Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all NETO2 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human NETO2 isoform 1
Ile23-Lys345
Accession # Q8NC67

Specificity

Detects human and mouse NETO2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 10% cross-reactivity with recombinant human NETO1 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human/Mouse NETO2 Antibody

Immunoprecipitation of Mouse NETO2.

Immunoprecipitation of Mouse NETO2.

NETO2 was immunoprecipitated from lysates (500 µg) of mouse brain tissue following incubation with 1‑2 µg/mL Goat Anti-Human/Mouse NETO2 Antigen Affinity-purified Polyclonal Antibody for 8 hours at 2‑8 °C. NETO2-antibody complexes were absorbed using Protein G (Sigma). Immunoprecipitated NETO2 was detected by Western blot using 1 µg/mL Goat Anti-Human/Mouse NETO2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3859). View our recommended buffer recipes for immunoprecipitation.

Applications for Human/Mouse NETO2 Antibody

Application
Recommended Usage

Immunoprecipitation

1-2 µg/mL
Sample: Mouse brain tissue, see our available Western blot detection antibodies

Western Blot

0.1 µg/mL
Sample:

Recombinant Human NETO2
Recombinant Mouse NETO2

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: NETO2

NETO2 is a neurospecific type I transmembrane protein belonging to the NETO family of proteins that share homology with the axon guidance receptors, neuropilin and the developmentally important metalloproteinase Tolloid/BMP-1. NETO proteins have two CUB domains, an LDL-receptor class A domain in their extracellular region and a conserved FXNPXY-like domain in the cytoplasmic region. A splice variant of NETO2 that lacks the CUB domains and most of the LDL class A domain has been reported. It is likely that like NETO1, NETO2 may be a synaptic protein with neurological function. Human and mouse NETO2 share 98% amino acid sequence homology in their extracellular domains.

Long Name

Neuropilin [NRP] And Tolloid [TLL]-like 2

Alternate Names

BTCL2, NEOT2

Entrez Gene IDs

81831 (Human); 74513 (Mouse); 307757 (Rat)

Gene Symbol

NETO2

UniProt

Q8NC67

Additional NETO2 Products

Product Documents for Human/Mouse NETO2 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse NETO2 Antibody

For research use only

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