Human/Mouse/Rat 11 beta-HSD1 Antibody Summary
Asn24-Lys292
Accession # P28845
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human/Mouse/Rat 11 beta -HSD1 by Western Blot. Western blot shows lysates of human, mouse and rat liver tissue. PVDF membrane was probed with 0.5 µg/mL of Human/Mouse/Rat 11 beta -HSD1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3397) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for 11 beta -HSD1 at approximately 36 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 2.
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Detection of 11 beta-HSD1 by Western Blot Effect of S100A16 on 11 beta -HSD1 expression Adipose, liver, and muscle tissues were removed from C57BL/6, S100A16Tg/Tg, and S100A16KO/+ mice, and proteins were extracted. (A) Analysis of S100A16 and 11 beta -HSD1 protein levels by Western blotting. (B) Relative expression of S100A16 and 11 beta -HSD1 based on grayscale analysis with alpha -tubulin as a control. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/31399502), licensed under a CC-BY license. Not internally tested by R&D Systems.
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Detection of 11 beta-HSD1 by Western Blot Lipid and triglyceride content increased in Hep1-6 cells that overexpressed 11 beta -HSD1. (A) Western blot showing the expression of 11 beta -HSD1 and (B) relative protein levels (n = 5). (C) Oil red O staining of Hep1-6 cells with 5% BSA or 300 μM OA/PA treatment. Box indicates magnified field of view. (D) Measurement of triglyceride content in Hep1-6 cells with the same treatment as (C) (n = 3). **P<0.01, ***P<0.001. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35637957), licensed under a CC-BY license. Not internally tested by R&D Systems.
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Detection of 11 beta-HSD1 by Western Blot Lipogenesis pathway is activated by overexpression of 11 beta -HSD1. (A) Western blot detection of proteins related to the lipid biosynthesis, oxidation, secretion, uptake, and hydrolysis pathways in 11 beta -HSD1 overexpressed or GFP overexpressed Hep1-6 cells exposed or not to 0.25 μM BVT2733 and (B) relative protein levels (n = 3). (C) Oil red O staining of Hep1-6 cells and treatments from (A). (D) Measurement of triglyceride levels in Hep1-6 cells and treatments from (A) (n = 3). (E) Western blot showing the expression levels of lipogenesis pathway regulatory proteins in Hep1-6 cells and treatments from (A), and (F) relative protein levels (n = 3). *P<0.05, **P<0.01, ***P<0.001. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35637957), licensed under a CC-BY license. Not internally tested by R&D Systems.
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Detection of 11 beta-HSD1 by Western Blot The phenotype of hepatocyte-specific 11 beta -HSD1 transgenic mice (TGM). (A) Western blot showing the expression of 11 beta -HSD1 in liver of Con vs TGM (n = 3). (B) Body weights (n = 10). (C) Liver weights (n = 10). (D) The statistical analysis of liver weight/body weight ratios (n = 10). (E) Oil red O staining of liver tissues. (F) Measurement of triglyceride levels in livers of Con vs TGM (n = 8). (G) Western blot detection of proteins related to lipogenesis and (H) relative protein levels (n = 3). (I) Western blot showing the expression levels of the GR and p-GR proteins in the nucleus and cytoplasm. *P<0.05, **P<0.01, ***P<0.001. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35637957), licensed under a CC-BY license. Not internally tested by R&D Systems.
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Detection of 11 beta-HSD1 by Western Blot Lipogenesis pathway is activated in primary hepatocytes derived from TGM. (A) Oil red O staining of primary hepatocytes of Con vs TGM. Scale bars: 50 μm. (B) Measurement of triglyceride content in primary hepatocytes from Con vs TGM (n = 3). (C) Western blot showing the expression levels of lipogenesis pathway regulatory proteins in primary hepatocytes of Con vs TGM, treated or not with 0.25 μM BVT2733 and (D) relative protein levels (n = 3). *P<0.05, **P<0.01, ***P<0.001. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35637957), licensed under a CC-BY license. Not internally tested by R&D Systems.
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Detection of 11 beta-HSD1 by Western Blot Lipid and triglyceride content increased in Hep1-6 cells that overexpressed 11 beta -HSD1. (A) Western blot showing the expression of 11 beta -HSD1 and (B) relative protein levels (n = 5). (C) Oil red O staining of Hep1-6 cells with 5% BSA or 300 μM OA/PA treatment. Box indicates magnified field of view. (D) Measurement of triglyceride content in Hep1-6 cells with the same treatment as (C) (n = 3). **P<0.01, ***P<0.001. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35637957), licensed under a CC-BY license. Not internally tested by R&D Systems.
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Detection of 11 beta-HSD1 by Western Blot The phenotype of hepatocyte-specific 11 beta -HSD1 transgenic mice (TGM). (A) Western blot showing the expression of 11 beta -HSD1 in liver of Con vs TGM (n = 3). (B) Body weights (n = 10). (C) Liver weights (n = 10). (D) The statistical analysis of liver weight/body weight ratios (n = 10). (E) Oil red O staining of liver tissues. (F) Measurement of triglyceride levels in livers of Con vs TGM (n = 8). (G) Western blot detection of proteins related to lipogenesis and (H) relative protein levels (n = 3). (I) Western blot showing the expression levels of the GR and p-GR proteins in the nucleus and cytoplasm. *P<0.05, **P<0.01, ***P<0.001. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35637957), licensed under a CC-BY license. Not internally tested by R&D Systems.
