|Detection of Human NCOA3 by Western Blot. Western blot shows nuclear extracts of Jurkat human acute T cell leukemia cell line, HeLa human cervical epithelial carcinoma cell line, Daudi human Burkitt's lymphoma cell line, and MCF‑7 human breast cancer cell line. PVDF membrane was probed with 0.1 µg/mL of Human/Mouse NCOA3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5076) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for NCOA3 at approximately 170 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.|
Nuclear Receptor Coactivator 3 (NCOA3) functions in the multisubunit coactivator complex. Multiple nuclear hormone receptors are able to bind to NCOA3 in a hormone dependent manor. NCOA3 contains intrinsic histone acetyltransferase activity, but is also able to recruit other histone aceytltransferases (CBP, PCAF) and methyltransferases (PRMT1, CARM1) to active promoters. NCOA3 null mice exhibit hematological, adiopogenesis, and endotoxic impairments. mRNA of NCOA3 is often amplified in several cancers including breast and ovarian.
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