Human/Mouse/Rat Amphiphysin/AMPH Antibody

Catalog # Availability Size / Price Qty
AF6527
AF6527-SP
Human/Mouse/Rat Amphiphysin/AMPH Antibody in Western Blot
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Human/Mouse/Rat Amphiphysin/AMPH Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects Amphiphysin/AMPH in Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human Amphiphysin/AMPH
Asn204-Gly300
Accession # P49418
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Human, Mouse, and Rat Amphiphysin/AMPH by Western Blot. View Larger

Detection of Human, Mouse, and Rat Amphiphysin/AMPH by Western Blot. Western blot shows lysates of human brain tissue, rat Schwann cells, and mouse brain tissue. PVDF Membrane was probed with 0.5 µg/mL of Human/Mouse/Rat Amphiphysin/AMPH Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6527) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Amphiphysin/AMPH at approximately 128 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Amphiphysin/AMPH

AMPH (Amphiphysin; also AMPH1) is a 128 kDa member of the Amphiphysin family, BAR superfamily of proteins. Although its calculated MW is only 78 kDa, it runs anomalously in SDS-Page at 125-128 kDa. It is expressed in neurons, Sertoli cells, possibly in anterior pituitary endocrine cells and apparently cardiac muscle. It appears to form heterodimers with AMPH2, and in this configuration, promotes clathrin-associated endocytic vesicle formation. Furthermore, and perhaps related, AMPH also interacts with N-WASP, initiating actin polymerization. Human AMPH is 695 amino acids (aa) in length. It contains an N-terminal BAR domain (aa 24-240) that binds lipids, a central region that binds AP2 (aa 322-340) and clathrin (aa 347-386), and a C-terminal SH3 domain (aa 622-695) that binds proline-rich ligands. There is one known 108 kDa splice variant that shows a deletion of aa 425-466. Over aa 204-300, human AMPH shares 98% aa identity with mouse AMPH.

Entrez Gene IDs
273 (Human); 218038 (Mouse); 60668 (Rat)
Alternate Names
AMPH; AMPH1; amphiphysin (Stiff-Man syndrome with breast cancer 128kDa autoantigen); amphiphysin (Stiff-Mann syndrome with breast cancer 128kD autoantigen); amphiphysin I; Amphiphysin

Product Datasheets

FAQs

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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