Detection of Human and Mouse PLC‑δ3 by Western Blot.
Western blot shows lysates of HT‑29 human colon adenocarcinoma cell line, 293T human embryonic kidney cell line, Jurkat human acute T cell leukemia cell line, and NIH‑3T3 mouse embryonic fibroblast cell line. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse PLC‑δ3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5315) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). Specific bands were detected for PLC‑δ3 at approximately 100‑110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PLC-delta 3
PLC-δ3 (phospholipase C-delta 3) is an 105 kDa member of the δ-type PLC isozyme family. It is expressed in corneal epithelium, platelets, cardiac and skeletal muscle. PLC-δ3 preferentially hydrolyzes membrane PIP2. This generates IP3, which induces intracellular Ca++ release, and DAG, activating PKC. Human PLC-δ3 is 789 amino acids (aa) in length. It contains a plextrin homology domain that tethers the enzyme to the membrane (aa 63‑172), three EF-hand motifs that spatially orient the molecule (aa 182‑285), a catalytic region (aa 337‑644), and a C2 domain that secures the catalytic region to its substrate (aa 647‑752). There are multiple potential isoforms. There is an alternate start site at Met54, a 40 aa substitution for the C-terminal 248 aa, and a Leu substitution for aa 102‑109. Over aa 148‑344, human PLC-δ3 is 93% aa identical to mouse PLC-δ3.
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