Human/Mouse NETO2 Antibody

  
  • Species Reactivity
    Human, Mouse
  • Specificity
    Detects human and mouse NETO2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 10% cross-reactivity with recombinant human NETO1 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human NETO2 isoform 1
    Ile23-Lys345
    Accession # Q8NC67
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL

    Recombinant Human NETO2
    Recombinant Mouse NETO2

  • Immunoprecipitation
    1-2 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunoprecipitation
Immunoprecipitation of Mouse NETO2. NETO2 was immunoprecipitated from lysates (500 µg) of mouse brain tissue following incubation with 1‑2 µg/mL Goat Anti-Human/Mouse NETO2 Antigen Affinity-purified Polyclonal Antibody for 8 hours at 2‑8 °C. NETO2-antibody complexes were absorbed using Protein G (Sigma). Immunoprecipitated NETO2 was detected by Western blot using 1 µg/mL Goat Anti-Human/Mouse NETO2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3859). View our recommended buffer recipes for immunoprecipitation.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: NETO2
NETO2 is a neurospecific type I transmembrane protein belonging to the NETO family of proteins that share homology with the axon guidance receptors, neuropilin and the developmentally important metalloproteinase Tolloid/BMP-1. NETO proteins have two CUB domains, an LDL-receptor class A domain in their extracellular region and a conserved FXNPXY-like domain in the cytoplasmic region. A splice variant of NETO2 that lacks the CUB domains and most of the LDL class A domain has been reported. It is likely that like NETO1, NETO2 may be a synaptic protein with neurological function. Human and mouse NETO2 share 98% amino acid sequence homology in their extracellular domains.
  • Long Name:
    Neuropilin [NRP] And Tolloid [TLL]-like 2
  • Entrez Gene IDs:
    81831 (Human); 74513 (Mouse); 307757 (Rat)
  • Alternate Names:
    Brain-specific transmembrane protein containing 2 CUB and 1 LDL-receptor classA domains protein 2; BTCL2; FLJ10430; FLJ90456; NEOT2; NEOT2FLJ14724; NETO2; neuropilin (NRP) and tolloid (TLL)-like 2; neuropilin and tolloid-like protein 2
ExactaChIP Chromatin Immunoprecipitation Kits
Description Application Cat# Citations Images  

ExactaChIP Chromatin IP Buffer Panel

ChIP ECP001 2
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Isotype Controls
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Normal Goat IgG Control

Ctrl AB-108-C 191  
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Secondary Antibodies
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Goat IgG HRP-conjugated Antibody

WB, Simple Western HAF109 19  
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Goat IgG HRP-conjugated Antibody

WB HAF017 15  
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Goat IgG (H+L) PE-conjugated Antibody

Flow F0107 4  
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Donkey Anti-Goat IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL001 9
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Donkey Anti-Goat IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL003 5
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Goat IgG (H+L) APC-conjugated Antibody

Flow F0108 6  
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Goat IgG Horseradish Peroxidase-conjugated Antibody

WB HAF019 6  
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Donkey Anti-Goat IgG Antibody

WB AF109 6
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Donkey Anti-Goat IgG Biotinylated Antibody

WB BAF109 3
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Goat IgG VisUCyte HRP Polymer

IHC VC004  
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Goat IgG (H+L) Fluorescein-conjugated Antibody

Flow F0109 2  
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Donkey Anti-Goat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL002
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Chicken Anti-Goat IgG Biotinylated Antibody

WB BAF019
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Rabbit Anti-Goat IgG Biotinylated Antibody

WB BAF017
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Rabbit Anti-Goat IgG (H+L) Affinity Purified PAb, X Absorbed

WB, ELISA R-401-C-ABS
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Donkey Anti-Goat IgG (H+L) PerCP-conjugated Antibody

Flow F0124
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