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Detection of 11 beta-HSD1 by Western Blot Lipogenesis pathway is activated in primary hepatocytes derived from TGM. (A) Oil red O staining of primary hepatocytes of Con vs TGM. Scale bars: 50 μm. (B) Measurement of triglyceride content in primary hepatocytes from Con vs TGM (n = 3). (C) Western blot showing the expression levels of lipogenesis pathway regulatory proteins in primary hepatocytes of Con vs TGM, treated or not with 0.25 μM BVT2733 and (D) relative protein levels (n = 3). *P<0.05, **P<0.01, ***P<0.001. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35637957), licensed under a CC-BY license. Not internally tested by R&D Systems.
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Detection of 11 beta-HSD1 by Western Blot Lipogenesis pathway is activated by overexpression of 11 beta -HSD1. (A) Western blot detection of proteins related to the lipid biosynthesis, oxidation, secretion, uptake, and hydrolysis pathways in 11 beta -HSD1 overexpressed or GFP overexpressed Hep1-6 cells exposed or not to 0.25 μM BVT2733 and (B) relative protein levels (n = 3). (C) Oil red O staining of Hep1-6 cells and treatments from (A). (D) Measurement of triglyceride levels in Hep1-6 cells and treatments from (A) (n = 3). (E) Western blot showing the expression levels of lipogenesis pathway regulatory proteins in Hep1-6 cells and treatments from (A), and (F) relative protein levels (n = 3). *P<0.05, **P<0.01, ***P<0.001. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35637957), licensed under a CC-BY license. Not internally tested by R&D Systems.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: 11 beta-HSD1
Hydoxysteroid (11-beta) Dehydrogenase, type I 11-beta-hydroxysteroid dehydrogenase 1(11 beta -HSD1, HSD11B1 or HSD1) is a microsomal glycoprotein enzyme, with an apparent mass of 36 kDa, that catalyzes the conversion of the stress hormone cortisol to the inactive metabolite cortisone. 11 beta -HSD1 can also catalyze the reverse reaction, the conversion of cortisone to cortisol. 11 beta -HSD1 has been detected in a wide range of rat and human tissues, including lung and testis, but highest amounts are expressed in the liver. Human 11 beta -HSD1 shares 79 and 77% sequence identity with mouse and rat 11 beta -HSD1, respectively. There are at least 2 isoforms of 11 beta -HSD. The other isoform, type II, is expressed predominantly in the kidney and placenta and catalyzes only the 11-beta-dehydrogenation reaction. High levels of cortisol can lead to visceral obesity and particular defects in the expression or function of 11 beta -HSD1 have also been associated with insulin resistance and cognitive function.
Product Datasheets
Citations for Human/Mouse/Rat 11 beta-HSD1 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 7
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S100A16-induced adipogenesis is associated with up-regulation of 11 beta -hydroxysteroid dehydrogenase type 1 (11 beta -HSD1)
Authors: Rihua Zhang, Jing Bao Kan, Shan Lu, Pei Tong, Jie Yang, Ling Xi et al.
Bioscience Reports
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The GR-gp78 Pathway is involved in Hepatic Lipid Accumulation Induced by Overexpression of 11 beta -HSD1
Authors: Mengliang Hu, Tingting Han, Qiyu Pan, Dongsheng Ni, Fengyi Gao, Liying Wang et al.
International Journal of Biological Sciences
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Corticosterone and Glucocorticoid Receptor in the Cortex of Rats during Aging-The Effects of Long-Term Food Restriction
Authors: V Tesic, J Ciric, I Jovanovic, N Zogovic, D Milanovic, S Kanazir, M Perovic
Nutrients, 2021-12-17;13(12):.
Species: Rat
Sample Types: Cell Lysates
Applications: Western Blot -
NAFLD Aggravates Septic Shock Due to Inadequate Adrenal Response and 11beta-HSDs Dysregulation in Rats
Authors: HC Huang, MH Tsai, FY Lee, TY Lin, CC Chang, CL Chuang, SJ Hsu, MC Hou, YH Huang
Pharmaceutics, 2020-04-28;12(5):.
Species: Rat
Sample Types: Cell Lysates
Applications: Western Blot -
S100A16-induced adipogenesis is associated with up-regulation of 11 beta -hydroxysteroid dehydrogenase type 1 (11 beta -HSD1)
Authors: Rihua Zhang, Jing Bao Kan, Shan Lu, Pei Tong, Jie Yang, Ling Xi et al.
Bioscience Reports
Species: Mouse
Sample Types: Cell Lysates, Tissue Homogenates
Applications: Western Blot -
Saturated fatty acids in human visceral adipose tissue are associated with increased 11- beta-hydroxysteroid-dehydrogenase type 1 expression.
Authors: Petrus P, Rosqvist F, Edholm D, Mejhert N, Arner P, Dahlman I, Ryden M, Sundbom M, Riserus U
Lipids Health Dis, 2015-05-02;14(0):42.
Species: Human
Sample Types: Tissue Homogenates
Applications: Western Blot -
Identification of cytochrome P450 2C2 protein complexes in mouse liver.
Authors: Li B, Yau P, Kemper B
Proteomics, 2011-08-01;11(16):3359-68.
Species: Rabbit
Sample Types: Recombinant Protein
Applications: Immunoprecipitation
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