|Detection of Human and Mouse NPM1 by Western Blot. Western blot shows lysates of Nalm‑6 human Pre‑B acute lymphocytic leukemia cell line, Jurkat human acute T cell leukemia cell line and mouse spleen tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse NPM1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5205) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for NPM1 at approximately 37 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.|
NPM1 (Nucleophosmin 1; also nucleolar protein 23) is a 40 kDa member of the nucleoplasmin family of proteins. It is found in the nucleus where it performs multiple functions. NPM1 normally associates with unduplicated centrosomes. After phosphorylation at Thr199, it dissociates from centrosomes, allowing for centrosome duplication. NPM1 also negatively regulates GCN5-dependent histone acetylation during mitosis. Human NPM1 is 294 amino acids (aa) in length. It contains a Met-rich region (aa 1‑9), two acidic regions (aa 120‑132 and 161‑188) and two NLSs (aa 152‑157 and 191‑197). Phosphorylation occurs at Ser4/10/70/254. Splice variants show a deletion of aa 195‑223, a two aa substitution for aa 258‑294 and an 11 aa substitution for aa 288‑294. Over aa 9‑158, human NPM1 is 97% aa identical to mouse NPM1.
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