|Detection of Human, Mouse, and Rat Carbonic Anhydrase XIV/CA14 by Western Blot. Western blot shows lysates of human, mouse, and rat brain (cerebellum) tissue and Hepa 1‑6 mouse hepatoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat Carbonic Anhydrase XIV/CA14 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2504) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Carbonic Anhydrase XIV/CA14 at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.|
Detection of Mouse and Rat Carbonic Anhydrase XIV/CA14 by Simple WesternTM. Simple Western lane view shows lysates of mouse and rat brain (cerebellum) tissue and Hepa 1‑6 mouse hepatoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Carbonic Anhydrase XIV/CA14 at approximately 54 kDa (as indicated) using 10 µg/mL of Goat Anti-Human/Mouse/Rat Carbonic Anhydrase XIV/|
CA14 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2504) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the
12-230 kDa separation system.
Carbonic anhydrase (CA) catalyzes the reversible reaction of CO2 + H2O = HCO3- + H+, which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption (1). Topics in a CA meeting (6th International Conference on the CAs, June 20‑25, 2003, Slovakia) ranged from use of CAs as markers for tumor and hypoxia in clinic, as nutritional supplement in milk, and as a tool for CO2 removal and mosquito control in industry. Mouse CA14 is a type I membrane enzyme highly expressed in the kidney and heart, followed by the skeletal muscle, brain, lung and liver (2). The deduced amino acid sequence of mouse CA14 consists of a signal peptide (residues 1 to 15), an ectodomain (residues 16 to 290), a transmembrane domain (residues 291 to 311) and a cytoplamic region (residues 32 to 337). rmCA14 corresponds to the ectodomain and has activity as described in Activity Assay Protocol.
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