Human/Mouse NFkB1 Antibody

Catalog # Availability Size / Price Qty
Detection of Human NF kappa B1 by Western Blot.
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Human/Mouse NFkB1 Antibody Summary

Species Reactivity
Human, Mouse
Detects endogenous human and mouse NF kappa B1 in Western blots.
Polyclonal Goat IgG
Antigen Affinity-purified
E. coli-derived recombinant human NF kappa B1
Accession # NP_003989
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
0.5 µg/mL
See below
Chromatin Immunoprecipitation (ChIP)
5 µg/5 x 106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human NF?B1 antibody by Western Blot. View Larger

Detection of Human NF kappa B1 by Western Blot. Western blot shows lysates of Raji human Burkitt's lymphoma cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse NF kappa B1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2697) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for NF kappa B1 at approximately 50 and 105 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Chromatin Immunoprecipitation (ChIP) Detection of NF?B1-regulated Genes antibody by Chromatin Immunoprecipitation. View Larger

Detection of NF kappa B1-regulated Genes by Chromatin Immunoprecipitation. Jurkat human acute T cell leukemia cell line treated with 50 ng/mL PMA and 200 ng/mL calcium ionomycin overnight was fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. NF kappa B1/DNA complexes were immunoprecipitated using 5 µg Goat Anti-Human/Mouse NF kappa B1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2697) or control antibody (Catalog # AB-108-C) for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Goat IgG Secondary Antibody (Catalog # BAF109). Immunocomplexes were captured using 50 µL of MagCellect Streptavidin Ferrofluid (Catalog # MAG999) and DNA was purified using chelating resin solution. Thebcl-2promoter was detected by standard PCR. This application has not been tested in mouse samples.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NFkB1

Nuclear Factor kappa B1 (NF kappa B1 or NF kappa B p50) is a member of the rel/NF kappa B family of transcription factors. NF kappa B1 dimerizes with other members of the rel/NF kappa B family to regulate expression of genes that participate in immune, apoptotic, and oncogenic processes.

Long Name
Nuclear Factor Kappa-B, Subunit 1
Entrez Gene IDs
4790 (Human); 18033 (Mouse)
Alternate Names
DNA-binding factor KBF1; EBP-1; NF-kappa-B; NFkB1; NF-kB1; NFKB-p105; Nuclear factor NF-kappa-B p105 subunit; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1; p105

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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Human/Mouse NFkB1 Antibody
By Anonymous on 07/16/2020
Application: ELISA Sample Tested: Cancer cell lysates Species: Human