Human/Mouse PRMT1 Antibody

Detection of Human and Mouse PRMT1 by Western Blot.

Western blot shows lysates of 293T human embryonic kidney cell line and Hepa 1-6 mouse hepatoma cell line. PVDF Membrane was probed with 0.5 µg/mL of Goat Anti-Human PRMT1 Polyclonal Antibody (Catalog # AF6016) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for PRMT1 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Detection of PRMT1 by Western Blot

Loss of UBAP2L reduces tumor growth by decreasing global translation.a Representative anti-puromycin immunoblot (top) and densitometric analysis (bottom) of extracts from puromycin-treated MIA PaCa-2 cells knocked down using siRNAs against UBAP2L, PRMT1, or a non-targeting control (n = 3 biological replicates). b Representative immunoblot from two independent experiments showing Ubap2l levels in FC1245 parental and Ubap2l KO cells. c Cell confluence determined using IncuCyte software from phase-contrast images of FC1245 parental or Ubap2l KO cells (n = 3 independent experiments). d mRNA expression of the indicated rRNAs, normalized to Gapdh, in FC1245 parental, Prmt1 KO, and Ubap2l KO cells (n = 3 biological replicates). e Representative immunoblot (top) and quantification (bottom) showing Rpl31 protein levels, normalized to Vinculin, in FC1245 parental and Ubap2l KO cells (n = 3). f Tumor weights from mice orthotopically transplanted with FC1245 parental or Ubap2l KO cells (n = 3). g Representative images (left) and quantification (right) of tumor sections of FC1245 parental and Ubap2l KO tumors stained with Rpl31 (n = 6 from 2 fields per sample; scale bar: 100 μm). Data represent means ± SEM in (a, d, e–g), and means ± SD in (c). One-way ANOVA followed by Tukey’s multiple comparison test was performed in (a), unpaired two-tailed t-test in (c) and (e–g), and two-way ANOVA with Dunnett’s multiple comparison test in (d). ns: not significant. Source data are provided as a Source Data file. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37673892), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of PRMT1 by Western Blot

hnRNP F regulates PRMT1 levels to control cellular proliferation. A Venn diagram showing the overlap among eCLIP targets in AA0779E cells, genes downregulated upon HNRNPF knockdown in AA0779E cells, and genes downregulated upon Hnrnpf KO in mouse FC1245 cells. b Browser tracks displaying hnRNP F binding to PRMT1 mRNA; 3’UTR is boxed in red. c RT-qPCR showing expression levels of HNRNPF and PRMT1, normalized to GAPDH, in MIA PaCa-2 cells knocked down with siRNA against HNRNPF or a non-targeting control (n = 3 biological replicates). d Representative immunoblot (left) and quantification (right) showing Prmt1 levels in FC1245 parental and Hnrnpf KO cells (n = 3 biological replicates). e RT-qPCR showing levels of an exogenously expressed luciferase gene containing the 3’UTR of Prmt1, normalized to RFP driven by an independent promoter on the same plasmid, in FC1245 parental, Hnrnpf KO, or Hnrnpf KO cells rescued by exogenous HNRNPF re-expression (n = 6 biological replicates). f Representative immunoblot from two independent experiments showing Prmt1 and hnRNP F levels in FC1245 parental, Hnrnpf KO, or Hnrnpf KO cells rescued with re-expression of exogenous Prmt1. g Cell confluence determined using IncuCyte software from phase-contrast images of FC1245 parental, Hnrnpf KO, or Hnrnpf KO cells rescued with re-expression of exogenous Prmt1 (n = 3 independent experiments). Data represent means ± SEM in (c–e) and (g). Unpaired two-tailed t-test with two-stage step-up multiple comparison correction was performed in (c), one-way ANOVA followed by Tukey’s multiple comparisons test in (d, e), and one-way ANOVA with Sidak’s multiple comparisons test in (g). ns: not significant. Source data are provided as a Source Data file. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37673892), licensed under a CC-BY license. Not internally tested by R&D Systems.