Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Western Blot, Immunocytochemistry

Cited:

Immunohistochemistry, Western Blot, Neutralization, Immunocytochemistry

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Plexin A1 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Plexin A1
Ser28-Pro1242
Accession # P70206

Specificity

Detects mouse Plexin A1 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant mouse Plexin A2 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human/Mouse Plexin A1 Antibody

Detection of Mouse Plexin A1 antibody by Western Blot.

Detection of Mouse Plexin A1 by Western Blot.

Western blot shows lysates of mouse embryonic heart tissue. PVDF Membrane was probed with 1 µg/mL of Mouse Plexin A1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4309) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Plexin A1 at approximately 200 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Plexin A1 antibody in Human Dendritic Cells by Immunocytochemistry (ICC).

Plexin A1 in Human Dendritic Cells.

Plexin A1 was detected in immersion fixed immature human dendritic cells using Goat Anti-Mouse Plexin A1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4309) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Plexin A1 antibody in Mouse Spinal Cord by Immunohistochemistry (IHC-Fr).

Plexin A1 in Mouse Spinal Cord.

Plexin A1 was detected in immersion fixed frozen sections of embryonic mouse spinal cord using Mouse Plexin A1 Affinity-purified Polyclonal Antibody (Catalog # AF4309) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Detection of Mouse Plexin A1 by Western Blot

Detection of Mouse Plexin A1 by Western Blot

Localization of PlexinA1 and Npn1 in the coronal sections of WT and PlexinA1 KO brains at E16.5 and E17.5.(A) Immunohistochemistry with anti-PlexinA1 antibody revealed the localization of PlexinA1 only in WT brains at E16.5 and E17.5 (a, b) as well as the lack of PlexinA1 in PlexinA1 KO brains (c, d). Scale bars: 200 μm. (B, C) Western blot analysis using anti-PlexinA1 antibodies was performed with tissue lysates from the medial regions covering the cingulate cortex, axons from the cingulate and neocortex, and the CC of E16.5 and E17.5 brains. PlexinA1 protein was detected in WT brains (B; E16.5, C; E17.5) but not in PlexinA1 KO brains (B; E16.5, C; E17.5). (D) Immunohistochemistry with anti-Npn1 antibodies revealed localization of Npn1 in both WT (a; E16.5, b; E17.5) and PlexinA1 KO (c; E16.5, d; E17.5) brains at E16.5 and E17.5. Scale bars: 200 μm. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31430342), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse Plexin A1 by Western Blot

Detection of Mouse Plexin A1 by Western Blot

Localization of PlexinA1 and Npn1 in the coronal sections of WT and PlexinA1 KO brains at E16.5 and E17.5.(A) Immunohistochemistry with anti-PlexinA1 antibody revealed the localization of PlexinA1 only in WT brains at E16.5 and E17.5 (a, b) as well as the lack of PlexinA1 in PlexinA1 KO brains (c, d). Scale bars: 200 μm. (B, C) Western blot analysis using anti-PlexinA1 antibodies was performed with tissue lysates from the medial regions covering the cingulate cortex, axons from the cingulate and neocortex, and the CC of E16.5 and E17.5 brains. PlexinA1 protein was detected in WT brains (B; E16.5, C; E17.5) but not in PlexinA1 KO brains (B; E16.5, C; E17.5). (D) Immunohistochemistry with anti-Npn1 antibodies revealed localization of Npn1 in both WT (a; E16.5, b; E17.5) and PlexinA1 KO (c; E16.5, d; E17.5) brains at E16.5 and E17.5. Scale bars: 200 μm. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31430342), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse Plexin A1 by Immunocytochemistry/ Immunofluorescence

Detection of Mouse Plexin A1 by Immunocytochemistry/ Immunofluorescence

Expression of Sema3A, NGF and BNDF receptors in adult TG and embryonic DRG neurons. The expression of Sema3A receptors (NRP1, NRP2, PlexinA1 and PlexinA3) and the NGF receptors TrkA, the BDNF receptor TrkB, and the neuropeptide CGRP in isolated TG and DRG neurons was evaluated by immunofluorescence staining. Representative images of TG neurons treated with Sema3A (A) show expression of all receptors both in the cell body as well as in the growing neurites. We found that 100% of neurons express the receptors and the neuropeptide CGRP. Similarly, all of these receptors were also expressed in DRG neurons treated with NGF (B). However, 100% of the neurons expressed NRP1, NRP2, PlexinA3, TrkA and CGRP, while 75% of the DRG neurons expressed PlexinA3 and TrkB. In general, there were no major differences in Sema3A receptor expression between the adult and embryonic sensory neurons. Additionally, no differences were observed in the expression of the neuropeptide CGRP. See Table 1 in “Materials and methods” for antibody information. All images were taken at same magnification, scale bar = 25 μm. The background effect generated by the secondary antibody (staining without primary antibody) is shown on Supplementary Fig. S4). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/34155284), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse Plexin A1 by Immunocytochemistry/ Immunofluorescence

