Key Product Details

Species Reactivity

Human, Mouse

Applications

Knockout Validated, Western Blot, Intracellular Staining by Flow Cytometry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Mouse IgG1 Clone # 217702R
View all PTEN Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human PTEN
Thr2-Val403
Accession # P60484

Specificity

Detects human PTEN in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human/Mouse PTEN Antibody

Detection of Mouse PTEN by Western Blot.

Western blot shows lysates of 3T3‑L1 mouse embryonic fibroblast adipose-like cell line and C2C12 mouse myoblast cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human/Mouse PTEN Monoclonal Antibody (Catalog # MAB847R) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for PTEN at approximately 54 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Detection of Mouse PTEN by Western Blot.

Western blot shows lysates of mouse brain. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human/Mouse PTEN Monoclonal Antibody (Catalog # MAB847R) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for PTEN at approximately 54 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Detection of PTEN in Human U937 cell line by Flow Cytometry.

Human U937 histiocytic lymphoma cell line was stained with Mouse Anti-Human/Mouse PTEN Monoclonal Antibody (Catalog # MAB847R, filled histogram) or isotype control antibody (MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2 Secondary Antibody (F0102B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (FC005). Staining was performed using our protocol for Staining Intracellular Molecules.

Western Blot Shows Human PTEN Specificity by Using Knockout Cell Line.

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and human PTEN knockout cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human/Mouse PTEN Monoclonal Antibody (Catalog # MAB847R) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for PTEN at approximately 54 kDa (as indicated) in the parental HeLa human cervical epithelial carcinoma cell line, but is not detectable in knockout HeLa human cervical epithelial carcinoma cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Applications for Human/Mouse PTEN Antibody

Application
Recommended Usage

Intracellular Staining by Flow Cytometry

0.25 µg/106 cells
Sample: Human U937 histiocytic lymphoma cell line

Knockout Validated

PTEN is specifically detected in HeLa human cervical epithelial carcinoma parental cell line but is not detectable in PTEN knockout HeLa human cervical epithelial carcinoma cell line.

Western Blot

2 µg/mL
Sample: 3T3‑L1 mouse embryonic fibroblast adipose-like cell line, C2C12 mouse myoblast cell line and mouse brain

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: PTEN

The tumor suppressor gene PTEN (phosphatase and tensin homolog deleted on chromosome 10), also known as MMAC1 (mutated in multiple advanced cancers 1), encodes a phosphatase that contains the catalytic signature motif (HCxxGxxRS/T) found in all members of the protein tyrosine phosphatase family. In vitro, the recombinant PTEN has both lipid phosphatase and protein phosphatase activities (1, 2). Interestingly, accumulating evidence has shown that the tumor suppressor activity of PTEN relies on its ability to dephosphorylate phosphatidylinositol (3,4,5)-triphosphate specifically at position 3 of the inositol ring (3). This activity reduces the levels of phosphatidylinositol (3,4,5)-triphosphate which is specifically produced from phosphatidylinositol (4,5)-diphosphate by PI 3-kinase upon activation by a variety of stimuli. Therefore, PTEN antagonizes PI 3-kinase-induced downstream signaling events and cellular processes including cell growth, apoptosis and cell motility. In vivo, the importance of PTEN catalytic activity in its tumor suppressor functions is underscored by the fact that the majority of PTEN missense mutations detected in tumor specimens target the phosphatase domain and cause a loss in PTEN phosphatase activity (4).

References

  1. Maehama, T. and J. Dixon (1998) J. Biol. Chem. 273:13375.
  2. Das, S. et al. (2003) Proc. Natl. Acad. Sci. USA 100:7491.
  3. Myers, M. et al. (1998) Proc. Natl. Acad. Sci. USA 95:13513.
  4. Waite, K. and C. Eng (2002) Am. J. Hum. Genet. 70:829.

Long Name

Phosphatase and Tensin Homolog Deleted on Chromosome 10

Alternate Names

MMAC1

Entrez Gene IDs

5728 (Human); 19211 (Mouse); 50557 (Rat)

Gene Symbol

PTEN

UniProt

P60484 (Human)

Additional PTEN Products

Product Documents for Human/Mouse PTEN Antibody

Certificate of Analysis

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Product Specific Notices for Human/Mouse PTEN Antibody

For research use only

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