Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Applications

Validated:

Western Blot

Cited:

Immunocytochemistry

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all NPM1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human NPM1
Met9-Leu158
Accession # P06748

Specificity

Detects human and mouse NPM1 in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human/Mouse NPM1 Antibody

Detection of Human and Mouse NPM1 antibody by Western Blot.

Detection of Human and Mouse NPM1 by Western Blot.

Western blot shows lysates of Nalm-6 human Pre-B acute lymphocytic leukemia cell line, Jurkat human acute T cell leukemia cell line and mouse spleen tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse NPM1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5205) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for NPM1 at approximately 37 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Applications for Human/Mouse NPM1 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Nalm‑6 human Pre‑B acute lymphocytic leukemia cell line, Jurkat human acute T cell leukemia cell line and mouse spleen tissue

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: NPM1

NPM1 (Nucleophosmin 1; also nucleolar protein 23) is a 40 kDa member of the nucleoplasmin family of proteins. It is found in the nucleus where it performs multiple functions. NPM1 normally associates with unduplicated centrosomes. After phosphorylation at Thr199, it dissociates from centrosomes, allowing for centrosome duplication. NPM1 also negatively regulates GCN5-dependent histone acetylation during mitosis. Human NPM1 is 294 amino acids (aa) in length. It contains a Met-rich region (aa 1‑9), two acidic regions (aa 120‑132 and 161‑188) and two NLSs (aa 152‑157 and 191‑197). Phosphorylation occurs at Ser4/10/70/254. Splice variants show a deletion of aa 195‑223, a two aa substitution for aa 258‑294 and an 11 aa substitution for aa 288‑294. Over aa 9‑158, human NPM1 is 97% aa identical to mouse NPM1.

Long Name

Nucleophosmin/Nucleolar Phosphoprotein B23

Alternate Names

B23, Nucleophosmin, Numatrin

Entrez Gene IDs

4869 (Human); 434373 (Mouse); 300303 (Rat)

Gene Symbol

NPM1

UniProt

P06748

Additional NPM1 Products

Product Documents for Human/Mouse NPM1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human/Mouse NPM1 Antibody

For research use only

Related Research Areas

Acute Myeloid Leukemia
p53 Pathway

Citations for Human/Mouse NPM1 Antibody

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