Immunoprecipitation of Mouse NETO2. NETO2 was immunoprecipitated from lysates (500 µg) of mouse brain tissue following incubation with 1‑2 µg/mL Goat Anti-Human/Mouse NETO2 Antigen Affinity-purified Polyclonal Antibody for 8 hours at 2‑8 °C. NETO2-antibody complexes were absorbed using Protein G (Sigma). Immunoprecipitated NETO2 was detected by Western blot using 1 µg/mL Goat Anti-Human/Mouse NETO2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3859). View our recommended buffer recipes for immunoprecipitation.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
NETO2 is a neurospecific type I transmembrane protein belonging to the
NETO family of proteins that share homology with the axon guidance
receptors, neuropilin and the developmentally important
metalloproteinase Tolloid/BMP-1. NETO proteins have two CUB domains, an
LDL-receptor class A domain in their extracellular region and a
conserved FXNPXY-like domain in the cytoplasmic region. A splice
variant of NETO2 that lacks the CUB domains and most of the LDL class A
domain has been reported. It is likely that like NETO1, NETO2 may be a
synaptic protein with neurological function. Human and mouse NETO2
share 98% amino acid sequence homology in their extracellular domains.
Neuropilin [NRP] And Tolloid [TLL]-like 2
Entrez Gene IDs:
81831 (Human); 74513 (Mouse); 307757 (Rat)
Brain-specific transmembrane protein containing 2 CUB and 1 LDL-receptor classA domains protein 2; BTCL2; FLJ10430; FLJ90456; NEOT2; NEOT2FLJ14724; NETO2; neuropilin (NRP) and tolloid (TLL)-like 2; neuropilin and tolloid-like protein 2
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