Detection of Mouse Plexin A1 by Immunocytochemistry/ Immunofluorescence

Expression of Sema3A, NGF and BNDF receptors in adult TG and embryonic DRG neurons. The expression of Sema3A receptors (NRP1, NRP2, PlexinA1 and PlexinA3) and the NGF receptors TrkA, the BDNF receptor TrkB, and the neuropeptide CGRP in isolated TG and DRG neurons was evaluated by immunofluorescence staining. Representative images of TG neurons treated with Sema3A (A) show expression of all receptors both in the cell body as well as in the growing neurites. We found that 100% of neurons express the receptors and the neuropeptide CGRP. Similarly, all of these receptors were also expressed in DRG neurons treated with NGF (B). However, 100% of the neurons expressed NRP1, NRP2, PlexinA3, TrkA and CGRP, while 75% of the DRG neurons expressed PlexinA3 and TrkB. In general, there were no major differences in Sema3A receptor expression between the adult and embryonic sensory neurons. Additionally, no differences were observed in the expression of the neuropeptide CGRP. See Table 1 in “Materials and methods” for antibody information. All images were taken at same magnification, scale bar = 25 μm. The background effect generated by the secondary antibody (staining without primary antibody) is shown on Supplementary Fig. S4). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/34155284), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human/Mouse Plexin A1 Antibody

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed immature human dendritic cells

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed frozen sections of embryonic mouse spinal cord  

Western Blot

1 µg/mL
Sample: Mouse embryonic heart tissue

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Plexin A1

Plexin A1 (formerly Plexin 1) is a 200 kDa type I transmembrane protein that is a member of the Plexin family of Semaphorin signal transducers (1). Plexin signaling induces cytoskeletal remodeling, which mediates cell migration and axon repulsion (2). The mouse Plexin A1 cDNA encodes 1894 amino acids (aa) including a 27 aa signal sequence, a 1215 aa extracellular domain (ECD) with one Sema domain, a spacer, and four tandem IPT/TIG domains, a 21 aa transmembrane segment, and a 631 aa cytoplasmic domain (1). Within the ECD, human Plexin A1 shares 95%, 95%, 92%, 80% and 79% aa sequence identity with mouse, rat, bovine, chicken and Xenopus Plexin A1, respectively. The four mouse Plexin A molecules share 59‑67% aa identity with each other. Plexin A1 binds Class 3 (secreted) Semaphorins indirectly via Neuropilin (Npn)-1 and Npn-2, and binds transmembrane Semaphorin 6D directly (3‑5). Sema3A engagement of Plexin A1 and Npn-1 guides proprioceptive and sensory neurons during development, while Sema3B engagement guides floorplate neurons (5‑8). In contrast, T cell Sema6D engagement of dendritic cell Plexin A1 controls actin polymeration, which supports formation of immunological synapses and enhances the function of the dendritic cells (3, 4, 9). Complex formation with DAP12 allows Plexin A1 signaling through TREM family proteins (10, 11). However, the most striking effect of Plexin A1 deletion is on bone homeostasis, where Plexin A1-deficient mice show increased trabecular bone mass due to downregulated osteoclast differentiation (10). Plexin A1 and Sema6D are frequently expressed in malignant pleural mesothelioma, where they promote anchorage-independent growth through complexing with and activating VEGF R2 (12).

References

  1. Kameyama, T. et al. (1996) Biochem. Biophys. Res. Commun. 226:524.
  2. Kruger, R.P. et al. (2005) Nat. Rev. Mol. Cell Biol. 6:789.
  3. Takamatsu, H. et al. (2010) Cell. Mol. Immunol. 7:83.
  4. O’Connor, B.P. and J.P.Y. Ting (2008) Immunol. Res. 41:217.
  5. Takahashi, T. et al. (1999) Cell 99:59.
  6. Yoshida, Y. et al. (2006) Neuron 52:775.
  7. Toyofuku, T. et al. (2005) Nat. Neurosci. 8:1712.
  8. Nawabi, H. et al. (2010) Genes Dev. 24:396.
  9. Eun, S-Y. et al. (2006) J. Immunol. 177:4271.
  10. Takegahara, N. et al. (2006) Nat. Cell Biol. 8:615.
  11. Watarai, H. et al. (2008) Proc. Natl. Acad. Sci. USA 105:2993.
  12. Catalano, A. et al. (2009) Cancer Res. 69:1485.

Alternate Names

NOVP, PLXN1, PLXNA1

Entrez Gene IDs

5361 (Human); 18844 (Mouse)

Gene Symbol

PLXNA1

UniProt

P70206

Additional Plexin A1 Products

Product Documents for Human/Mouse Plexin A1 Antibody

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Product Specific Notices for Human/Mouse Plexin A1 Antibody

For research use only

Citations for Human/Mouse Plexin A1 Antibody

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