Human/Mouse Plexin A1 Antibody

Catalog # Availability Size / Price Qty
AF4309
AF4309-SP
Detection of Mouse Plexin A1 by Western Blot.
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Product Details
Citations (5)
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Human/Mouse Plexin A1 Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects mouse Plexin A1 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant mouse Plexin A2 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Plexin A1
Ser28-Pro1242
Accession # P70206
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
See below
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Mouse Plexin A1 by Western Blot. View Larger

Detection of Mouse Plexin A1 by Western Blot. Western blot shows lysates of mouse embryonic heart tissue. PVDF Membrane was probed with 1 µg/mL of Mouse Plexin A1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4309) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Plexin A1 at approximately 200 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Immunocytochemistry Plexin A1 in Human Dendritic Cells. View Larger

Plexin A1 in Human Dendritic Cells. Plexin A1 was detected in immersion fixed immature human dendritic cells using Goat Anti-Mouse Plexin A1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4309) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Immunohistochemistry Plexin A1 in Mouse Spinal Cord. View Larger

Plexin A1 in Mouse Spinal Cord. Plexin A1 was detected in immersion fixed frozen sections of embryonic mouse spinal cord using Mouse Plexin A1 Affinity-purified Polyclonal Antibody (Catalog # AF4309) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Plexin A1

Plexin A1 (formerly Plexin 1) is a 200 kDa type I transmembrane protein that is a member of the Plexin family of Semaphorin signal transducers (1). Plexin signaling induces cytoskeletal remodeling, which mediates cell migration and axon repulsion (2). The mouse Plexin A1 cDNA encodes 1894 amino acids (aa) including a 27 aa signal sequence, a 1215 aa extracellular domain (ECD) with one Sema domain, a spacer, and four tandem IPT/TIG domains, a 21 aa transmembrane segment, and a 631 aa cytoplasmic domain (1). Within the ECD, human Plexin A1 shares 95%, 95%, 92%, 80% and 79% aa sequence identity with mouse, rat, bovine, chicken and Xenopus Plexin A1, respectively. The four mouse Plexin A molecules share 59‑67% aa identity with each other. Plexin A1 binds Class 3 (secreted) Semaphorins indirectly via Neuropilin (Npn)-1 and Npn-2, and binds transmembrane Semaphorin 6D directly (3‑5). Sema3A engagement of Plexin A1 and Npn-1 guides proprioceptive and sensory neurons during development, while Sema3B engagement guides floorplate neurons (5‑8). In contrast, T cell Sema6D engagement of dendritic cell Plexin A1 controls actin polymeration, which supports formation of immunological synapses and enhances the function of the dendritic cells (3, 4, 9). Complex formation with DAP12 allows Plexin A1 signaling through TREM family proteins (10, 11). However, the most striking effect of Plexin A1 deletion is on bone homeostasis, where Plexin A1-deficient mice show increased trabecular bone mass due to downregulated osteoclast differentiation (10). Plexin A1 and Sema6D are frequently expressed in malignant pleural mesothelioma, where they promote anchorage-independent growth through complexing with and activating VEGF R2 (12).

References
  1. Kameyama, T. et al. (1996) Biochem. Biophys. Res. Commun. 226:524.
  2. Kruger, R.P. et al. (2005) Nat. Rev. Mol. Cell Biol. 6:789.
  3. Takamatsu, H. et al. (2010) Cell. Mol. Immunol. 7:83.
  4. O’Connor, B.P. and J.P.Y. Ting (2008) Immunol. Res. 41:217.
  5. Takahashi, T. et al. (1999) Cell 99:59.
  6. Yoshida, Y. et al. (2006) Neuron 52:775.
  7. Toyofuku, T. et al. (2005) Nat. Neurosci. 8:1712.
  8. Nawabi, H. et al. (2010) Genes Dev. 24:396.
  9. Eun, S-Y. et al. (2006) J. Immunol. 177:4271.
  10. Takegahara, N. et al. (2006) Nat. Cell Biol. 8:615.
  11. Watarai, H. et al. (2008) Proc. Natl. Acad. Sci. USA 105:2993.
  12. Catalano, A. et al. (2009) Cancer Res. 69:1485.
Entrez Gene IDs
5361 (Human); 18844 (Mouse)
Alternate Names
NOV; NOVP; Plexin A1; PLEXIN-A1; PLXN1; PLXNA1

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Citations for Human/Mouse Plexin A1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Vinaxanthone inhibits Semaphorin3A induced axonal growth cone collapse in embryonic neurons but fails to block its growth promoting effects on adult neurons
    Authors: E Ivakhnitsk, MR Chin, D Siegel, VH Guaiquil
    Scientific Reports, 2021;11(1):13019.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  2. PlexinA1 deficiency in BALB/cAJ mice leads to excessive self-grooming and reduced prepulse inhibition
    Authors: MS Jahan, T Ito, S Ichihashi, T Masuda, MER Bhuiyan, I Takahashi, H Takamatsu, A Kumanogoh, T Tsuzuki, T Negishi, K Yukawa
    IBRO Reports, 2020;9(0):276-289.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  3. Synapse type-specific proteomic dissection identifies IgSF8 as a hippocampal CA3 microcircuit organizer
    Authors: N Apóstolo, SN Smukowski, J Vanderlind, G Condomitti, V Rybakin, J Ten Bos, L Trobiani, S Portegies, KM Vennekens, NV Gounko, D Comoletti, KD Wierda, JN Savas, J de Wit
    Nat Commun, 2020;11(1):5171.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. PlexinA1 is crucial for the midline crossing of callosal axons during corpus callosum development in BALB/cAJ mice
    Authors: MM Hossain, T Tsuzuki, K Sakakibara, F Imaizumi, A Ikegaya, M Inagaki, I Takahashi, T Ito, H Takamatsu, A Kumanogoh, T Negishi, K Yukawa
    PLoS ONE, 2019;14(8):e0221440.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  5. Semaphorin-5B Controls Spiral Ganglion Neuron Branch Refinement During Development
    Authors: JS Jung, KD Zhang, Z Wang, M McMurray, A Tkaczuk, Y Ogawa, R Hertzano, TM Coate
    J. Neurosci., 2019;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC

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