Detects human Annexin A1 in direct ELISAs and human, mouse, and rat Annexin A1 in Western blots. In direct ELISAs, approximately 25%‑50% cross-reactivity with recombinant human (rh) Annexin A2 is observed and no cross-reactivity with rhAnnexin A3, A6, or A11 is observed. In Western blots, no cross-reactivity with rhAnnexin A6 is observed.
Monoclonal Mouse IgG1 Clone # 686122
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant human Annexin A1 Met1-Asn346 Accession # P04083
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Detection of Human, Mouse, and Rat Annexin A1 by Western Blot.
Western blot shows lysates of Jurkat human acute T cell leukemia cell line, NIH‑3T3 mouse embryonic fibroblast cell line, and L6 rat myoblast cell line. PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-Human/Mouse Annexin A1 Monoclonal Antibody (Catalog # MAB37701) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Annexin A1 at approximately 39 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Detection of Human Annexin A1 by Simple WesternTM.
Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line, loaded at 0.2 mg/mL. A specific band was detected for Annexin A1 at approximately 45 kDa (as indicated) using 1 µg/mL of Mouse Anti-Human/Mouse/Rat Annexin A1 Monoclonal Antibody (Catalog # MAB37701). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Detection of Mouse Annexin A1 by Simple WesternTM.
Simple Western lane view shows lysates of NIH‑3T3 mouse embryonic fibroblast cell line, loaded at 0.2 mg/mL. A specific band was detected for Annexin A1 at approximately 45 kDa (as indicated) using 1 µg/mL of Mouse Anti-Human/Mouse/Rat Annexin A1 Monoclonal Antibody (Catalog # MAB37701). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Preparation and Storage
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Annexin A1
The Annexins are a family of Calcium-dependent phospholipid-binding proteins that are preferentially located on the cytosolic face of the plasma membrane. The Annexins have a molecular weight of approximately 35 to 40 kDa and consist of a unique amino terminal domain followed by a homologous C-terminal core domain containing the calcium-dependent phospholipid-binding sites. The C‑terminal domain is comprised of four 60‑70 amino acid repeats, known as annexin repeats or an endonexin fold (Annexin A6 contains 8 annexin repeats). The four annexin repeats form a highly alpha -helical, tightly packed disc known as the annexin domain, which binds to phospholipids in the membrane in a calcium-dependent manner. Members of the annexin family play a role in cytoskeletal interactions, phospholipase inhibition, regulation of cellular growth, and intracellular signal transduction pathways. Annexin A1 (ANXA1), also known as annexin I, lipocortin I, and calpactin II, is an ~ 40 kDa protein with phospholipase A2 inhibitory activity. Since phospholipase A2 is required for the biosynthesis of the potent mediators of inflammation, prostaglandins and leukotrienes, Annexin A1 may have anti-inflammatory activity. Human Annexin A1 shares 88 and 89% amino acid sequence identity with mouse and rat Annexin A1, respectively.
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