|Detection of Human and Mouse PLC‑δ3 by Western Blot. Western blot shows lysates of HT‑29 human colon adenocarcinoma cell line, 293T human embryonic kidney cell line, Jurkat human acute T cell leukemia cell line, and NIH‑3T3 mouse embryonic fibroblast cell line. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse PLC‑δ3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5315) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). Specific bands were detected for PLC‑δ3 at approximately 100‑110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.|
PLC-δ3 (phospholipase C-delta 3) is an 105 kDa member of the δ-type PLC isozyme family. It is expressed in corneal epithelium, platelets, cardiac and skeletal muscle. PLC-δ3 preferentially hydrolyzes membrane PIP2. This generates IP3, which induces intracellular Ca++ release, and DAG, activating PKC. Human PLC-δ3 is 789 amino acids (aa) in length. It contains a plextrin homology domain that tethers the enzyme to the membrane (aa 63‑172), three EF-hand motifs that spatially orient the molecule (aa 182‑285), a catalytic region (aa 337‑644), and a C2 domain that secures the catalytic region to its substrate (aa 647‑752). There are multiple potential isoforms. There is an alternate start site at Met54, a 40 aa substitution for the C-terminal 248 aa, and a Leu substitution for aa 102‑109. Over aa 148‑344, human PLC-δ3 is 93% aa identical to mouse PLC-δ3.
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