Key Product Details

Validated by

Biological Validation

Species Reactivity

Human, Mouse

Applications

Western Blot, Chromatin Immunoprecipitation (ChIP)

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all NFkB1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human NF kappa B1
Pro317-Asp437
Accession # NP_003989

Specificity

Detects endogenous human and mouse NF kappa B1 in Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human/Mouse NFkB1 Antibody

Detection of Human NF?B1 antibody by Western Blot.

Detection of Human NF kappa B1 by Western Blot.

Western blot shows lysates of Raji human Burkitt's lymphoma cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse NF kappa B1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2697) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for NF kappa B1 at approximately 50 and 105 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of NF?B1-regulated Genes antibody by Chromatin Immunoprecipitation.

Detection of NF kappa B1-regulated Genes by Chromatin Immunoprecipitation.

Jurkat human acute T cell leukemia cell line treated with 50 ng/mL PMA and 200 ng/mL calcium ionomycin overnight was fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. NF kappa B1/DNA complexes were immunoprecipitated using 5 µg Goat Anti-Human/Mouse NF kappa B1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2697) or control antibody (Catalog # AB-108-C) for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Goat IgG Secondary Antibody (Catalog # BAF109). Immunocomplexes were captured using 50 µL of MagCellect Streptavidin Ferrofluid (Catalog # MAG999) and DNA was purified using chelating resin solution. Thebcl-2promoter was detected by standard PCR. This application has not been tested in mouse samples.

Applications for Human/Mouse NFkB1 Antibody

Application
Recommended Usage

Chromatin Immunoprecipitation (ChIP)

5 µg/5 x 106 cells
Sample: PMA and calcium ionomycin treated Jurkat human acute T cell leukemia cell line chromatin, bcl-2 promoter detected by standard PCR.

Western Blot

0.5 µg/mL
Sample: Raji human Burkitt's lymphoma cell line and CH-1 mouse B cell lymphoma cell line

Reviewed Applications

Read 1 review rated 5 using AF2697 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: NFkB1

Nuclear Factor kappa B1 (NF kappa B1 or NF kappa B p50) is a member of the rel/NF kappa B family of transcription factors. NF kappa B1 dimerizes with other members of the rel/NF kappa B family to regulate expression of genes that participate in immune, apoptotic, and oncogenic processes.

Long Name

Nuclear Factor Kappa-B, Subunit 1

Alternate Names

NF-kB1

Entrez Gene IDs

4790 (Human); 18033 (Mouse)

Gene Symbol

NFKB1

UniProt

NP_003989

Additional NFkB1 Products

Product Documents for Human/Mouse NFkB1 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse NFkB1 Antibody

For research use only

Citations for Human/Mouse NFkB1 Antibody

Customer Reviews for Human/Mouse NFkB1 Antibody (1)

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  • Human/Mouse NFkB1 Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Cancer cell lysates
    Species: Human
    Verified Customer | Posted 07/16/2020
    Human/Mouse NFkB1 Antibody AF2697

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