Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse, Rat, Porcine

Applications

Validated:

Western Blot, Simple Western

Cited:

Western Blot, Flow Cytometry, ELISA Capture, Microarray

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Annexin V Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Annexin V
Ala2-Asp320
Accession # P08758

Specificity

Detects human, mouse and rat Annexin V in Western blots. Detects human Annexin V in direct ELISAs.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human/Mouse/Rat Annexin V Antibody

Detection of Human, Mouse, and Rat Annexin V antibody by Western Blot.

Detection of Human, Mouse, and Rat Annexin V by Western Blot.

Western blot shows lysates of human lung tissue, mouse lung tissue, and rat lung tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Annexin V Antigen Affinity-purified Polyclonal Antibody (Catalog # AF399) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band was detected for Annexin V at approximately 32 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Human, Mouse, Rat Annexin V by Simple WesternTM.

Simple Western lane view shows lysates of Human lung, mouse lung, rat lung, loaded at 0.2 mg/mL. A specific band was detected for Annexin V at approximately 38 kDa (as indicated) using 12.5 µg/mL of Goat Anti-Human/Mouse/Rat Annexin V Antigen Affinity-purified Polyclonal Antibody (Catalog # AF399). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications for Human/Mouse/Rat Annexin V Antibody

Application
Recommended Usage

Simple Western

12.5 µg/mL
Sample: Human lung, mouse lung, rat lung

Western Blot

1 µg/mL
Sample: Human lung tissue, mouse lung tissue, and rat lung tissue

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Annexin V

Annexin V is a member of a calcium and phospholipid binding family of proteins with vascular anticoagulant activity. It is also known as PP4, PAP I, CPB-I, CaBP33, VACa, anchorin CII, lipocortin-V, endonexin II, and thromboplastin inhibitor.

Alternate Names

ANX5, ANXA5, ANXV

Entrez Gene IDs

308 (Human)

Gene Symbol

ANXA5

UniProt

P08758

Additional Annexin V Products

Product Documents for Human/Mouse/Rat Annexin V Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human/Mouse/Rat Annexin V Antibody

For research use only

Related Research Areas

Coagulation

Citations for Human/Mouse/Rat Annexin V Antibody

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