cell line, Daudi human Burkitt's lymphoma cell line, and MCF-7 human breast cancer cell line. PVDF membrane was probed with 0.1 µg/mL of Human/Mouse NCOA3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5076) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for NCOA3 at approximately 170 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NCOA3

Nuclear Receptor Coactivator 3 (NCOA3) functions in the multisubunit coactivator complex. Multiple nuclear hormone receptors are able to bind to NCOA3 in a hormone dependent manor. NCOA3 contains intrinsic histone acetyltransferase activity, but is also able to recruit other histone aceytltransferases (CBP, PCAF) and methyltransferases (PRMT1, CARM1) to active promoters. NCOA3 null mice exhibit hematological, adiopogenesis, and endotoxic impairments. mRNA of NCOA3 is often amplified in several cancers including breast and ovarian.

Long Name
Nuclear Receptor Co-activator 3
Entrez Gene IDs
8202 (Human); 17979 (Mouse); 84584 (Rat)
Alternate Names
ACTR; ACTRCBP-interacting protein; AIB1; AIB1AIB-1; Amplified in breast cancer 1 protein; BHLHE42; bHLHe42p/CIP; CAGH16; Class E basic helix-loop-helix protein 42; CTG26; KAT13B; MGC141848; NCOA3; NCoA-3; nuclear receptor coactivator 3; pCIP ; pCIP; RAC3; RAC-3; RAC3pCIP; Receptor-associated coactivator 3; SRC-1; SRC3; SRC-3; SRC-3EC; Steroid receptor coactivator protein 3; Thyroid hormone receptor activator molecule 1; TNRC16; TRAM1; TRAM-1TNRC14

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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